A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging

Detalhes bibliográficos
Autor(a) principal: Silberstein, Erica
Data de Publicação: 2018
Outros Autores: Serna, Carylinda, Fragoso, Stenio Perdigão, Nagarkatti, Rana, Debrabant, Alain
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31952
Resumo: Laboratory of Emerging Pathogens. Center for Biologics Evaluation and Research. Food and Drug Administration. Maryland, United States of America.
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spelling Silberstein, EricaSerna, CarylindaFragoso, Stenio PerdigãoNagarkatti, RanaDebrabant, Alain2019-03-01T18:15:46Z2019-03-01T18:15:46Z2018SILBERSTEIN, Erica et al. A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging. PLoS ONE, v. 13, n. 4, 2018.1932-6203https://www.arca.fiocruz.br/handle/icict/3195210.1371/journal.pone.0195879porPublic Library of ScienceTrypanosoma cruziChagas DiseaseBioluminescence Resonance Energy Transfer TechniquesInfectionHost-Parasite InteractionsEnfermedad de ChagasTransferencia de Energía por Resonancia de BioluminiscenciaInfecciónInteracciones Huésped-ParásitosDoença de ChagasTécnicas de Transferência de Energia por Ressonância de BioluminescênciaInfecçãoInterações Hospedeiro-ParasitaA novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaginginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratory of Emerging Pathogens. Center for Biologics Evaluation and Research. Food and Drug Administration. Maryland, United States of America.Laboratory of Emerging Pathogens. Center for Biologics Evaluation and Research. Food and Drug Administration. Maryland, United States of America.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanossomatídeos. Curitiba, PR, Brasil.Laboratory of Emerging Pathogens. Center for Biologics Evaluation and Research. Food and Drug Administration. Maryland, United States of AmericaLaboratory of Emerging Pathogens. Center for Biologics Evaluation and Research. Food and Drug Administration. Maryland, United States of AmericaChagas disease, caused by the intracellular protozoan Trypanosoma cruzi, affects 8-10 million people worldwide and represents a major public health challenge. There is no effective treatment or vaccine to control the disease that is characterized by a mild acute phase followed by a chronic life-long infection. Approximately 30% of chronically infected individuals develop cardiac and/or digestive pathologies. T. cruzi can invade a wide variety of nucleated cells, but only persists at specific tissues in the host. However, the mechanisms that determine tissue tropism and the progression of the infection have not been fully described. Identification of infection niches in animal models has been difficult due to the limited quantity of parasite-infected cells and their focal distribution in tissues during the chronic phase. To better understand the course of chronic infections and parasite dissemination, we developed a bioluminescence imaging system based on the use of transgenic T. cruzi Colombiana strain parasites expressing nanoluciferase. Swiss Webster mice were infected with luminescent trypomastigotes and monitored for 126 days. Whole animal in vivo imaging showed parasites predominantly distributed in the abdominal cavity and surrounding areas throughout the infection. Bioluminescence signal reached a peak between 14 to 21 days post infection (dpi) and decreased progressively over time. Total animal luminescence could still be measured 126 dpi while parasites remained undetectable in blood by microscopy in most animals. Ex vivo imaging of specific tissues and organs dissected post-mortem at 126 dpi revealed a widespread parasite distribution in the skeletal muscle, heart, intestines and mesenteric fat. Parasites were also detected in lungs and liver. This noninvasive imaging model represents a novel tool to study host-parasite interactions and to identify parasite reservoirs of chronic Chagas Disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/31952/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALstenioPlosOn.pdfstenioPlosOn.pdfapplication/pdf30138879https://www.arca.fiocruz.br/bitstream/icict/31952/2/stenioPlosOn.pdf15a6f8f6376843092961f1211b003aaaMD52TEXTstenioPlosOn.pdf.txtstenioPlosOn.pdf.txtExtracted texttext/plain72005https://www.arca.fiocruz.br/bitstream/icict/31952/3/stenioPlosOn.pdf.txtb1150da5d1b66e2b5c531f38329748e1MD53icict/319522019-04-04 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dc.title.pt_BR.fl_str_mv A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
title A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
spellingShingle A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
Silberstein, Erica
Trypanosoma cruzi
Chagas Disease
Bioluminescence Resonance Energy Transfer Techniques
Infection
Host-Parasite Interactions
Enfermedad de Chagas
Transferencia de Energía por Resonancia de Bioluminiscencia
Infección
Interacciones Huésped-Parásitos
Doença de Chagas
Técnicas de Transferência de Energia por Ressonância de Bioluminescência
Infecção
Interações Hospedeiro-Parasita
title_short A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
title_full A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
title_fullStr A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
title_full_unstemmed A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
title_sort A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
author Silberstein, Erica
author_facet Silberstein, Erica
Serna, Carylinda
Fragoso, Stenio Perdigão
Nagarkatti, Rana
Debrabant, Alain
author_role author
author2 Serna, Carylinda
Fragoso, Stenio Perdigão
Nagarkatti, Rana
Debrabant, Alain
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silberstein, Erica
Serna, Carylinda
Fragoso, Stenio Perdigão
Nagarkatti, Rana
Debrabant, Alain
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
Chagas Disease
Bioluminescence Resonance Energy Transfer Techniques
Infection
Host-Parasite Interactions
Enfermedad de Chagas
Transferencia de Energía por Resonancia de Bioluminiscencia
Infección
Interacciones Huésped-Parásitos
Doença de Chagas
Técnicas de Transferência de Energia por Ressonância de Bioluminescência
Infecção
Interações Hospedeiro-Parasita
dc.subject.en.pt_BR.fl_str_mv Chagas Disease
Bioluminescence Resonance Energy Transfer Techniques
Infection
Host-Parasite Interactions
dc.subject.es.pt_BR.fl_str_mv Enfermedad de Chagas
Transferencia de Energía por Resonancia de Bioluminiscencia
Infección
Interacciones Huésped-Parásitos
dc.subject.decs.pt_BR.fl_str_mv Doença de Chagas
Técnicas de Transferência de Energia por Ressonância de Bioluminescência
Infecção
Interações Hospedeiro-Parasita
description Laboratory of Emerging Pathogens. Center for Biologics Evaluation and Research. Food and Drug Administration. Maryland, United States of America.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-03-01T18:15:46Z
dc.date.available.fl_str_mv 2019-03-01T18:15:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILBERSTEIN, Erica et al. A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging. PLoS ONE, v. 13, n. 4, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31952
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0195879
identifier_str_mv SILBERSTEIN, Erica et al. A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging. PLoS ONE, v. 13, n. 4, 2018.
1932-6203
10.1371/journal.pone.0195879
url https://www.arca.fiocruz.br/handle/icict/31952
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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