Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts

Detalhes bibliográficos
Autor(a) principal: Pinto, Kamila Guimarães
Data de Publicação: 2018
Outros Autores: Nascimento, Danielle Oliveira, Ferreira, Antonia Corrêa, Morrot, Alexandre, Lima, Celio G. Freire de, Lopes, Marcela F., Reis, George A. dos, Filardy, Alessandra A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31426
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
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spelling Pinto, Kamila GuimarãesNascimento, Danielle OliveiraFerreira, Antonia CorrêaMorrot, AlexandreLima, Celio G. Freire deLopes, Marcela F.Reis, George A. dosFilardy, Alessandra A.2019-02-05T14:36:28Z2019-02-05T14:36:28Z2018PINTO, Kamila Guimarães et al. Trypanosoma cruzi infection induces cellular stress response and senescence-like Phenotype in Murine Fibroblasts. Frontiers in Immunology, v. 9, p. 1-11, July 2018.1664-3224https://www.arca.fiocruz.br/handle/icict/3142610.3389/fimmu.2018.01569engFrontiers MediaDoença de ChagasTrypanosoma cruziSenescênciaEspécies Reativas de Oxigênioβ-galactosidase associada à senescênciaFenótipo secretor associado à SenescênciaDeferoxamineChagas DiseaseTrypanosoma cruziSenescent-likeSenescence-associated β-galactosidaseSenescence-associated secretory phenotypeReactive oxygen speciesDeferoxamineEspécies Reativas de OxigênioTrypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblastsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Instituto Nacional para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica. Conselho Nacional de Desenvolvimento Científico e Tecnológico. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Trypanosoma cruzi infects and replicates within a wide variety of immune and non-immune cells. Here, we investigated early cellular responses induced in NIH-3T3 fibroblasts upon infection with trypomastigote forms of T. cruzi. We show that fibroblasts were susceptible to T. cruzi infection and started to release trypomastigotes to the culture medium after 4 days of infection. Also, we found that T. cruzi infection reduced the number of fibroblasts in 3-day cell cultures, by altering fibroblast proliferation. Infected fibroblasts displayed distinctive phenotypic alterations, including enlarged and flattened morphology with a nuclei accumulation of senescence-associated heterochromatin foci. In addition, infection induced an overexpression of the enzyme senescence-associated β-galactosidase (SA-β-gal), an activation marker of the cellular senescence program, as well as the production of cytokines and chemokines involved with the senescence-associated secretory phenotype (SASP) such as IL-6, TNF-α, IL-1β, and MCP-1. Infected fibroblasts released increased amounts of stress-associated factors nitric oxide (NO) and reactive oxygen species (ROS), and the treatment with antioxidants deferoxamine (DFO) and N-acetylcysteine reduced ROS generation, secretion of SASP-related cytokine IL-6, SA-β-gal activity, and parasite load by infected fibroblasts. Taken together, our data suggest that T. cruzi infection triggers a rapid cellular stress response followed by induction of a senescent-like phenotype in NIH-3T3 fibroblasts, enabling them to act as reservoirs of parasites during the early stages of the Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31426/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALalexandre2_morrot_etal_IOC_2018.pdfalexandre2_morrot_etal_IOC_2018.pdfapplication/pdf2547165https://www.arca.fiocruz.br/bitstream/icict/31426/2/alexandre2_morrot_etal_IOC_2018.pdf3c339420d50f9918dcac548b2244b769MD52TEXTalexandre2_morrot_etal_IOC_2018.pdf.txtalexandre2_morrot_etal_IOC_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts
title Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts
spellingShingle Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts
Pinto, Kamila Guimarães
Doença de Chagas
Trypanosoma cruzi
Senescência
Espécies Reativas de Oxigênio
β-galactosidase associada à senescência
Fenótipo secretor associado à Senescência
Deferoxamine
Chagas Disease
Trypanosoma cruzi
Senescent-like
Senescence-associated β-galactosidase
Senescence-associated secretory phenotype
Reactive oxygen species
Deferoxamine
Espécies Reativas de Oxigênio
title_short Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts
title_full Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts
title_fullStr Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts
title_full_unstemmed Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts
title_sort Trypanosoma cruzi Infection Induces Cellular Stress Response and Senescence-Like Phenotype in Murine Fibroblasts
author Pinto, Kamila Guimarães
author_facet Pinto, Kamila Guimarães
Nascimento, Danielle Oliveira
Ferreira, Antonia Corrêa
Morrot, Alexandre
Lima, Celio G. Freire de
Lopes, Marcela F.
Reis, George A. dos
Filardy, Alessandra A.
author_role author
author2 Nascimento, Danielle Oliveira
Ferreira, Antonia Corrêa
Morrot, Alexandre
Lima, Celio G. Freire de
Lopes, Marcela F.
Reis, George A. dos
Filardy, Alessandra A.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinto, Kamila Guimarães
Nascimento, Danielle Oliveira
Ferreira, Antonia Corrêa
Morrot, Alexandre
Lima, Celio G. Freire de
Lopes, Marcela F.
Reis, George A. dos
Filardy, Alessandra A.
dc.subject.other.pt_BR.fl_str_mv Doença de Chagas
Trypanosoma cruzi
Senescência
Espécies Reativas de Oxigênio
β-galactosidase associada à senescência
Fenótipo secretor associado à Senescência
Deferoxamine
topic Doença de Chagas
Trypanosoma cruzi
Senescência
Espécies Reativas de Oxigênio
β-galactosidase associada à senescência
Fenótipo secretor associado à Senescência
Deferoxamine
Chagas Disease
Trypanosoma cruzi
Senescent-like
Senescence-associated β-galactosidase
Senescence-associated secretory phenotype
Reactive oxygen species
Deferoxamine
Espécies Reativas de Oxigênio
dc.subject.en.pt_BR.fl_str_mv Chagas Disease
Trypanosoma cruzi
Senescent-like
Senescence-associated β-galactosidase
Senescence-associated secretory phenotype
Reactive oxygen species
Deferoxamine
dc.subject.decs.pt_BR.fl_str_mv Espécies Reativas de Oxigênio
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-05T14:36:28Z
dc.date.available.fl_str_mv 2019-02-05T14:36:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PINTO, Kamila Guimarães et al. Trypanosoma cruzi infection induces cellular stress response and senescence-like Phenotype in Murine Fibroblasts. Frontiers in Immunology, v. 9, p. 1-11, July 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31426
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2018.01569
identifier_str_mv PINTO, Kamila Guimarães et al. Trypanosoma cruzi infection induces cellular stress response and senescence-like Phenotype in Murine Fibroblasts. Frontiers in Immunology, v. 9, p. 1-11, July 2018.
1664-3224
10.3389/fimmu.2018.01569
url https://www.arca.fiocruz.br/handle/icict/31426
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31426/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31426/2/alexandre2_morrot_etal_IOC_2018.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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