Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis

Detalhes bibliográficos
Autor(a) principal: Almeida-Silva, Fernando
Data de Publicação: 2023
Outros Autores: Almeida, Marcos de Abreu, Rabello, Vanessa Brito de Souza, Zancopé-Oliveira, Rosely Maria, Baeza, Lilian Cristiane, Lamas, Cristiane da Cruz, Lima, Marco Antonio, Macedo, Priscila Marques de, Gutierrez-Galhardo, Maria Clara, Almeida-Paes, Rodrigo, Freitas, Dayvison Francis Saraiva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59845
Resumo: This study was supported in part by the Programa Jovens Pesquisadores—INI/FIOCRUZ (grant INI-003-FIO-19-2-13) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (405653/2021-2 and 403296/2021-8). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. R.M.Z-O is supported in part by CNPq (308315/2021-9) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E-26/200.381/2023).
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spelling Almeida-Silva, FernandoAlmeida, Marcos de AbreuRabello, Vanessa Brito de SouzaZancopé-Oliveira, Rosely MariaBaeza, Lilian CristianeLamas, Cristiane da CruzLima, Marco AntonioMacedo, Priscila Marques deGutierrez-Galhardo, Maria ClaraAlmeida-Paes, RodrigoFreitas, Dayvison Francis Saraiva2023-07-31T01:15:00Z2023-07-31T01:15:00Z2023ALMEIDA-SILVA, Fernando et al. Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis. Journal of Fungi, v. 9, n. 5, p. 1-14, Apr. 2023.2309-608Xhttps://www.arca.fiocruz.br/handle/icict/5984510.3390/jof90505352309-608XThis study was supported in part by the Programa Jovens Pesquisadores—INI/FIOCRUZ (grant INI-003-FIO-19-2-13) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (405653/2021-2 and 403296/2021-8). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. R.M.Z-O is supported in part by CNPq (308315/2021-9) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E-26/200.381/2023).Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Oeste do Paraná. Centro de Ciências Médicas e Farmacêuticas. Cascavel, PR, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Serviço Médico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Sporotrichosis is the main subcutaneous mycosis worldwide. Several complications, including meningeal forms, can be observed in immunocompromised individuals. The sporotrichosis diagnosis is time-consuming due to the culture's limitations. The low fungal burden in cerebrospinal fluid (CSF) samples is another important drawback in the diagnosis of meningeal sporotrichosis. Molecular and immunological tests can improve the detection of Sporothrix spp. in clinical specimens. Therefore, the following five non-culture-based methods were evaluated for the detection of Sporothrix spp. in 30 CSF samples: (i) species-specific polymerase chain reaction (PCR); (ii) nested PCR; (iii) quantitative PCR; (iv) enzyme-linked immunosorbent assay (ELISA) for IgG detection; and (v) ELISA for IgM detection. The species-specific PCR was unsuccessful in the diagnosis of the meningeal sporotrichosis. The other four methods presented substantial levels of sensitivity (78.6% to 92.9%) and specificity (75% to 100%) for the indirect detection of Sporothrix spp. Both DNA-based methods presented similar accuracy (84.6%). Both ELISA methods were concomitantly positive only for patients with sporotrichosis and clinical signs of meningitis. We suggest that these methods should be implemented in clinical practice to detect Sporothrix spp. in CSF early, which may optimize treatment, augment the chances of a cure, and improve the prognosis of affected individuals.engMDPIEvaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleELISASporothrixCerebrospinal fluidImmunological diagnosisMolecular diagnosisqPCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis
title Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis
spellingShingle Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis
Almeida-Silva, Fernando
ELISA
Sporothrix
Cerebrospinal fluid
Immunological diagnosis
Molecular diagnosis
qPCR
title_short Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis
title_full Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis
title_fullStr Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis
title_full_unstemmed Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis
title_sort Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis
author Almeida-Silva, Fernando
author_facet Almeida-Silva, Fernando
Almeida, Marcos de Abreu
Rabello, Vanessa Brito de Souza
Zancopé-Oliveira, Rosely Maria
Baeza, Lilian Cristiane
Lamas, Cristiane da Cruz
Lima, Marco Antonio
Macedo, Priscila Marques de
Gutierrez-Galhardo, Maria Clara
Almeida-Paes, Rodrigo
Freitas, Dayvison Francis Saraiva
author_role author
author2 Almeida, Marcos de Abreu
Rabello, Vanessa Brito de Souza
Zancopé-Oliveira, Rosely Maria
Baeza, Lilian Cristiane
Lamas, Cristiane da Cruz
Lima, Marco Antonio
Macedo, Priscila Marques de
Gutierrez-Galhardo, Maria Clara
Almeida-Paes, Rodrigo
Freitas, Dayvison Francis Saraiva
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida-Silva, Fernando
Almeida, Marcos de Abreu
Rabello, Vanessa Brito de Souza
Zancopé-Oliveira, Rosely Maria
Baeza, Lilian Cristiane
Lamas, Cristiane da Cruz
Lima, Marco Antonio
Macedo, Priscila Marques de
Gutierrez-Galhardo, Maria Clara
Almeida-Paes, Rodrigo
Freitas, Dayvison Francis Saraiva
dc.subject.en.en_US.fl_str_mv ELISA
Sporothrix
Cerebrospinal fluid
Immunological diagnosis
Molecular diagnosis
qPCR
topic ELISA
Sporothrix
Cerebrospinal fluid
Immunological diagnosis
Molecular diagnosis
qPCR
description This study was supported in part by the Programa Jovens Pesquisadores—INI/FIOCRUZ (grant INI-003-FIO-19-2-13) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (405653/2021-2 and 403296/2021-8). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. R.M.Z-O is supported in part by CNPq (308315/2021-9) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E-26/200.381/2023).
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-07-31T01:15:00Z
dc.date.available.fl_str_mv 2023-07-31T01:15:00Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA-SILVA, Fernando et al. Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis. Journal of Fungi, v. 9, n. 5, p. 1-14, Apr. 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59845
dc.identifier.issn.en_US.fl_str_mv 2309-608X
dc.identifier.doi.none.fl_str_mv 10.3390/jof9050535
dc.identifier.eissn.none.fl_str_mv 2309-608X
identifier_str_mv ALMEIDA-SILVA, Fernando et al. Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis. Journal of Fungi, v. 9, n. 5, p. 1-14, Apr. 2023.
2309-608X
10.3390/jof9050535
url https://www.arca.fiocruz.br/handle/icict/59845
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59845/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/59845/2/ve_Fernando_Silva_etal_INI_2023.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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