Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique

Detalhes bibliográficos
Autor(a) principal: Mabunda, Nédio
Data de Publicação: 2022
Outros Autores: Augusto, Orvalho, Zicai, Ana Flora, Duajá, Ana, Oficiano, Sandra, Ismael, Nalia, Vubil, Adolfo, Mussá, Tufária, Moraes, Milton, Jani, Ilesh
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54586
Resumo: Instituto Nacional de Saúde, Marracuene, Mozambique / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
id CRUZ_f95cb1db98cb3777b74c6c525722c504
oai_identifier_str oai:www.arca.fiocruz.br:icict/54586
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Mabunda, NédioAugusto, OrvalhoZicai, Ana FloraDuajá, AnaOficiano, SandraIsmael, NaliaVubil, AdolfoMussá, TufáriaMoraes, MiltonJani, Ilesh2022-08-11T12:03:10Z2022-08-11T12:03:10Z2022MABUNDA, Nédio et al. Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique. Plos One, v. 17, n. 4, e0267472, p. 1 - 12, Apr. 2022.1932-6203https://www.arca.fiocruz.br/handle/icict/5458610.1371/journal.pone.0267472engPublic Library of ScienceTeste de ácido nucleicoIndicaçãoAlta prevalênciaVírus transmitidos pelo sangueDoadores de sangueMoçambiqueNucleic acid testingHigh prevalenceBlood borne virusesBlood donorsMozambiqueNucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambiqueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Saúde, Marracuene, Mozambique / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade Eduardo Mondlane. Faculdade de Medicina. Maputo, Mozambique.Instituto Nacional de Saúde. Marracuene, Mozambique.Instituto Nacional de Saúde. Marracuene, Mozambique / Hospital Central da Beira, Sofala, Mozambique.Hospital Central de Maputo. Maputo, Mozambique.Instituto Nacional de Saúde, Marracuene, Mozambique.Instituto Nacional de Saúde, Marracuene, Mozambique.Universidade Eduardo Mondlane. Faculdade de Medicina. Maputo, Mozambique.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Saúde, Marracuene, Mozambique.Background Although blood transfusion is an intervention that saves lives, it poses significant risks to the blood receivers, including the transmission of bloodborne pathogens. We aimed at determining the prevalence of Human Immunodeficiency Virus (HIV), Hepatitis B virus (HBV), and Hepatitis C virus (HCV) in candidates approved for blood donation, and in samples considered to be negative in reference blood banks in Mozambique. Methods A cross-sectional study was performed between November 2014 and October 2015 in Maputo and Beira cities. Demographic information was obtained from all consenting blood donors using a structured questionnaire. Plasma samples were screened for HIVAb/Ag combinations, HBsAg and Anti-HCV. Blood donors considered to be negative by serological testing were re-tested in pools of six plasma samples using nucleic acid testing (NAT). Results Most blood donors were male 2,320 (83.4%) with an age range of 18 to 34 years. The overall seroprevalence of HIV, HBV and HCV infections among blood donors approved for donation was 4.6% (127; 95% CI 3.8–5.4), 4.5% (124; 95% CI 3.7–5.3) and 0.4% (11; 95% CI 0.2– 0.7), respectively. The overall frequency by NAT of HIV RNA, HBV DNA, and HCV RNA in serologically negative blood donor samples was 2.6 per 1000 blood donors (7; 95% CI 1.1– 5.4); 12.5 per 1000 blood donors (33; 95% CI 8.6–17.5) and 2.6 per 1000 blood donors (6; 95% CI 1.0–5.7), respectively. Conclusion Our results show high seroprevalence of HIV and HBV infections in blood donors approved for donation, and high frequency of molecular biomarkers of HIV, HBV, and HCV in blood considered to be safe. These results suggest the need for a new blood screening policy in Mozambique, including the use of NAT to detect infectious blood donations during the immunologically negative window.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/54586/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMiltonMoraes_NedioMabunda_etal_IOC_2022.pdfMiltonMoraes_NedioMabunda_etal_IOC_2022.pdfapplication/pdf645077https://www.arca.fiocruz.br/bitstream/icict/54586/2/MiltonMoraes_NedioMabunda_etal_IOC_2022.pdf9f390a27c7e066784ac2070c211399eaMD52icict/545862022-08-11 09:03:10.843oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-08-11T12:03:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique
title Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique
spellingShingle Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique
Mabunda, Nédio
Teste de ácido nucleico
Indicação
Alta prevalência
Vírus transmitidos pelo sangue
Doadores de sangue
Moçambique
Nucleic acid testing
High prevalence
Blood borne viruses
Blood donors
Mozambique
title_short Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique
title_full Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique
title_fullStr Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique
title_full_unstemmed Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique
title_sort Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique
author Mabunda, Nédio
author_facet Mabunda, Nédio
Augusto, Orvalho
Zicai, Ana Flora
Duajá, Ana
Oficiano, Sandra
Ismael, Nalia
Vubil, Adolfo
Mussá, Tufária
Moraes, Milton
Jani, Ilesh
author_role author
author2 Augusto, Orvalho
Zicai, Ana Flora
Duajá, Ana
Oficiano, Sandra
Ismael, Nalia
Vubil, Adolfo
Mussá, Tufária
Moraes, Milton
Jani, Ilesh
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mabunda, Nédio
Augusto, Orvalho
Zicai, Ana Flora
Duajá, Ana
Oficiano, Sandra
Ismael, Nalia
Vubil, Adolfo
Mussá, Tufária
Moraes, Milton
Jani, Ilesh
dc.subject.other.pt_BR.fl_str_mv Teste de ácido nucleico
Indicação
Alta prevalência
Vírus transmitidos pelo sangue
Doadores de sangue
Moçambique
topic Teste de ácido nucleico
Indicação
Alta prevalência
Vírus transmitidos pelo sangue
Doadores de sangue
Moçambique
Nucleic acid testing
High prevalence
Blood borne viruses
Blood donors
Mozambique
dc.subject.en.pt_BR.fl_str_mv Nucleic acid testing
High prevalence
Blood borne viruses
Blood donors
Mozambique
description Instituto Nacional de Saúde, Marracuene, Mozambique / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-08-11T12:03:10Z
dc.date.available.fl_str_mv 2022-08-11T12:03:10Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MABUNDA, Nédio et al. Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique. Plos One, v. 17, n. 4, e0267472, p. 1 - 12, Apr. 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54586
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0267472
identifier_str_mv MABUNDA, Nédio et al. Nucleic acid testing identifies high prevalence of blood borne viruses among approved blood donors in Mozambique. Plos One, v. 17, n. 4, e0267472, p. 1 - 12, Apr. 2022.
1932-6203
10.1371/journal.pone.0267472
url https://www.arca.fiocruz.br/handle/icict/54586
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54586/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54586/2/MiltonMoraes_NedioMabunda_etal_IOC_2022.pdf
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
9f390a27c7e066784ac2070c211399ea
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009294985003008

Registros relacionados