Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/52061 |
Resumo: | Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - FUNCAP |
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Braga, Maria Flaviana Bezerra MoraisLeal, Antonio Linkoln Alves BorgesOliveira, Alison Honorio deCarneiro, Joara N alyda PereiraSantos, Antonia Thassya Lucas dosSales, Débora LimaFonseca, Victor Juno AlencarRocha, Janaina EsmeraldoFreitas, Thiago Sampaio deCampina, Fábia FerreiraCosta, Maria do SocorroPereira Junior, Francisco NascimentoAmaral, Wanderlei doRebelo, Ricardo AndradeSilva, Luiz Everson daRibeiro Filho, JaimeCoutinho, Henrique Douglas Melo2022-04-06T16:55:03Z2022-04-06T16:55:03Z2022BRAGA, Maria Flaviana Bezerra Morais et al. Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud. South African Journal of Botany, p. 1-9, 2022.0254-6299https://www.arca.fiocruz.br/handle/icict/5206110.1016/j.sajb.2022.03.017Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - FUNCAPUniversidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Universidade Federal do Cariri. Crato, CE, Brasil.Universidade Federal do Paraná. Curitiba, PR, Brasil.Universidade Regional de Blumenau. Blumenau, SC, Brasil.Universidade Federal do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Regional do Cariri. Crato, CE, Brasil.Antimicrobial resistance has emerged as a health public concern as the combat of resistant microorganisms has become increasingly challenging. Thus, considering the urgent need of developing new therapeutic alternatives, the present study aimed to investigate the chemical composition and evaluate the antimicrobial and drug enhancing potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud (EOLPx) against clinically relevant bacterial and fungal strains. Following collection, the leaves were dried at room temperature in a dark environment and the extraction was performed by hydrodistillation using a modified Clevenger-type apparatus. The oil was dried with anhydrous magnesium sulfate, filtered, concentrated in rotary evaporator, and 5 mL aliquots were stored in a refrigerator. The oil yield was expressed as a percentage of the dry weight of the plant material (w/w). The chemical characterization was performed by gas chromatography coupled to mass spectrometry (GC–MS) and flame ionization detector (FID). The antimicrobial analysis was carried out using the broth microdilution and half-maximal inhibitory concentrations (IC50), minimum inhibitory concentrations (MIC), and minimum fungicidal concentrations (MFC) were deterimined by subculture. To evaluate the drug enhancing potential, subinhibitory concentrations (MFC/16 and MIC/8) of the essential oil were combined with the antifungal drug fluconazole and the antibiotics norfloxacin, gentamicin and erythromycin. The impact of the in vitro treatment on fungal virulence was determined by observing morphological transition in wet chambers. The chemical analysis identified the presence of monoterpenes (58.8%) and sesquiterpenes (35.3%), including the monoterpene hydrocarbon myrcene (27.5%) as the major component. The in vitro treatment with the essential oil caused little impact on C. albicans growth, showing a weak fungicidal effect against the URM 4387 strain (at 8192 µg/mL), while the INCQS 40006 strain was not affected. In addition, the association of the EOLPx with fluconazole did not enhance the antifungal effect of this drug against none of the C. albicans strains. However, both the natural product and fluconazole inhibited fungal pleomorphism by both strains. While the essential oil MIC against all bacterial strains was higher than 813 μg/mL, it was found to enhance the activity of gentamicin against the SA and EC isolates as well as to potentiate the activity of erythromycin against the EC isolate. On the other hand, antagonistic effects were observed from its association with norfloxacin for both strains. In conclusion, the EOLPx showed antibiotic-enhancing effects in addition to inhibiting C. albicans morphological transition. Nevertheless, further research is required to better characterize the antimicrobial effects and mechanisms of action by the essential oil components.engElsevierPiperaceaeÓleos voláteisFolhas de plantaAnti-InfecciososPiperaceaeOils, volatilePlant leavesAnti-Infective AgentsPiperaceaeAceites volátilesHojas de la plantaAntiinfecciososPiperaceaeHuile essentielleFeuilles de planteAnti-infectieuxChemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steudinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52061/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBraga, Maria F. B. M. - Chemical characterization and antimicrobial.pdfBraga, Maria F. B. M. - Chemical characterization and antimicrobial.pdfapplication/pdf1666256https://www.arca.fiocruz.br/bitstream/icict/52061/2/Braga%2c%20Maria%20F.%20B.%20M.%20-%20Chemical%20characterization%20and%20antimicrobial.pdf74ca9a7a4ee0596484d4dbe3cc135c48MD52icict/520612023-03-15 14:34:29.188oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:29Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud |
