Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28369 |
Resumo: | Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. |
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Barreira, André L.Takiya, Christina M.Castiglione, Raquel C.Maron-Gutierrez, TatianaBarbosa, Carolina M. L.Ornellas, Débora S.Verdoorn, Karine S.Pascarelli, Bernardo M.Borojevic, RadovanEinicker-Lamas, MarceloLeite Jr., MaurilioMorales, Marcelo M.Vieyra, Adalberto2018-08-27T12:24:16Z2018-08-27T12:24:16Z2009BARREIRA, André L. et al. Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction. Cell Physiol Biochem., v. 24, p.585-594, 2009.1015-8987https://www.arca.fiocruz.br/handle/icict/283691421-9778engKarger PublishersNefropatiasCélulas-Tronco AdultasObstrução ureteral unilateralReparação de tecidosCélulas mononucleares da medula ósseaBone marrow mononuclear cellsUnilateral ureteral obstructionTissue repairNephropathiesAdult stem cellsBone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstructioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Faculdade de Medicina. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.The growing number of patients suffering from chronic renal disease is a challenge for the development of innovative therapies. Benefits of cell therapy in acute renal diseases in animal models have been reported but seldom for chronic lesions. We present evidence for the improvement of renal morphology in a model of tubulointerstitial fibrosis. Wistar rats were submitted to unilateral ureteral obstruction (UUO), treated with bone-marrow mononuclear cells (UUO+BMMC) infused via the cava vein, and killed on day 14. Labeled BMMC were seen in renal tissue after 7 days in the group UUO+BMMC. UUO+BMMC also showed a reduction in ED1+ cells and tubular apoptotic cells together with enhanced tubular proliferation. Myofibroblasts were also reduced after BMMC which is consistent with a decrease in collagen deposition (picro Sirius staining) and RT-PCR data showing lower levels of procollagen-I mRNA. Simultaneously, nestin+ cells increased in the interstitium and decreased in the tubules. Double stained nestin+/α-SMA+ cells were present only in the interstitium, and their levels did not change after BMMC infusion. These data indicate a renoprotective effect of BMMC through increased tubular cell regeneration, inhibition of tubular cell apoptosis and partially blocking of the inflammatory and fibrotic events that occur after unilateral ureteral obstruction.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28369/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALbernardo_pascarelli_etal_IOC_2009.pdfbernardo_pascarelli_etal_IOC_2009.pdfapplication/pdf1400408https://www.arca.fiocruz.br/bitstream/icict/28369/2/bernardo_pascarelli_etal_IOC_2009.pdfb6c3349e4624fd5bbbced46f1d129e0cMD52TEXTbernardo_pascarelli_etal_IOC_2009.pdf.txtbernardo_pascarelli_etal_IOC_2009.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction |
| title |
Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction |
| spellingShingle |
Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction Barreira, André L. Nefropatias Células-Tronco Adultas Obstrução ureteral unilateral Reparação de tecidos Células mononucleares da medula óssea Bone marrow mononuclear cells Unilateral ureteral obstruction Tissue repair Nephropathies Adult stem cells |
| title_short |
Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction |
| title_full |
Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction |
| title_fullStr |
Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction |
| title_full_unstemmed |
Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction |
| title_sort |
Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction |
| author |
Barreira, André L. |
| author_facet |
Barreira, André L. Takiya, Christina M. Castiglione, Raquel C. Maron-Gutierrez, Tatiana Barbosa, Carolina M. L. Ornellas, Débora S. Verdoorn, Karine S. Pascarelli, Bernardo M. Borojevic, Radovan Einicker-Lamas, Marcelo Leite Jr., Maurilio Morales, Marcelo M. Vieyra, Adalberto |
| author_role |
author |
| author2 |
Takiya, Christina M. Castiglione, Raquel C. Maron-Gutierrez, Tatiana Barbosa, Carolina M. L. Ornellas, Débora S. Verdoorn, Karine S. Pascarelli, Bernardo M. Borojevic, Radovan Einicker-Lamas, Marcelo Leite Jr., Maurilio Morales, Marcelo M. Vieyra, Adalberto |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Barreira, André L. Takiya, Christina M. Castiglione, Raquel C. Maron-Gutierrez, Tatiana Barbosa, Carolina M. L. Ornellas, Débora S. Verdoorn, Karine S. Pascarelli, Bernardo M. Borojevic, Radovan Einicker-Lamas, Marcelo Leite Jr., Maurilio Morales, Marcelo M. Vieyra, Adalberto |
| dc.subject.other.pt_BR.fl_str_mv |
Nefropatias Células-Tronco Adultas Obstrução ureteral unilateral Reparação de tecidos Células mononucleares da medula óssea |
| topic |
Nefropatias Células-Tronco Adultas Obstrução ureteral unilateral Reparação de tecidos Células mononucleares da medula óssea Bone marrow mononuclear cells Unilateral ureteral obstruction Tissue repair Nephropathies Adult stem cells |
| dc.subject.en.pt_BR.fl_str_mv |
Bone marrow mononuclear cells Unilateral ureteral obstruction Tissue repair Nephropathies Adult stem cells |
| description |
Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2009 |
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2009 |
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2018-08-27T12:24:16Z |
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2018-08-27T12:24:16Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
BARREIRA, André L. et al. Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction. Cell Physiol Biochem., v. 24, p.585-594, 2009. |
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https://www.arca.fiocruz.br/handle/icict/28369 |
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1015-8987 |
| dc.identifier.eissn.none.fl_str_mv |
1421-9778 |
| identifier_str_mv |
BARREIRA, André L. et al. Bone Marrow Mononuclear Cells Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells after Unilateral Ureteral Obstruction. Cell Physiol Biochem., v. 24, p.585-594, 2009. 1015-8987 1421-9778 |
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Karger Publishers |
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Karger Publishers |
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