Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44242 |
Resumo: | Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil, (Project: APQ 03097 15). |
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Cordeiro, Taps Aparecida ReisMartins, Helen RodriguesFranco, Diego LeoniSantos, Fred Luciano NevesCeledon, Paola Alejandra FioraniCantuaria, Vinicius LopesLana, Marta deReis, Alexandre BarbosaFerreira, Lucas Franco2020-10-28T12:53:03Z2020-10-28T12:53:03Z2020CORDEIRO, Taps Aparecida Reis et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors and Bioelectronics, 2020.0956-5663https://www.arca.fiocruz.br/handle/icict/4424210.1016/j.bios.2020.112573Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil, (Project: APQ 03097 15).In this work, a dual detection system based on an impedimetric immunosensor was developed for the lust time for the simultaneous detection of anti-Tgpanosoma cruzi and anti-Leishmania infanturn antibodies in human and dog serum samples. The IBMP 8.1 and rLcilA/rLci2B recombinant antigens were inunobilized over the surface of dual screen-printed carbon electrodes (W1 and W2) modified with poly (4-hydroxyphenylacetic acid). Under optimized conditions, the immunosensor recognized specific interactions for anti-T. cruzi antibodies up to a dilution of 1:10,240 and for anti-L. infant-tun up to 1:5120 in canine seams samples. Relative standard deviation (RSD) values of 2.8% for W1 and 3.6% for W2 were obtained for T. cruzi (W1) and L infannun antigen (W2) samples in three different electrodes for 3 days (n = 9). The immunosensor was stored at 4 °C for 8 weeks, with activity retention of 70.2% in W1 and 78.2% in W2. The results using the recombinant proteins revealed that all antigens discriminated between negative and positive samples (p < 0.0001) in both dog and human groups, as well as no cross-reactivity could be detected among sera with other infections. With this approach, immunosensor-based diagnostic tests achieved 100% accuracy, suggesting that the antigens are eligible to enter Phase-II studies.engElsevierChagasLeishmaniose visceralDetecção duplaImunossensor impedimétricoEletropolimerizaçãoChagasVisceral leishmaniasisDual detectionImpedimetric immunosensorElectropolymerizationImpedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/44242/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALCordeiro, T.A.R. Impedimetric immunosensor....pdfCordeiro, T.A.R. Impedimetric immunosensor....pdfapplication/pdf5200482https://www.arca.fiocruz.br/bitstream/icict/44242/2/Cordeiro%2c%20T.A.R.%20Impedimetric%20immunosensor....pdf0fa82fb7681d38814e282fca47cc5c80MD52TEXTCordeiro, T.A.R. Impedimetric immunosensor....pdf.txtCordeiro, T.A.R. 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| dc.title.pt_BR.fl_str_mv |
Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis |
| title |
Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis |
| spellingShingle |
Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis Cordeiro, Taps Aparecida Reis Chagas Leishmaniose visceral Detecção dupla Imunossensor impedimétrico Eletropolimerização Chagas Visceral leishmaniasis Dual detection Impedimetric immunosensor Electropolymerization |
| title_short |
Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis |
| title_full |
Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis |
| title_fullStr |
Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis |
| title_full_unstemmed |
Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis |
| title_sort |
Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis |
| author |
Cordeiro, Taps Aparecida Reis |
| author_facet |
Cordeiro, Taps Aparecida Reis Martins, Helen Rodrigues Franco, Diego Leoni Santos, Fred Luciano Neves Celedon, Paola Alejandra Fiorani Cantuaria, Vinicius Lopes Lana, Marta de Reis, Alexandre Barbosa Ferreira, Lucas Franco |
| author_role |
author |
| author2 |
Martins, Helen Rodrigues Franco, Diego Leoni Santos, Fred Luciano Neves Celedon, Paola Alejandra Fiorani Cantuaria, Vinicius Lopes Lana, Marta de Reis, Alexandre Barbosa Ferreira, Lucas Franco |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cordeiro, Taps Aparecida Reis Martins, Helen Rodrigues Franco, Diego Leoni Santos, Fred Luciano Neves Celedon, Paola Alejandra Fiorani Cantuaria, Vinicius Lopes Lana, Marta de Reis, Alexandre Barbosa Ferreira, Lucas Franco |
| dc.subject.other.pt_BR.fl_str_mv |
Chagas Leishmaniose visceral Detecção dupla Imunossensor impedimétrico Eletropolimerização |
| topic |
Chagas Leishmaniose visceral Detecção dupla Imunossensor impedimétrico Eletropolimerização Chagas Visceral leishmaniasis Dual detection Impedimetric immunosensor Electropolymerization |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas Visceral leishmaniasis Dual detection Impedimetric immunosensor Electropolymerization |
| description |
Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil, (Project: APQ 03097 15). |
| publishDate |
2020 |
| dc.date.accessioned.fl_str_mv |
2020-10-28T12:53:03Z |
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2020-10-28T12:53:03Z |
| dc.date.issued.fl_str_mv |
2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
CORDEIRO, Taps Aparecida Reis et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors and Bioelectronics, 2020. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/44242 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0956-5663 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.bios.2020.112573 |
| identifier_str_mv |
CORDEIRO, Taps Aparecida Reis et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors and Bioelectronics, 2020. 0956-5663 10.1016/j.bios.2020.112573 |
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https://www.arca.fiocruz.br/handle/icict/44242 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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