A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria

Detalhes bibliográficos
Autor(a) principal: Salcedo-Porras, Nicolas
Data de Publicação: 2022
Outros Autores: Oliveira, Pedro Lagerblad, Guarneri, Alessandra Aparecida, Lowenberger, Carl
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56776
Resumo: Centre for Cell Biology. Development and Disease. Department of Biological Sciences. Simon Fraser University. Burnaby, BC, Canada.
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spelling Salcedo-Porras, NicolasOliveira, Pedro LagerbladGuarneri, Alessandra AparecidaLowenberger, Carl2023-01-30T19:26:37Z2023-01-30T19:26:37Z2022SALCEDO-PORRAS, Nicolas et al. A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria. Parasit Vectors, v. 15, n. 1, 269, 2022. doi: 10.1186/s13071-022-05358-9.1756-3305https://www.arca.fiocruz.br/handle/icict/56776engBioMed CentralA fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactériainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCentre for Cell Biology. Development and Disease. Department of Biological Sciences. Simon Fraser University. Burnaby, BC, Canada.Instituto de Bioquímica Médica Leopoldo de Meis. Universidade Federal do Rio de Janeiro. Ilha do Fundão, RJ, Brazil.Vector Behavior and Pathogen Interaction Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Centre for Cell Biology, Development and Disease. Department of Biological Sciences. Simon Fraser University. Burnaby, BC, Canada.Background: Rhodnius prolixus is an important vector of Trypanosoma cruzi, the causal agent of Chagas disease in humans. Despite the medical importance of this and other triatomine vectors, the study of their immune responses has been limited to a few molecular pathways and processes. Insect immunity studies were first described for holometabolous insects such as Drosophila melanogaster, and it was assumed that their immune responses were conserved in all insects. However, study of the immune responses of triatomines and other hemimetabolous insects has revealed discrepancies between these and the Drosophila model. Methods: To expand our understanding of innate immune responses of triatomines to pathogens, we injected fifth instar nymphs of R. prolixus with the Gram-negative (Gr-) bacterium Enterobacter cloacae, the Gram-positive (Gr+) bacterium Staphylococcus aureus, or phosphate-buffered saline (PBS), and evaluated transcript expression in the fat body 8 and 24 h post-injection (hpi). We analyzed the differential expression of transcripts at each time point, and across time, for each treatment. Results: At 8 hpi, the Gr- bacteria-injected group had a large number of differentially expressed (DE) transcripts, and most of the changes in transcript expression were maintained at 24 hpi. In the Gr+ bacteria treatment, few DE transcripts were detected at 8 hpi, but a large number of transcripts were DE at 24 hpi. Unexpectedly, the PBS control also had a large number of DE transcripts at 24 hpi. Very few DE transcripts were common to the different treatments and time points, indicating a high specificity of the immune responses of R. prolixus to different pathogens. Antimicrobial peptides known to be induced by the immune deficiency pathway were induced upon Gr- bacterial infection. Many transcripts of genes from the Toll pathway that are thought to participate in responses to Gr+ bacteria and fungi were induced by both bacteria and PBS treatment. Pathogen recognition receptors and serine protease cascade transcripts were also overexpressed after Gr- bacteria and PBS injections. Gr- injection also upregulated transcripts involved in the metabolism of tyrosine, a major substrate involved in the melanotic encapsulation response to pathogens. Conclusions: These results reveal time-dependent pathogen-specific regulation of immune responses in triatomines, and hint at strong interactions between the immune deficiency and Toll pathways.Immune deficiency pathwayInnate immunitySerine protease inhibitorsSerine proteasesToll pathwayTranscriptomeTriatominesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56776/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALA fat body transcriptome analysis of the immune.pdfA fat body transcriptome analysis of the immune.pdfapplication/pdf1428308https://www.arca.fiocruz.br/bitstream/icict/56776/2/A%20fat%20body%20transcriptome%20analysis%20of%20the%20immune.pdfb123438aa5bdb3189d6ef3b8c27b904fMD52icict/567762023-01-30 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dc.title.en_US.fl_str_mv A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria
title A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria
spellingShingle A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria
Salcedo-Porras, Nicolas
Immune deficiency pathway
Innate immunity
Serine protease inhibitors
Serine proteases
Toll pathway
Transcriptome
Triatomines
title_short A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria
title_full A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria
title_fullStr A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria
title_full_unstemmed A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria
title_sort A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria
author Salcedo-Porras, Nicolas
author_facet Salcedo-Porras, Nicolas
Oliveira, Pedro Lagerblad
Guarneri, Alessandra Aparecida
Lowenberger, Carl
author_role author
author2 Oliveira, Pedro Lagerblad
Guarneri, Alessandra Aparecida
Lowenberger, Carl
author2_role author
author
author
dc.contributor.author.fl_str_mv Salcedo-Porras, Nicolas
Oliveira, Pedro Lagerblad
Guarneri, Alessandra Aparecida
Lowenberger, Carl
dc.subject.en.en_US.fl_str_mv Immune deficiency pathway
Innate immunity
Serine protease inhibitors
Serine proteases
Toll pathway
Transcriptome
Triatomines
topic Immune deficiency pathway
Innate immunity
Serine protease inhibitors
Serine proteases
Toll pathway
Transcriptome
Triatomines
description Centre for Cell Biology. Development and Disease. Department of Biological Sciences. Simon Fraser University. Burnaby, BC, Canada.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-01-30T19:26:37Z
dc.date.available.fl_str_mv 2023-01-30T19:26:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SALCEDO-PORRAS, Nicolas et al. A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria. Parasit Vectors, v. 15, n. 1, 269, 2022. doi: 10.1186/s13071-022-05358-9.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56776
dc.identifier.issn.en_US.fl_str_mv 1756-3305
identifier_str_mv SALCEDO-PORRAS, Nicolas et al. A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bactéria. Parasit Vectors, v. 15, n. 1, 269, 2022. doi: 10.1186/s13071-022-05358-9.
1756-3305
url https://www.arca.fiocruz.br/handle/icict/56776
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56776/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/56776/2/A%20fat%20body%20transcriptome%20analysis%20of%20the%20immune.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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