Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?

Detalhes bibliográficos
Autor(a) principal: Madeira, Fernanda Fernandez
Data de Publicação: 2021
Outros Autores: Delgado, Luiza Maria Grzyb, Bittinelli, Isadora de Freitas, Oliveira, Jader de, Ravazi, Amanda, Reis, Yago Visinho dos, Oliveira, Ana Beatriz Bortolozo de, Cristal, Daniel Cesaretto, Galvão, Cleber, Oliveira, Maria Tercília Vilela de Azeredo, Rosa, João Aristeu da, Alevi, Kaio Cesar Chaboli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/49632
Resumo: Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.
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spelling Madeira, Fernanda FernandezDelgado, Luiza Maria GrzybBittinelli, Isadora de FreitasOliveira, Jader deRavazi, AmandaReis, Yago Visinho dosOliveira, Ana Beatriz Bortolozo deCristal, Daniel CesarettoGalvão, CleberOliveira, Maria Tercília Vilela de AzeredoRosa, João Aristeu daAlevi, Kaio Cesar Chaboli2021-11-02T13:24:58Z2021-11-02T13:24:58Z2021MADEIRA, Fernanda Fernandez et al. Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?. Parasites & Vectors, v. 13, n. 553, p. 1-8, 27 Oct. 2021.1756-3305https://www.arca.fiocruz.br/handle/icict/4963210.1186/s13071-021-04988-91756-3305engBMCVetores da Doença de ChagasTriatomíneosCruzamentos experimentaisDistância genéticaChagas disease vectorTriatominesExperimental crossesGenetic distanceTriatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Botucatu, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Botucatu, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Ciências Farmacêuticas. Laboratório de Parasitologia. Araraquara, SP, Brasil / Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia. São Paulo, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Botucatu, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Botucatu, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Ciências Farmacêuticas. Laboratório de Parasitologia. Araraquara, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Ciências Farmacêuticas. Laboratório de Parasitologia. Araraquara, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Botucatu, SP, Brasil / Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Ciências Farmacêuticas. Laboratório de Parasitologia. Araraquara, SP, Brasil.Background: Triatoma sordida is one of the main Chagas disease vectors in Brazil. In addition to Brazil, this species has already been reported in Bolivia, Argentina, Paraguay, and Uruguay. It is hypothesized that the insects currently identifed as T. sordida are a species subcomplex formed by three cytotypes (T. sordida sensu stricto [s.s.], T. sordida La Paz, and T. sordida Argentina). With the recent description of T. rosai from the Argentinean specimens, it became necessary to assess the taxonomic status of T. sordida from La Paz, Bolivia, since it was suggested that it may represent a new species, which has taxonomic, evolutionary, and epidemiological implications. Based on the above, we carried out molecular and experimental crossover studies to assess the specifc status of T. sordida La Paz. Methods: To evaluate the pre- and postzygotic barriers between T. sordida La Paz and T. sordida s.s., experimental crosses and intercrosses between F1 hybrids and between F2 hybrids were conducted. In addition, cytogenetic analyses of the F1 and F2 hybrids were applied with an emphasis on the degree of pairing between the homeologous chromosomes, and morphological analyses of the male gonads were performed to evaluate the presence of gonadal dysgenesis. Lastly, the genetic distance between T. sordida La Paz and T. sordida s.s. was calculated for the CYTB, ND1, and ITS1 genes. Results: Regardless of the gene used, T. sordida La Paz showed low genetic distance compared to T. sordida s.s. (below 2%). Experimental crosses resulted in ofspring for both directions, demonstrating that there are no prezygotic barriers installed between these allopatric populations. Furthermore, postzygotic barriers were not observed either (since the F1×F1 and F2×F2 intercrosses resulted in viable ofspring). Morphological and cytogenetic analyses of the male gonads of the F1 and F2 ofspring demonstrated that the testes were not atrophied and did not show chromosome pairing errors. Conclusion: Based on the low genetic distance (which confgures intraspecifc variation), associated with the absence of prezygotic and postzygotic reproductive barriers, we confrm that T. sordida La Paz represents only a chromosomal polymorphism of T. sordida s.s.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/49632/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCleber_Galvao_etal_IOC_2021.pdfCleber_Galvao_etal_IOC_2021.pdfapplication/pdf1079412https://www.arca.fiocruz.br/bitstream/icict/49632/2/Cleber_Galvao_etal_IOC_2021.pdfe880c0f907f526a0a7eb546ed5a0189bMD52icict/496322022-06-24 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dc.title.en.fl_str_mv Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?
title Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?
spellingShingle Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?
Madeira, Fernanda Fernandez
Vetores da Doença de Chagas
Triatomíneos
Cruzamentos experimentais
Distância genética
Chagas disease vector
Triatomines
Experimental crosses
Genetic distance
title_short Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?
title_full Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?
title_fullStr Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?
title_full_unstemmed Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?
title_sort Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?
author Madeira, Fernanda Fernandez
author_facet Madeira, Fernanda Fernandez
Delgado, Luiza Maria Grzyb
Bittinelli, Isadora de Freitas
Oliveira, Jader de
Ravazi, Amanda
Reis, Yago Visinho dos
Oliveira, Ana Beatriz Bortolozo de
Cristal, Daniel Cesaretto
Galvão, Cleber
Oliveira, Maria Tercília Vilela de Azeredo
Rosa, João Aristeu da
Alevi, Kaio Cesar Chaboli
author_role author
author2 Delgado, Luiza Maria Grzyb
Bittinelli, Isadora de Freitas
Oliveira, Jader de
Ravazi, Amanda
Reis, Yago Visinho dos
Oliveira, Ana Beatriz Bortolozo de
Cristal, Daniel Cesaretto
Galvão, Cleber
Oliveira, Maria Tercília Vilela de Azeredo
Rosa, João Aristeu da
Alevi, Kaio Cesar Chaboli
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Madeira, Fernanda Fernandez
Delgado, Luiza Maria Grzyb
Bittinelli, Isadora de Freitas
Oliveira, Jader de
Ravazi, Amanda
Reis, Yago Visinho dos
Oliveira, Ana Beatriz Bortolozo de
Cristal, Daniel Cesaretto
Galvão, Cleber
Oliveira, Maria Tercília Vilela de Azeredo
Rosa, João Aristeu da
Alevi, Kaio Cesar Chaboli
dc.subject.other.pt_BR.fl_str_mv Vetores da Doença de Chagas
Triatomíneos
Cruzamentos experimentais
Distância genética
topic Vetores da Doença de Chagas
Triatomíneos
Cruzamentos experimentais
Distância genética
Chagas disease vector
Triatomines
Experimental crosses
Genetic distance
dc.subject.en.en.fl_str_mv Chagas disease vector
Triatomines
Experimental crosses
Genetic distance
description Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-02T13:24:58Z
dc.date.available.fl_str_mv 2021-11-02T13:24:58Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MADEIRA, Fernanda Fernandez et al. Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?. Parasites & Vectors, v. 13, n. 553, p. 1-8, 27 Oct. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49632
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s13071-021-04988-9
dc.identifier.eissn.pt_BR.fl_str_mv 1756-3305
identifier_str_mv MADEIRA, Fernanda Fernandez et al. Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecifc chromosomal polymorphism?. Parasites & Vectors, v. 13, n. 553, p. 1-8, 27 Oct. 2021.
1756-3305
10.1186/s13071-021-04988-9
url https://www.arca.fiocruz.br/handle/icict/49632
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/49632/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/49632/2/Cleber_Galvao_etal_IOC_2021.pdf
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