Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil

Detalhes bibliográficos
Autor(a) principal: Santos, Gina Peres Lima dos
Data de Publicação: 2012
Outros Autores: Burlandy, Fernanda Marcicano, Costa, Eliane Veiga da, Silva, Edson Elias da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/22755
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.
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spelling Santos, Gina Peres Lima dosBurlandy, Fernanda MarcicanoCosta, Eliane Veiga daSilva, Edson Elias da2017-10-03T15:10:27Z2017-10-03T15:10:27Z2012SANTOS, Gina Peres Lima dos; et al. Direct detection of enterovirus genome in cell-culture cerebrospinal fluid from aseptic meningitis cases in Brazil.. Virus Reviews & Research, v.17 , n.1-2, Jan./Dec, 2012.1519-2563https://www.arca.fiocruz.br/handle/icict/227552357-9323engSociedade Brasileira de VirologiaEnterovírusMeningite AssépticaReação em Cadeia da Polimerase em Tempo RealLíquido CefalorraquidianoEnterovirusRT-PCRAseptic MeningitisCSFDirect detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.Enteroviruses are the main etiologic agents involved in outbreaks and sporadic cases of aseptic meningitis. Viral isolation in permissive cells is a traditional method for diagnosis, but it is time consuming and may be unsuccessful due to low viral titers and because some enteroviruses do not replicate in routine cell cultures. Therefore, molecular techniques are increasingly being used for detection of these agents. In the present work, 267 cerebrospinal fluid (CSF) samples from aseptic meningitis and meningoencephalitis cases, received between 2008 and 2009, were tested by RT-PCR. These clinical samples were previously negative for virus isolation in cell culture. Enterovirus genome was detected in 59 CSF samples (22.1%) and identification was confirmed by partial nucleotide sequencing. Some demographics of the patients included in the study were analyzed. Direct detection of enterovirus genome from CSF is appropriate to increase sensitivity in aseptic meningitis and meningoencephalitis cases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/22755/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgina_santos_etal_IOC_2012.pdfgina_santos_etal_IOC_2012.pdfapplication/pdf105132https://www.arca.fiocruz.br/bitstream/icict/22755/2/gina_santos_etal_IOC_2012.pdffa4aac4e39d05de6e2fb2bfdd9860a4eMD52TEXTgina_santos_etal_IOC_2012.pdf.txtgina_santos_etal_IOC_2012.pdf.txtExtracted texttext/plain23104https://www.arca.fiocruz.br/bitstream/icict/22755/3/gina_santos_etal_IOC_2012.pdf.txtaaf819f1a043cff4984065e73b4d313eMD53icict/227552018-08-15 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dc.title.pt_BR.fl_str_mv Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil
title Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil
spellingShingle Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil
Santos, Gina Peres Lima dos
Enterovírus
Meningite Asséptica
Reação em Cadeia da Polimerase em Tempo Real
Líquido Cefalorraquidiano
Enterovirus
RT-PCR
Aseptic Meningitis
CSF
title_short Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil
title_full Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil
title_fullStr Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil
title_full_unstemmed Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil
title_sort Direct detection of enterovirus genome in cell-culture negative cerebrospinal fluid from aseptic meningitis in Brazil
author Santos, Gina Peres Lima dos
author_facet Santos, Gina Peres Lima dos
Burlandy, Fernanda Marcicano
Costa, Eliane Veiga da
Silva, Edson Elias da
author_role author
author2 Burlandy, Fernanda Marcicano
Costa, Eliane Veiga da
Silva, Edson Elias da
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos, Gina Peres Lima dos
Burlandy, Fernanda Marcicano
Costa, Eliane Veiga da
Silva, Edson Elias da
dc.subject.other.pt_BR.fl_str_mv Enterovírus
Meningite Asséptica
Reação em Cadeia da Polimerase em Tempo Real
Líquido Cefalorraquidiano
topic Enterovírus
Meningite Asséptica
Reação em Cadeia da Polimerase em Tempo Real
Líquido Cefalorraquidiano
Enterovirus
RT-PCR
Aseptic Meningitis
CSF
dc.subject.en.pt_BR.fl_str_mv Enterovirus
RT-PCR
Aseptic Meningitis
CSF
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-10-03T15:10:27Z
dc.date.available.fl_str_mv 2017-10-03T15:10:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Gina Peres Lima dos; et al. Direct detection of enterovirus genome in cell-culture cerebrospinal fluid from aseptic meningitis cases in Brazil.. Virus Reviews & Research, v.17 , n.1-2, Jan./Dec, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/22755
dc.identifier.issn.pt_BR.fl_str_mv 1519-2563
dc.identifier.eissn.none.fl_str_mv 2357-9323
identifier_str_mv SANTOS, Gina Peres Lima dos; et al. Direct detection of enterovirus genome in cell-culture cerebrospinal fluid from aseptic meningitis cases in Brazil.. Virus Reviews & Research, v.17 , n.1-2, Jan./Dec, 2012.
1519-2563
2357-9323
url https://www.arca.fiocruz.br/handle/icict/22755
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Virologia
publisher.none.fl_str_mv Sociedade Brasileira de Virologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/22755/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/22755/2/gina_santos_etal_IOC_2012.pdf
https://www.arca.fiocruz.br/bitstream/icict/22755/3/gina_santos_etal_IOC_2012.pdf.txt
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