Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells

Detalhes bibliográficos
Autor(a) principal: Stumbo, Ana Carolina
Data de Publicação: 2002
Outros Autores: Barbosa, Helene Santos, Carvalho, Técia Maria Ulisses de, Porto, Luís Cristóvão, Carvalho, Laís de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34343
Resumo: Universidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.
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spelling Stumbo, Ana CarolinaBarbosa, Helene SantosCarvalho, Técia Maria Ulisses dePorto, Luís CristóvãoCarvalho, Laís de2019-07-23T13:25:55Z2019-07-23T13:25:55Z2002STUMBO, Ana Carolina et al. Anionic Sites, Fucose Residues and Class I Human Leukocyte Antigen Fate During Interaction of Toxoplasma gondii with Endothelial Cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 4, p. 517-522, June 2002.0074-0276https://www.arca.fiocruz.br/handle/icict/3434310.1590/s0074-027620020004000121678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzToxoplasma gondiiCélulas EndoteliaisFerritinasAntígenos de Histocompatibilidade Classe IAntígenos de Histocompatibilidade Classe 1Toxoplasma gondiiEndothelial cellsFerritinUlex europaeus agglutinin-1Histocompatibility antigens Class ICélulas EndoteliaisAntígenos de Histocompatibilidade Classe IAntígenos de HistocompatibilidadeAnionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultra-estrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.Toxoplasma gondii invades and proliferates in human umbilical vein endothelial cells where it resides in a parasitophorous vacuole. In order to analyze which components of the endothelial cell plasma membrane are internalized and become part of the parasitophorous vacuole membrane, the culture of endothelial cells was labeled with cationized ferritin or UEA I lectin or anti Class I human leukocyte antigen (HLA) before or after infection with T. gondii. The results showed no cationized ferritin and UEA I lectin in any parasitophorous vacuole membrane, however, the Class I HLA molecule labeling was observed in some endocytic vacuoles containing parasite until 1 h of interaction with T. gondii. After 24 h parasite-host cell interaction, the labeling was absent on the vacuolar membrane, but presents only in small vesicles near parasitophorous vacuole. These results suggest the anionic site and fucose residues are excluded at the time of parasitophorous vacuole formation while Class I HLA molecules are present only on a minority of Toxoplasma-containing vacuoles.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34343/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALTeciaCarvalho_HeleneBarbosa_etal_IOC_2002.pdfTeciaCarvalho_HeleneBarbosa_etal_IOC_2002.pdfapplication/pdf542179https://www.arca.fiocruz.br/bitstream/icict/34343/2/TeciaCarvalho_HeleneBarbosa_etal_IOC_2002.pdf368f7a59ef26a297dc756990490fb554MD52TEXTTeciaCarvalho_HeleneBarbosa_etal_IOC_2002.pdf.txtTeciaCarvalho_HeleneBarbosa_etal_IOC_2002.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
title Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
spellingShingle Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
Stumbo, Ana Carolina
Toxoplasma gondii
Células Endoteliais
Ferritinas
Antígenos de Histocompatibilidade Classe I
Antígenos de Histocompatibilidade Classe 1
Toxoplasma gondii
Endothelial cells
Ferritin
Ulex europaeus agglutinin-1
Histocompatibility antigens Class I
Células Endoteliais
Antígenos de Histocompatibilidade Classe I
Antígenos de Histocompatibilidade
title_short Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
title_full Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
title_fullStr Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
title_full_unstemmed Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
title_sort Anionic sites, fucose residues and class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
author Stumbo, Ana Carolina
author_facet Stumbo, Ana Carolina
Barbosa, Helene Santos
Carvalho, Técia Maria Ulisses de
Porto, Luís Cristóvão
Carvalho, Laís de
author_role author
author2 Barbosa, Helene Santos
Carvalho, Técia Maria Ulisses de
Porto, Luís Cristóvão
Carvalho, Laís de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Stumbo, Ana Carolina
Barbosa, Helene Santos
Carvalho, Técia Maria Ulisses de
Porto, Luís Cristóvão
Carvalho, Laís de
dc.subject.other.pt_BR.fl_str_mv Toxoplasma gondii
Células Endoteliais
Ferritinas
Antígenos de Histocompatibilidade Classe I
Antígenos de Histocompatibilidade Classe 1
topic Toxoplasma gondii
Células Endoteliais
Ferritinas
Antígenos de Histocompatibilidade Classe I
Antígenos de Histocompatibilidade Classe 1
Toxoplasma gondii
Endothelial cells
Ferritin
Ulex europaeus agglutinin-1
Histocompatibility antigens Class I
Células Endoteliais
Antígenos de Histocompatibilidade Classe I
Antígenos de Histocompatibilidade
dc.subject.en.pt_BR.fl_str_mv Toxoplasma gondii
Endothelial cells
Ferritin
Ulex europaeus agglutinin-1
Histocompatibility antigens Class I
dc.subject.decs.pt_BR.fl_str_mv Células Endoteliais
Antígenos de Histocompatibilidade Classe I
Antígenos de Histocompatibilidade
description Universidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2019-07-23T13:25:55Z
dc.date.available.fl_str_mv 2019-07-23T13:25:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv STUMBO, Ana Carolina et al. Anionic Sites, Fucose Residues and Class I Human Leukocyte Antigen Fate During Interaction of Toxoplasma gondii with Endothelial Cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 4, p. 517-522, June 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34343
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762002000400012
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv STUMBO, Ana Carolina et al. Anionic Sites, Fucose Residues and Class I Human Leukocyte Antigen Fate During Interaction of Toxoplasma gondii with Endothelial Cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 4, p. 517-522, June 2002.
0074-0276
10.1590/s0074-02762002000400012
1678-8060
url https://www.arca.fiocruz.br/handle/icict/34343
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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