Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions

Detalhes bibliográficos
Autor(a) principal: Queiroz, Conceição
Data de Publicação: 2006
Outros Autores: Silva, Tânia Maria Correia, Alves, Venâncio Avancini Ferreira, Villa, Luisa L., Costa, Maria Cecília, Travassos, Ana Gabriela Álvares, Araújo Filho, José Bouzas, Studart, Eduardo, Cheto, Tatiana, Freitas, Luiz Antonio Rodrigues de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7867
Resumo: Federal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
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spelling Queiroz, ConceiçãoSilva, Tânia Maria CorreiaAlves, Venâncio Avancini FerreiraVilla, Luisa L.Costa, Maria CecíliaTravassos, Ana Gabriela ÁlvaresAraújo Filho, José BouzasStudart, EduardoCheto, TatianaFreitas, Luiz Antonio Rodrigues de2014-07-04T13:32:49Z2014-07-04T13:32:49Z2006QUEIROZ, C. et al. P16(INK4a) expression as a potential prognostic marker in cervical pre-neoplastic and neoplastic lesions. Pathology Research Practice, v. 202, n. 2, p. 731-737, 2006.0344-0338https://www.arca.fiocruz.br/handle/icict/786710.1016/j.prp.2006.07.003engElsevierComparative study of the expression of cellular cycle proteins in cervical intraepithelial lesionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilSão Paulo Federal University. Department of Pathology. Adolfo Lutz Institute. São Paulo, SP, BrasilLudwig Institute for Cancer Research. Virology Department. São Paulo, SP, BrasilLudwig Institute for Cancer Research. Virology Department. São Paulo, SP, BrasilFederal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, BrasilFederal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, BrasilFederal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilInteraction of human papilloma virus oncoproteins E6 and E7 with cell cycle proteins leads to disturbances of the cell cycle mechanism and subsequent alteration in the expression of some proteins, such as p16INK4a, cyclin D1, p53 and KI67. In this study, we compared alterations in the expression of these proteins during several stages of intraepitelial cervical carcinogenesis. Accordingly, an immunohistochemical study was performed on 50 cervical biopsies, including negative cases and intraepithelial neoplasias. The expression patterns of these markers were correlated with the histopathological diagnosis and infection with HPV. The p16INK4a, followed by Ki67, showed better correlation with cancer progression than p53 and cyclin D1, which recommends their use in the evaluation of cervical carcinogenesis. These monoclonal antibodies can be applied to cervical biopsy specimens to identify lesions transformed by oncogenic HPV, separating CIN 1 (p16INK4a positive) and identifying high-grade lesions by an increase in the cellular proliferation index (Ki67). In this way, we propose immunomarkers that can be applied in clinical practice to separate patients who need a conservative therapeutic approach from those who require a more aggressive treatment.Cervical neoplasiaP16INK4aCyclin D1Ki67p53Proteínas de Ciclo Celular/metabolismoNeoplasia Intraepitelial Cervical/metabolismoNeoplasias do Colo do Útero/metabolismoAdultoIdosoIdoso de 80 Anos ou maisBiópsiaProliferação de CélulasNeoplasia Intraepitelial Cervical/patologiaColo do Útero/anatomia & histologiaColo do Útero/metabolismoCiclina D1/metabolismoInibidor p16 de Quinase Ciclina-Dependente/metabolismoFemininoHumanosAntígeno Ki-67/metabolismoMeia-IdadeMarcadores Biológicos de Tumor/metabolismoNeoplasias do Colo do Útero/patologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALQueiroz C Comparative study ....pdfQueiroz C Comparative study ....pdfapplication/pdf170510https://www.arca.fiocruz.br/bitstream/icict/7867/1/Queiroz%20C%20Comparative%20study%20....pdf51870959887f49aeab5e1b9a7a7b0869MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7867/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTQueiroz C Comparative study ....pdf.txtQueiroz C Comparative study ....pdf.txtExtracted texttext/plain31312https://www.arca.fiocruz.br/bitstream/icict/7867/3/Queiroz%20C%20Comparative%20study%20....pdf.txtab170558886d322a3abfd76d856f37daMD53icict/78672023-03-15 14:33:15.358oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
title Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
spellingShingle Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
Queiroz, Conceição
Cervical neoplasia
P16INK4a
Cyclin D1
Ki67
p53
Proteínas de Ciclo Celular/metabolismo
Neoplasia Intraepitelial Cervical/metabolismo
Neoplasias do Colo do Útero/metabolismo
Adulto
Idoso
Idoso de 80 Anos ou mais
Biópsia
Proliferação de Células
Neoplasia Intraepitelial Cervical/patologia
Colo do Útero/anatomia & histologia
Colo do Útero/metabolismo
Ciclina D1/metabolismo
Inibidor p16 de Quinase Ciclina-Dependente/metabolismo
Feminino
Humanos
Antígeno Ki-67/metabolismo
Meia-Idade
Marcadores Biológicos de Tumor/metabolismo
Neoplasias do Colo do Útero/patologia
title_short Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
title_full Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
title_fullStr Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
title_full_unstemmed Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
title_sort Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
author Queiroz, Conceição
author_facet Queiroz, Conceição
Silva, Tânia Maria Correia
Alves, Venâncio Avancini Ferreira
Villa, Luisa L.
