Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23053 |
Resumo: | National Institutes of Health [R01 TW009504, R25 TW009338, U01 AI088752, R01 AI052473 and D43 TW00919; Coordination for the Improvement of Higher Education Personnel (CAPES) from the Brazilian Ministry of Education; National Council of Scientific and Technological Development (CNPq) |
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Riediger, Irina NastassjaStoddard, Robyn A.Ribeiro, Guilherme de SousaNakatani, Sueli M.Moreira, Suzana D. R.Skraba, IreneBiondo, Alexander WelkerReis, Mitermayer Galvão dosHoffmaster, Alex R.Vinetz, Joseph M.Ko, Albert IcksangWunder Junior, Elsio Augusto2017-11-06T17:35:22Z2017-11-06T17:35:22Z2017RIEDIGER, Irina Nastassja et al. Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay. PLoS Neglected Tropical Diseases,p. 1-14, Sept. 2017.1935-2727https://www.arca.fiocruz.br/handle/icict/2305310.1371/journal.pntd.0005940National Institutes of Health [R01 TW009504, R25 TW009338, U01 AI088752, R01 AI052473 and D43 TW00919; Coordination for the Improvement of Higher Education Personnel (CAPES) from the Brazilian Ministry of Education; National Council of Scientific and Technological Development (CNPq)Central Laboratory of the State of Parana. Molecular Biology Section. Curitiba, PR, BrazilNational Center for Emerging and Zoonotic Infectious Diseases. Centers for Disease Control and Prevention. Atlanta, Georgia, USAFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Institute of Collective Health. Salvador, BA, BrazilCentral Laboratory of the State of Parana. Molecular Biology Section. Curitiba, PR, BrazilFederal University of the State of Paraná. Hospital das Clínicas. Curitiba, PR, BrazilCentral Laboratory of the State of Parana. Molecular Biology Section. Curitiba, PR, BrazilFederal University of the State of Paraná. Department of Veterinary Medicine. Curitiba, PR, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilNational Center for Emerging and Zoonotic Infectious Diseases. Centers for Disease Control and Prevention. Atlanta, Georgia, USAUniversity of California San Diego School of Medicine. Department of Medicine. Division of Infectious Diseases. La Jolla, California, USAFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, USAFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, USAWith a conservatively estimated 1 million cases of leptospirosis worldwide and a 5-10% fatality rate, the rapid diagnosis of leptospirosis leading to effective clinical and public health decision making is of high importance, and yet remains a challenge. Methodology Based on parallel, population-based studies in two leptospirosis-endemic regions in Brazil, a real-time PCR assay which detects lipL32, a gene specifically present in pathogenic Leptospira, was assessed for the diagnostic effectiveness and accuracy. Patients identified by active hospital-based surveillance in Salvador and Curitiba during large urban leptospirosis epidemics were tested. Real-time PCR reactions were performed with DNA-extracted samples obtained from 127 confirmed and 23 unconfirmed cases suspected of leptospirosis, 122 patients with an acute febrile illness other than leptospirosis, and 60 healthy blood donors. Principal findings The PCR assay had a limit of detection of 280 Leptospira genomic equivalents/mL. Sensitivity for confirmed cases was 61% for whole blood and 29% for serum samples. Sensitivity was higher (86%) for samples collected within the first 6 days after onset of illness compared to those collected after 7 days (34%). The real-time PCR assay was able to detect leptospiral DNA in blood from 56% of serological non-confirmed cases. The overall specificity of the assay was 99%. Conclusions These findings indicate that real-time PCR may be a reliable tool for early diagnosis of leptospirosis, which is decisive for clinical management of severe and life-threatening cases and for public health decision making.engPublic Library of ScienceLeptospiraLeptospiroseEpidemiaReação em cadeia da polimerase em tempo realLipoproteinasAdultoLeptospiraLeptospirosisEpidemicsReal-Time Polymerase Chain ReactionLipoproteinsAdultRapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23053/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRiediger IN Rapid, actionable....pdfRiediger IN Rapid, 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| dc.title.pt_BR.fl_str_mv |
Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay |
| title |
Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay |
| spellingShingle |
Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay Riediger, Irina Nastassja Leptospira Leptospirose Epidemia Reação em cadeia da polimerase em tempo real Lipoproteinas Adulto Leptospira Leptospirosis Epidemics Real-Time Polymerase Chain Reaction Lipoproteins Adult |
| title_short |
Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay |
| title_full |
Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay |
| title_fullStr |
Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay |
| title_full_unstemmed |
Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay |
| title_sort |
Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay |
| author |
Riediger, Irina Nastassja |
| author_facet |
Riediger, Irina Nastassja Stoddard, Robyn A. Ribeiro, Guilherme de Sousa Nakatani, Sueli M. Moreira, Suzana D. R. Skraba, Irene Biondo, Alexander Welker Reis, Mitermayer Galvão dos Hoffmaster, Alex R. Vinetz, Joseph M. Ko, Albert Icksang Wunder Junior, Elsio Augusto |
| author_role |
author |
| author2 |
Stoddard, Robyn A. Ribeiro, Guilherme de Sousa Nakatani, Sueli M. Moreira, Suzana D. R. Skraba, Irene Biondo, Alexander Welker Reis, Mitermayer Galvão dos Hoffmaster, Alex R. Vinetz, Joseph M. Ko, Albert Icksang Wunder Junior, Elsio Augusto |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Riediger, Irina Nastassja Stoddard, Robyn A. Ribeiro, Guilherme de Sousa Nakatani, Sueli M. Moreira, Suzana D. R. Skraba, Irene Biondo, Alexander Welker Reis, Mitermayer Galvão dos Hoffmaster, Alex R. Vinetz, Joseph M. Ko, Albert Icksang Wunder Junior, Elsio Augusto |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospira Leptospirose Epidemia Reação em cadeia da polimerase em tempo real Lipoproteinas Adulto |
| topic |
Leptospira Leptospirose Epidemia Reação em cadeia da polimerase em tempo real Lipoproteinas Adulto Leptospira Leptospirosis Epidemics Real-Time Polymerase Chain Reaction Lipoproteins Adult |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospira Leptospirosis Epidemics Real-Time Polymerase Chain Reaction Lipoproteins Adult |
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National Institutes of Health [R01 TW009504, R25 TW009338, U01 AI088752, R01 AI052473 and D43 TW00919; Coordination for the Improvement of Higher Education Personnel (CAPES) from the Brazilian Ministry of Education; National Council of Scientific and Technological Development (CNPq) |
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2017 |
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2017-11-06T17:35:22Z |
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2017-11-06T17:35:22Z |
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2017 |
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info:eu-repo/semantics/article |
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RIEDIGER, Irina Nastassja et al. Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay. PLoS Neglected Tropical Diseases,p. 1-14, Sept. 2017. |
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https://www.arca.fiocruz.br/handle/icict/23053 |
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1935-2727 |
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10.1371/journal.pntd.0005940 |
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RIEDIGER, Irina Nastassja et al. Rapid, actionable diagnosis of urban epidemic leptospirosis using a pathogenic Leptospira lipL32-based real-time PCR assay. PLoS Neglected Tropical Diseases,p. 1-14, Sept. 2017. 1935-2727 10.1371/journal.pntd.0005940 |
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Public Library of Science |
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