The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies

Detalhes bibliográficos
Autor(a) principal: Lisboa, Vinícius da Cunha
Data de Publicação: 2019
Outros Autores: Ribeiro-Alves, Marcelo, Corrêa, Raquel da Silva, Lopes, Isabelle Ramos, Mafort, Thiago Thomaz, Santos, Ana Paula Gomes Dos, Amadeu, Thaís Porto, Rufino, Rogério, Rodrigues, Luciana Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37127
Resumo: 2020-05-16
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spelling Lisboa, Vinícius da CunhaRibeiro-Alves, MarceloCorrêa, Raquel da SilvaLopes, Isabelle RamosMafort, Thiago ThomazSantos, Ana Paula Gomes DosAmadeu, Thaís PortoRufino, RogérioRodrigues, Luciana Silva2019-11-18T15:39:44Z2019-11-18T15:39:44Z2019LISBOA, Vinicius da Cunha et al. The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies. Journal of Clinical Microbiology, 20190095-1137https://www.arca.fiocruz.br/handle/icict/3712710.1128/JCM.00927-19engAmerican Society for MicrobiologyThe predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-05-16Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, BrazilLaboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, BrazilDepartment of Pulmonary and Tisiology Care, Pedro Ernesto University Hospital (HUPE)/UERJ, Rio de Janeiro, RJ, Brazil.Department of Pulmonary and Tisiology Care, Pedro Ernesto University Hospital (HUPE)/UERJ, Rio de Janeiro, RJ, Brazil.Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil.Department of Pulmonary and Tisiology Care, Pedro Ernesto University Hospital (HUPE)/UERJ, Rio de Janeiro, RJ, Brazil.Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil.Pleural tuberculosis (PlTB), a common form of extrapulmonary TB, remains as a challenge in the diagnosis among many causes of pleural effusion. We recently reported that the combinatorial analysis of interferon-gamma (IFN-γ), IFN-γ-inducible protein 10 (IP-10), and adenosine deaminase (ADA) from the pleural microenvironment was useful to distinguish pleural effusion caused by TB (microbiologically or not confirmed cases) among other etiologies. In this cross-sectional cohort study, a set of inflammatory mediators was quantified in blood and pleural fluid (PF) from exudative pleural effusion cases, including PlTB (n = 27) and non-PlTB (nTB; n = 25) patients. The levels of IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF, IP-10, TGF-β1, and ADA were determined using cytometric bead assay, ELISA or biochemical tests. IFN-γ, IP-10, TNF, TGF-β, and ADA quantified in PF showed significantly higher concentrations in PlTB patients when compared to nTB. When blood and PF were compared, we have identified significantly higher concentrations of IL-6 and IL-10 in PF, in both groups. TGF-β, solely, showed significantly increased levels in PF and blood from PlTB when both clinical specimens were compared to nTB patients. Principal components analysis (PCA) revealed a T helper type 1 (Th1) pattern mainly attributed to higher levels of IP-10, IFN-γ, TGF-β, and TNF in pleural cavity, which was distinct between PlTB and nTB. In conclusion, our findings showed a predominantly cellular immune response in PF from TB cases rather than other causes of exudative effusion, commonly considered in the differential diagnosis of PlTB.Pleural tuberculosisPleural effusionAdenosine deaminaseTh1 responseCytokines in pleural effusioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/37127/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALThe predominance of Th1_Marcelo_Ribeiro-Alves_INI_Lapclin-AIDS_2019.pdfThe predominance of Th1_Marcelo_Ribeiro-Alves_INI_Lapclin-AIDS_2019.pdfapplication/pdf1454424https://www.arca.fiocruz.br/bitstream/icict/37127/2/The%20predominance%20of%20Th1_Marcelo_Ribeiro-Alves_INI_Lapclin-AIDS_2019.pdf512b24fa1fcd9a0dc33a2af6b5958914MD52TEXTThe predominance of Th1_Marcelo_Ribeiro-Alves_INI_Lapclin-AIDS_2019.pdf.txtThe predominance of Th1_Marcelo_Ribeiro-Alves_INI_Lapclin-AIDS_2019.pdf.txtExtracted texttext/plain50363https://www.arca.fiocruz.br/bitstream/icict/37127/3/The%20predominance%20of%20Th1_Marcelo_Ribeiro-Alves_INI_Lapclin-AIDS_2019.pdf.txtcb2044a3ee46006a2f87b270eb7bf98cMD53icict/371272021-03-24 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dc.title.pt_BR.fl_str_mv The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies
title The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies
spellingShingle The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies
Lisboa, Vinícius da Cunha
Pleural tuberculosis
Pleural effusion
Adenosine deaminase
Th1 response
Cytokines in pleural effusion
title_short The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies
title_full The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies
title_fullStr The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies
title_full_unstemmed The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies
title_sort The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies
author Lisboa, Vinícius da Cunha
author_facet Lisboa, Vinícius da Cunha
Ribeiro-Alves, Marcelo
Corrêa, Raquel da Silva
Lopes, Isabelle Ramos
Mafort, Thiago Thomaz
Santos, Ana Paula Gomes Dos
Amadeu, Thaís Porto
Rufino, Rogério
Rodrigues, Luciana Silva
author_role author
author2 Ribeiro-Alves, Marcelo
Corrêa, Raquel da Silva
Lopes, Isabelle Ramos
Mafort, Thiago Thomaz
Santos, Ana Paula Gomes Dos
Amadeu, Thaís Porto
Rufino, Rogério
Rodrigues, Luciana Silva
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lisboa, Vinícius da Cunha
Ribeiro-Alves, Marcelo
Corrêa, Raquel da Silva
Lopes, Isabelle Ramos
Mafort, Thiago Thomaz
Santos, Ana Paula Gomes Dos
Amadeu, Thaís Porto
Rufino, Rogério
Rodrigues, Luciana Silva
dc.subject.en.pt_BR.fl_str_mv Pleural tuberculosis
Pleural effusion
Adenosine deaminase
Th1 response
Cytokines in pleural effusion
topic Pleural tuberculosis
Pleural effusion
Adenosine deaminase
Th1 response
Cytokines in pleural effusion
description 2020-05-16
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-11-18T15:39:44Z
dc.date.available.fl_str_mv 2019-11-18T15:39:44Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LISBOA, Vinicius da Cunha et al. The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies. Journal of Clinical Microbiology, 2019
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37127
dc.identifier.issn.pt_BR.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.00927-19
identifier_str_mv LISBOA, Vinicius da Cunha et al. The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies. Journal of Clinical Microbiology, 2019
0095-1137
10.1128/JCM.00927-19
url https://www.arca.fiocruz.br/handle/icict/37127
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/37127/1/license.txt
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