A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3390/v13122361 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50757 |
Resumo: | Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. |
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Lopes, Josiane Iole FrançaSilva, Carlos Alexandre da CostaCunha, Rodrigo GuimarãesSoares, Alexandra MartinsLopes, Maria Esther DuarteConceição Neto, Orlando Carlos daAlves, Arthur Daniel RochaCoelho, Wagner Luis da Costa Nunes PimentelAmorim Filho, LuizLeon, Luciane Almeida Amado2022-01-14T15:09:09Z2022-01-14T15:09:09Z2021LOPES, Josiane Iole França et al. A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders. Viruses, v. 13, 2361, p. 1-11, Nov. 2021.1999-4915https://www.arca.fiocruz.br/handle/icict/5075710.3390/v13122361engMDPISalivaSARS-CoV-2COVID-19DiagnósticoDoença hematológicaDistúrbios hemorrágicosSalivaSARS-CoV-2COVID-19DiagnosisHematological diseaseBleeding disordersA Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disordersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Diagnosis of SARS-CoV-2 infections is mostly based on the nasopharyngeal swabs (NPS). However, this collection is invasive and uncomfortable, especially for children and patients with coagulopathies, whose NPS collection often causes bleeding. Thus, the aim of this study was to evaluate the usefulness and accuracy of saliva for the diagnosis of COVID-19 in patients presenting bleeding disorders. Samples of NPS, oropharyngeal swabs (OPS), and saliva were collected simultaneously from 1159 hospitalized patients with hematological diseases and from 524 healthcare workers, both symptomatic and asymptomatic for SARS-CoV-2. All samples were evaluated for SARS-CoV-2 by qRT-PCR. SARS-CoV-2 was detected in NPS, OPS and saliva from 16.9%, 14.4% and 15.6% individuals, respectively. Tests in saliva showed sensitivity, specificity, and overall agreement of 73.3%, 96.9% and 92.7% (=0.74), respectively. Salivary tests had good accuracy (AUC = 0.7) for discriminating negative and positive qRT-PCR for SARS-CoV-2. Higher sensitivity was observed in symptomatic than in non-symptomatic patients, as well as in healthy subjects than in patients with hematological disease, in both OPS and saliva. The mean viral load in NPS was significantly higher than in OPS and in saliva samples (p < 0.001). Saliva is a good diagnostic tool to detect SARS-CoV-2, especially among patients symptomatic for COVID-19, and is a valuable specimen for mass screening of hospitalized patients with hematological diseases, especially for those that with bleeding disorders.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50757/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJosianeLopes_ArturAlves_etal_IOC_2021.pdfJosianeLopes_ArturAlves_etal_IOC_2021.pdfapplication/pdf2656215https://www.arca.fiocruz.br/bitstream/icict/50757/2/JosianeLopes_ArturAlves_etal_IOC_2021.pdf163f6f1cf32b4a00b2b1f07eac05c13eMD52icict/507572022-02-01 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| dc.title.pt_BR.fl_str_mv |
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders |
| title |
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders |
| spellingShingle |
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders Lopes, Josiane Iole França Saliva SARS-CoV-2 COVID-19 Diagnóstico Doença hematológica Distúrbios hemorrágicos Saliva SARS-CoV-2 COVID-19 Diagnosis Hematological disease Bleeding disorders |
| title_short |
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders |
| title_full |
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders |
| title_fullStr |
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders |
| title_full_unstemmed |
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders |
| title_sort |
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders |
| author |
Lopes, Josiane Iole França |
| author_facet |
Lopes, Josiane Iole França Silva, Carlos Alexandre da Costa Cunha, Rodrigo Guimarães Soares, Alexandra Martins Lopes, Maria Esther Duarte Conceição Neto, Orlando Carlos da Alves, Arthur Daniel Rocha Coelho, Wagner Luis da Costa Nunes Pimentel Amorim Filho, Luiz Leon, Luciane Almeida Amado |
| author_role |
author |
| author2 |
Silva, Carlos Alexandre da Costa Cunha, Rodrigo Guimarães Soares, Alexandra Martins Lopes, Maria Esther Duarte Conceição Neto, Orlando Carlos da Alves, Arthur Daniel Rocha Coelho, Wagner Luis da Costa Nunes Pimentel Amorim Filho, Luiz Leon, Luciane Almeida Amado |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lopes, Josiane Iole França Silva, Carlos Alexandre da Costa Cunha, Rodrigo Guimarães Soares, Alexandra Martins Lopes, Maria Esther Duarte Conceição Neto, Orlando Carlos da Alves, Arthur Daniel Rocha Coelho, Wagner Luis da Costa Nunes Pimentel Amorim Filho, Luiz Leon, Luciane Almeida Amado |
| dc.subject.other.pt_BR.fl_str_mv |
Saliva SARS-CoV-2 COVID-19 Diagnóstico Doença hematológica Distúrbios hemorrágicos |
| topic |
Saliva SARS-CoV-2 COVID-19 Diagnóstico Doença hematológica Distúrbios hemorrágicos Saliva SARS-CoV-2 COVID-19 Diagnosis Hematological disease Bleeding disorders |
| dc.subject.en.pt_BR.fl_str_mv |
Saliva SARS-CoV-2 COVID-19 Diagnosis Hematological disease Bleeding disorders |
| description |
Instituto de Hematologia Arthur de Siqueira Cavalcanti/Hemorio.Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021 |
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2022-01-14T15:09:09Z |
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2022-01-14T15:09:09Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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LOPES, Josiane Iole França et al. A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders. Viruses, v. 13, 2361, p. 1-11, Nov. 2021. |
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https://www.arca.fiocruz.br/handle/icict/50757 |
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1999-4915 |
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10.3390/v13122361 |
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LOPES, Josiane Iole França et al. A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders. Viruses, v. 13, 2361, p. 1-11, Nov. 2021. 1999-4915 10.3390/v13122361 |
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