Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/56439 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil. |
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Roca, Tárcio PeixotoVillar, Livia MeloLima, Felipe Souza NogueiraVasconcelos, Mariana Pinheiro AlvesBorzacov, Lourdes Maria PinheiroSilva, Eugênia de Castro eLago, Bárbara Vieira doSilva, Mayara Torquato Lima daSouza, Luan Felipo BotelhoSalcedo, Juan Miguel VillalobosSantos, Alcione de Oliveira dosVieira, Deusilene Souza2023-01-11T17:27:23Z2023-01-11T17:27:23Z2022ROCA, Tárcio Peixoto et al. Article Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon. Viruses, v. 14, 2100, p. 1 - 15, Sept. 2022.1999-4915https://www.arca.fiocruz.br/handle/icict/5643910.3390/v14102100engMDPIMutaçãoGenótipoCaracterização clínicaMutationGenotypeClinical characterizationGenomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil.CEPEM/RO. Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.CEPEM/RO. Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.CEPEM/RO. Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Laboratório de Biotecnologia e Bioengenharia Estrutural. Rio de Janeiro, RJ, Brasil.Centro Universitário Aparício Carvalho. Escola de Ciências Biomédicas. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / CEPEM/RO. Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil.Centro Universitário Aparício Carvalho. Escola de Ciências Biomédicas. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil / CEPEM/RO. Centro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil.The emergence of clinically relevant mutations in the hepatitis B virus (HBV) genome has been a matter of great debate because of the possibility of escape from the host’s immune system, the potential to cause more severe progression of liver diseases and the emergence of treatment-resistant variants. Here we characterized the circulating variants of HBV in Rondônia State, in the north of Brazil. Serum samples of 62 chronic HBV carriers were subjected to PCR assays and clinical data were collected. Mutations and genotypes were characterized through direct sequencing. The findings show the presence of subgenotypes A1 (54.83%, 34/62), D3 (16.13%, 10/62), F2 (16.13%, 10/62), A2 (4.84%, 3/62), D2 (3.23%, 2/62), D1 (1.61%, 1/62), D4 (1.61%, 1/62) and F4 (1.61%, 1/62). Deletions in the pre-S2 region were found in 13.79% (8/58) of the samples, mutations in the S gene in 59.68% (37/62) and RT mutations in 48.39% (30/62). We found a variable genotypic distribution in different locations and important mutations related to immune escape and drug resistance in Western Amazonia, which contributed to genetic surveillance and provided important information to help control the disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALTarcioPRoca_BarbaraLago_etal_IOC_2022.pdfTarcioPRoca_BarbaraLago_etal_IOC_2022.pdfapplication/pdf2665660https://www.arca.fiocruz.br/bitstream/icict/56439/2/TarcioPRoca_BarbaraLago_etal_IOC_2022.pdfdf01748bb179d56a261643103973131cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56439/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/564392023-09-04 11:10:56.394oai:www.arca.fiocruz.br:icict/56439Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório 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| dc.title.en_US.fl_str_mv |
Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon |
| title |
Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon |
| spellingShingle |
Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon Roca, Tárcio Peixoto Mutação Genótipo Caracterização clínica Mutation Genotype Clinical characterization |
| title_short |
Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon |
| title_full |
Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon |
| title_fullStr |
Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon |
| title_full_unstemmed |
Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon |
| title_sort |
Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon |
| author |
Roca, Tárcio Peixoto |
| author_facet |
Roca, Tárcio Peixoto Villar, Livia Melo Lima, Felipe Souza Nogueira Vasconcelos, Mariana Pinheiro Alves Borzacov, Lourdes Maria Pinheiro Silva, Eugênia de Castro e Lago, Bárbara Vieira do Silva, Mayara Torquato Lima da Souza, Luan Felipo Botelho Salcedo, Juan Miguel Villalobos Santos, Alcione de Oliveira dos Vieira, Deusilene Souza |
| author_role |
author |
| author2 |
Villar, Livia Melo Lima, Felipe Souza Nogueira Vasconcelos, Mariana Pinheiro Alves Borzacov, Lourdes Maria Pinheiro Silva, Eugênia de Castro e Lago, Bárbara Vieira do Silva, Mayara Torquato Lima da Souza, Luan Felipo Botelho Salcedo, Juan Miguel Villalobos Santos, Alcione de Oliveira dos Vieira, Deusilene Souza |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Roca, Tárcio Peixoto Villar, Livia Melo Lima, Felipe Souza Nogueira Vasconcelos, Mariana Pinheiro Alves Borzacov, Lourdes Maria Pinheiro Silva, Eugênia de Castro e Lago, Bárbara Vieira do Silva, Mayara Torquato Lima da Souza, Luan Felipo Botelho Salcedo, Juan Miguel Villalobos Santos, Alcione de Oliveira dos Vieira, Deusilene Souza |
| dc.subject.other.en_US.fl_str_mv |
Mutação Genótipo Caracterização clínica |
| topic |
Mutação Genótipo Caracterização clínica Mutation Genotype Clinical characterization |
| dc.subject.en.en_US.fl_str_mv |
Mutation Genotype Clinical characterization |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Rondônia. Laboratório de Virologia Molecular. Porto Velho, RO, Brasil. |
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2022 |
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2022 |
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2023-01-11T17:27:23Z |
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2023-01-11T17:27:23Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
ROCA, Tárcio Peixoto et al. Article Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon. Viruses, v. 14, 2100, p. 1 - 15, Sept. 2022. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/56439 |
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1999-4915 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/v14102100 |
| identifier_str_mv |
ROCA, Tárcio Peixoto et al. Article Genomic Variability of Hepatitis B Virus Circulating in BrazilianWestern Amazon. Viruses, v. 14, 2100, p. 1 - 15, Sept. 2022. 1999-4915 10.3390/v14102100 |
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https://www.arca.fiocruz.br/handle/icict/56439 |
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eng |
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eng |
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MDPI |
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MDPI |
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