Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.

Grenfell, Rafaella Fortini Queiroz; Martins, Watson; Enk, Martin; Oliveira, Áureo Almeida de; Siqueira, Liliane; Moraes, Vanessa Silva; Oliveira, Edward José de; Carneiro, Nídia Francisca de Figueiredo; Coelho, Paulo Marcos Zech

Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.

Detalhes bibliográficos
Autor(a) principal:	Grenfell, Rafaella Fortini Queiroz
Data de Publicação:	2013
Outros Autores:	Martins, Watson, Enk, Martin, Oliveira, Áureo Almeida de, Siqueira, Liliane, Moraes, Vanessa Silva, Oliveira, Edward José de, Carneiro, Nídia Francisca de Figueiredo, Coelho, Paulo Marcos Zech
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/29891
Resumo:	Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil

Metadados do item

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spelling	Grenfell, Rafaella Fortini QueirozMartins, WatsonEnk, MartinOliveira, Áureo Almeida deSiqueira, LilianeMoraes, Vanessa SilvaOliveira, Edward José deCarneiro, Nídia Francisca de FigueiredoCoelho, Paulo Marcos Zech2018-11-06T13:25:42Z2018-11-06T13:25:42Z2013GRENFELL, Rafaella Fortini Queiroz et al. Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays. Mem. Inst. Oswaldo Cruz, v. 108, n. 3, p. 328-334, 20130074-0276https://www.arca.fiocruz.br/handle/icict/2989110.1590/S0074-02762013000300011engFundação Oswaldo CruzSchistosoma mansoniimunodiagnosticobaixa carga parasitáriaindivíduos da área endêmicadiagnostico adicionalSchistosoma mansoniimmunodiagnosislow parasite burdenendemic area individualsadditional diagnosisSchistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, BrasilPrefeitura de Montes Claros. Centro de Controle de Zoonoses. Montes Claros. MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilSchistosomiasis diagnosis is based on the detection of eggs in the faeces, which is laborious and lacks sensitivity, especially for patients with a low parasite burden. Immunological assays for specific antibody detection are available, but they usually demonstrate low sensitivity and/or specificity. In this study, two simple immunological assays were evaluated for the detection of soluble Schistosoma mansoni adult worm preparation (SWAP) and egg-specific IgGs. These studies have not yet been evaluated for patients with low parasite burdens. Residents of an endemic area in Brazil donated sera and faecal samples for our study. The patients were initially diagnosed by a rigorous Kato-Katz analysis of 18 thick smears from four different stool samples. The ELISA-SWAP was successful for human diagnosis with 90% sensitivity and specificity, confirming the Kato-Katz diagnosis with nearly perfect agreement, as seen by the Kappa index (0.85). Although the ELISA-soluble S. mansoni egg antigen was 85% sensitive, it exhibited low specificity (80%; Kappa index: 0.75) and was more susceptible to cross-reactivity. We believe that immunological assays should be used in conjunction with Kato-Katz analysis as a supplementary tool for the diagnosis of schistosomiasis for patients with low infection burdens, which are usually hard to detect.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/29891/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALSchistosoma mansoni in a low-prevalence.pdfSchistosoma mansoni in a low-prevalence.pdfapplication/pdf6911875https://www.arca.fiocruz.br/bitstream/icict/29891/2/Schistosoma%20mansoni%20in%20a%20low-prevalence.pdf24cf4dd22b5aedbafc58a46ea37b3f55MD52TEXTSchistosoma mansoni in a low-prevalence.pdf.txtSchistosoma mansoni in a low-prevalence.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv	Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.
title	Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.
spellingShingle	Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays. Grenfell, Rafaella Fortini Queiroz Schistosoma mansoni imunodiagnostico baixa carga parasitária indivíduos da área endêmica diagnostico adicional Schistosoma mansoni immunodiagnosis low parasite burden endemic area individuals additional diagnosis
title_short	Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.
title_full	Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.
title_fullStr	Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.
title_full_unstemmed	Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.
title_sort	Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.
author	Grenfell, Rafaella Fortini Queiroz
author_facet	Grenfell, Rafaella Fortini Queiroz Martins, Watson Enk, Martin Oliveira, Áureo Almeida de Siqueira, Liliane Moraes, Vanessa Silva Oliveira, Edward José de Carneiro, Nídia Francisca de Figueiredo Coelho, Paulo Marcos Zech
author_role	author
author2	Martins, Watson Enk, Martin Oliveira, Áureo Almeida de Siqueira, Liliane Moraes, Vanessa Silva Oliveira, Edward José de Carneiro, Nídia Francisca de Figueiredo Coelho, Paulo Marcos Zech
author2_role	author author author author author author author author
dc.contributor.author.fl_str_mv	Grenfell, Rafaella Fortini Queiroz Martins, Watson Enk, Martin Oliveira, Áureo Almeida de Siqueira, Liliane Moraes, Vanessa Silva Oliveira, Edward José de Carneiro, Nídia Francisca de Figueiredo Coelho, Paulo Marcos Zech
dc.subject.other.pt_BR.fl_str_mv	Schistosoma mansoni imunodiagnostico baixa carga parasitária indivíduos da área endêmica diagnostico adicional
topic	Schistosoma mansoni imunodiagnostico baixa carga parasitária indivíduos da área endêmica diagnostico adicional Schistosoma mansoni immunodiagnosis low parasite burden endemic area individuals additional diagnosis
dc.subject.en.pt_BR.fl_str_mv	Schistosoma mansoni immunodiagnosis low parasite burden endemic area individuals additional diagnosis
description	Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
publishDate	2013
dc.date.issued.fl_str_mv	2013
dc.date.accessioned.fl_str_mv	2018-11-06T13:25:42Z
dc.date.available.fl_str_mv	2018-11-06T13:25:42Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	GRENFELL, Rafaella Fortini Queiroz et al. Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays. Mem. Inst. Oswaldo Cruz, v. 108, n. 3, p. 328-334, 2013
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/29891
dc.identifier.issn.pt_BR.fl_str_mv	0074-0276
dc.identifier.doi.none.fl_str_mv	10.1590/S0074-02762013000300011
identifier_str_mv	GRENFELL, Rafaella Fortini Queiroz et al. Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays. Mem. Inst. Oswaldo Cruz, v. 108, n. 3, p. 328-334, 2013 0074-0276 10.1590/S0074-02762013000300011
url	https://www.arca.fiocruz.br/handle/icict/29891
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Fundação Oswaldo Cruz
publisher.none.fl_str_mv	Fundação Oswaldo Cruz
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays.

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