Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil

Detalhes bibliográficos
Autor(a) principal: Fernandes, Cloé Duarte
Data de Publicação: 1997
Outros Autores: Murta, Silvane Maria Fonseca, Cerávolo, Isabela Penna, Krug, Luciano Percival, Vidigal, Pedro G, Steindel, Mario, Nardi, Nanci, Romanha, Alvaro José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/60510
Resumo: SSMA/RS. Instituto de Pesquisas Biológicas. Porto Alegre, RS, Brasil
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spelling Fernandes, Cloé DuarteMurta, Silvane Maria FonsecaCerávolo, Isabela PennaKrug, Luciano PercivalVidigal, Pedro GSteindel, MarioNardi, NanciRomanha, Alvaro José2023-09-20T17:24:00Z2023-09-20T17:24:00Z1997FERNANDES, Cloé Duarte et al. Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil. Mem. Inst. Oswaldo Cruz, v. 92, n. 3, p. 343-351, 1997. doi.org/10.1590/S0074-027619970003000080074-0276https://www.arca.fiocruz.br/handle/icict/60510engMinistério da SaúdeCharacterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSSMA/RS. Instituto de Pesquisas Biológicas. Porto Alegre, RS, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrasilUniversidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, BrasilUniversidade Federal do Rio Grande do Sul. Departamento de Genética. Porto Alegre, RS, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrasilThirty-five Trypanosoma cruzi strains were isolated from chronic chagasic patients, triatomines and opossums from different municipalities of the State of Rio Grande do Sul. Parasites were characterized by means of mice infectivity, enzyme electrophoresis and randomly amplified polymorphic DNA (RAPD) analysis. Twenty-nine strains were isolated from chagasic patients, 4 from triatomines (2 from Triatoma infestans and 2 from Panstrongylus megistus) and 2 from opossums Didelphis albiventris. Thirty-three T. cruzi strains were of low and 2 strains of high virulence in mice. Both virulent strains were isolated from P. megistus. Isoenzyme analysis of the strains showed 3 different zymodemes. Eleven strains isolated from chagasic patients and 2 from D. albiventris were Z2. Eighteen strains from patients and 2 from T. infestans were ZB and 2 T. cruzi strains isolated from P. megistus were Z1. RAPD profiles obtained with 4 random primers showed a high genetic heterogeneity of the T. cruzi strains. Zymodeme 2 and ZB strains were the more polymorphic. A band sharing analysis of the RAPD profiles of Z2 and ZB strains using 3 primers, showed a very low percentage of shared bands, 20% among 13 ZB strains and 14% among 13 Z2 strains. According to the isoenzyme results, 3 T. cruzi populations were present in State of Rio Grande do Sul. Zymodeme 2 and ZB strains were found infecting man (domiciliar transmission cycle) whereas Z1 strains were found infecting the sylvatic vector P. megistus.Chagas’ diseaseTrypanosoma cruzirandomly amplified polymorphic DNAisoenzyme characterizationepidemiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60510/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFernandes et al. 1997 Memorias Inst Oswaldo Cruz.pdfFernandes et al. 1997 Memorias Inst Oswaldo Cruz.pdfapplication/pdf405995https://www.arca.fiocruz.br/bitstream/icict/60510/2/Fernandes%20et%20al.%201997%20Memorias%20Inst%20Oswaldo%20Cruz.pdfae71f4151dc8b99659eee8812ae4e83cMD52icict/605102023-09-20 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dc.title.en_US.fl_str_mv Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil
title Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil
spellingShingle Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil
Fernandes, Cloé Duarte
Chagas’ disease
Trypanosoma cruzi
randomly amplified polymorphic DNA
isoenzyme characterization
epidemiology
title_short Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil
title_full Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil
title_fullStr Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil
title_full_unstemmed Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil
title_sort Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil
author Fernandes, Cloé Duarte
author_facet Fernandes, Cloé Duarte
Murta, Silvane Maria Fonseca
Cerávolo, Isabela Penna
Krug, Luciano Percival
Vidigal, Pedro G
Steindel, Mario
Nardi, Nanci
Romanha, Alvaro José
author_role author
author2 Murta, Silvane Maria Fonseca
Cerávolo, Isabela Penna
Krug, Luciano Percival
Vidigal, Pedro G
Steindel, Mario
Nardi, Nanci
Romanha, Alvaro José
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Cloé Duarte
Murta, Silvane Maria Fonseca
Cerávolo, Isabela Penna
Krug, Luciano Percival
Vidigal, Pedro G
Steindel, Mario
Nardi, Nanci
Romanha, Alvaro José
dc.subject.en.en_US.fl_str_mv Chagas’ disease
Trypanosoma cruzi
randomly amplified polymorphic DNA
isoenzyme characterization
epidemiology
topic Chagas’ disease
Trypanosoma cruzi
randomly amplified polymorphic DNA
isoenzyme characterization
epidemiology
description SSMA/RS. Instituto de Pesquisas Biológicas. Porto Alegre, RS, Brasil
publishDate 1997
dc.date.issued.fl_str_mv 1997
dc.date.accessioned.fl_str_mv 2023-09-20T17:24:00Z
dc.date.available.fl_str_mv 2023-09-20T17:24:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FERNANDES, Cloé Duarte et al. Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil. Mem. Inst. Oswaldo Cruz, v. 92, n. 3, p. 343-351, 1997. doi.org/10.1590/S0074-02761997000300008
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/60510
dc.identifier.issn.en_US.fl_str_mv 0074-0276
identifier_str_mv FERNANDES, Cloé Duarte et al. Characterization of Trypanosoma cruzi Strains Isolated from Chronic Chagasic Patients, Triatomines and Opossums Naturally Infected from the State of Rio Grande do Sul, Brazil. Mem. Inst. Oswaldo Cruz, v. 92, n. 3, p. 343-351, 1997. doi.org/10.1590/S0074-02761997000300008
0074-0276
url https://www.arca.fiocruz.br/handle/icict/60510
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Ministério da Saúde
publisher.none.fl_str_mv Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/60510/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/60510/2/Fernandes%20et%20al.%201997%20Memorias%20Inst%20Oswaldo%20Cruz.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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