| title |
Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud |
| spellingShingle |
Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud Braga, Maria Flaviana Bezerra Morais Piperaceae Óleos voláteis Folhas de planta Anti-Infecciosos Piperaceae Oils, volatile Plant leaves Anti-Infective Agents Piperaceae Aceites volátiles Hojas de la planta Antiinfecciosos Piperaceae Huile essentielle Feuilles de plante Anti-infectieux |
| title_short |
Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud |
| title_full |
Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud |
| title_fullStr |
Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud |
| title_full_unstemmed |
Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud |
| title_sort |
Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud |
| author |
Braga, Maria Flaviana Bezerra Morais |
| author_facet |
Braga, Maria Flaviana Bezerra Morais Leal, Antonio Linkoln Alves Borges Oliveira, Alison Honorio de Carneiro, Joara N alyda Pereira Santos, Antonia Thassya Lucas dos Sales, Débora Lima Fonseca, Victor Juno Alencar Rocha, Janaina Esmeraldo Freitas, Thiago Sampaio de Campina, Fábia Ferreira Costa, Maria do Socorro Pereira Junior, Francisco Nascimento Amaral, Wanderlei do Rebelo, Ricardo Andrade Silva, Luiz Everson da Ribeiro Filho, Jaime Coutinho, Henrique Douglas Melo |
| author_role |
author |
| author2 |
Leal, Antonio Linkoln Alves Borges Oliveira, Alison Honorio de Carneiro, Joara N alyda Pereira Santos, Antonia Thassya Lucas dos Sales, Débora Lima Fonseca, Victor Juno Alencar Rocha, Janaina Esmeraldo Freitas, Thiago Sampaio de Campina, Fábia Ferreira Costa, Maria do Socorro Pereira Junior, Francisco Nascimento Amaral, Wanderlei do Rebelo, Ricardo Andrade Silva, Luiz Everson da Ribeiro Filho, Jaime Coutinho, Henrique Douglas Melo |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Braga, Maria Flaviana Bezerra Morais Leal, Antonio Linkoln Alves Borges Oliveira, Alison Honorio de Carneiro, Joara N alyda Pereira Santos, Antonia Thassya Lucas dos Sales, Débora Lima Fonseca, Victor Juno Alencar Rocha, Janaina Esmeraldo Freitas, Thiago Sampaio de Campina, Fábia Ferreira Costa, Maria do Socorro Pereira Junior, Francisco Nascimento Amaral, Wanderlei do Rebelo, Ricardo Andrade Silva, Luiz Everson da Ribeiro Filho, Jaime Coutinho, Henrique Douglas Melo |
| dc.subject.other.pt_BR.fl_str_mv |
Piperaceae Óleos voláteis Folhas de planta Anti-Infecciosos |
| topic |
Piperaceae Óleos voláteis Folhas de planta Anti-Infecciosos Piperaceae Oils, volatile Plant leaves Anti-Infective Agents Piperaceae Aceites volátiles Hojas de la planta Antiinfecciosos Piperaceae Huile essentielle Feuilles de plante Anti-infectieux |
| dc.subject.en.pt_BR.fl_str_mv |
Piperaceae Oils, volatile Plant leaves Anti-Infective Agents |
| dc.subject.es.pt_BR.fl_str_mv |
Piperaceae Aceites volátiles Hojas de la planta Antiinfecciosos |
| dc.subject.fr.pt_BR.fl_str_mv |
Piperaceae Huile essentielle Feuilles de plante Anti-infectieux |
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Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico - FUNCAP |
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2022 |
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2022-04-06T16:55:03Z |
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2022-04-06T16:55:03Z |
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2022 |
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BRAGA, Maria Flaviana Bezerra Morais et al. Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud. South African Journal of Botany, p. 1-9, 2022. |
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https://www.arca.fiocruz.br/handle/icict/52061 |
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0254-6299 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.sajb.2022.03.017 |
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BRAGA, Maria Flaviana Bezerra Morais et al. Chemical characterization and antimicrobial potential of the essential oil obtained from the leaves of Piper xylosteoides (Kunth) Steud. South African Journal of Botany, p. 1-9, 2022. 0254-6299 10.1016/j.sajb.2022.03.017 |
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Elsevier |
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Elsevier |
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