Costa, Maria Cecília
Travassos, Ana Gabriela Álvares
Araújo Filho, José Bouzas
Studart, Eduardo
Cheto, Tatiana
Freitas, Luiz Antonio Rodrigues de
author_role author
author2 Silva, Tânia Maria Correia
Alves, Venâncio Avancini Ferreira
Villa, Luisa L.
Costa, Maria Cecília
Travassos, Ana Gabriela Álvares
Araújo Filho, José Bouzas
Studart, Eduardo
Cheto, Tatiana
Freitas, Luiz Antonio Rodrigues de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Conceição
Silva, Tânia Maria Correia
Alves, Venâncio Avancini Ferreira
Villa, Luisa L.
Costa, Maria Cecília
Travassos, Ana Gabriela Álvares
Araújo Filho, José Bouzas
Studart, Eduardo
Cheto, Tatiana
Freitas, Luiz Antonio Rodrigues de
dc.subject.en.pt_BR.fl_str_mv Cervical neoplasia
P16INK4a
Cyclin D1
Ki67
p53
topic Cervical neoplasia
P16INK4a
Cyclin D1
Ki67
p53
Proteínas de Ciclo Celular/metabolismo
Neoplasia Intraepitelial Cervical/metabolismo
Neoplasias do Colo do Útero/metabolismo
Adulto
Idoso
Idoso de 80 Anos ou mais
Biópsia
Proliferação de Células
Neoplasia Intraepitelial Cervical/patologia
Colo do Útero/anatomia & histologia
Colo do Útero/metabolismo
Ciclina D1/metabolismo
Inibidor p16 de Quinase Ciclina-Dependente/metabolismo
Feminino
Humanos
Antígeno Ki-67/metabolismo
Meia-Idade
Marcadores Biológicos de Tumor/metabolismo
Neoplasias do Colo do Útero/patologia
dc.subject.decs.pt_BR.fl_str_mv Proteínas de Ciclo Celular/metabolismo
Neoplasia Intraepitelial Cervical/metabolismo
Neoplasias do Colo do Útero/metabolismo
Adulto
Idoso
Idoso de 80 Anos ou mais
Biópsia
Proliferação de Células
Neoplasia Intraepitelial Cervical/patologia
Colo do Útero/anatomia & histologia
Colo do Útero/metabolismo
Ciclina D1/metabolismo
Inibidor p16 de Quinase Ciclina-Dependente/metabolismo
Feminino
Humanos
Antígeno Ki-67/metabolismo
Meia-Idade
Marcadores Biológicos de Tumor/metabolismo
Neoplasias do Colo do Útero/patologia
description Federal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2014-07-04T13:32:49Z
dc.date.available.fl_str_mv 2014-07-04T13:32:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ, C. et al. P16(INK4a) expression as a potential prognostic marker in cervical pre-neoplastic and neoplastic lesions. Pathology Research Practice, v. 202, n. 2, p. 731-737, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7867
dc.identifier.issn.none.fl_str_mv 0344-0338
dc.identifier.doi.none.fl_str_mv 10.1016/j.prp.2006.07.003
identifier_str_mv QUEIROZ, C. et al. P16(INK4a) expression as a potential prognostic marker in cervical pre-neoplastic and neoplastic lesions. Pathology Research Practice, v. 202, n. 2, p. 731-737, 2006.
0344-0338
10.1016/j.prp.2006.07.003
url https://www.arca.fiocruz.br/handle/icict/7867
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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