Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil

Detalhes bibliográficos
Autor(a) principal: Alves, Gleica Soyan Barbosa
Data de Publicação: 2016
Outros Autores: Freire, Ana Karla Lima, Bentes, Amaury dos Santos, Pinheiro, José Felipe de Souza, Souza, João Vicente Braga de, Wanke, Bodo, Matsuura, Takeshi, Jackisch-Matsuura, Ani Beatriz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32565
Resumo: 2020-04-15
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spelling Alves, Gleica Soyan BarbosaFreire, Ana Karla LimaBentes, Amaury dos SantosPinheiro, José Felipe de SouzaSouza, João Vicente Braga deWanke, BodoMatsuura, TakeshiJackisch-Matsuura, Ani Beatriz2019-04-17T21:21:33Z2019-04-17T21:21:33Z2016ALVES, Gleica Soyan Barbosa et al. Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil. Mycoses, v. 59, p. 509-515, 2016.0933-7407https://www.arca.fiocruz.br/handle/icict/3256510.1111/myc.124991439-0507engWileyMolecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-04-15Universidade Federal do Amazonas. Programa de Pós-Graduação em Saúde Sociedade e Endemias na Amazônia. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus, AM, Brasil.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil.Centro de Controle de Zoonoses de Manaus. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazonia. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Amazonas. Laboratório de Microbiologia. Manaus, AM, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus, AM, Brasil.Cryptococcus neoformans and Cryptococcus gattii are the main causative agents of cryptococcosis, a systemic fungal disease that affects internal organs and skin, and which is acquired by inhalation of spores or encapsulated yeasts. It is currently known that the C. neoformans/C. gattii species complex has a worldwide distribution, however, some molecular types seem to prevail in certain regions. Few environmental studies of Cryptococcus have been conducted in the Brazilian Amazon. This is the first ecological study of the pathogenic fungi C. neoformans/C. gattii species complex in the urban area of Manaus, Amazonas, Brazil. A total of 506 samples from pigeon droppings (n = 191), captive bird droppings (n = 60) and tree hollows (n = 255) were collected from June 2012 to January 2014 at schools and public buildings, squares, pet shops, households, the zoo and the bus station. Samples were plated on niger seed agar (NSA) medium supplemented with chloramphenicol and incubated at 25°C for 5 days. Dark-brown colonies were isolated and tested for thermotolerance at 37°C, cycloheximide resistance and growth on canavanine-glycine-bromothymol blue agar. Molecular typing was done by PCR-RFLP. Susceptibility to the antifungal drugs amphotericin B, fluconazole, itraconazole and ketoconazole was tested using Etest(®) strips. In total, 13 positive samples were obtained: one tree hollow (C. gattiiVGII), nine pigeon droppings (C. neoformansVNI) and three captive bird droppings (C. neoformansVNI). The environmental cryptococcal isolates found in this study were of the same molecular types as those responsible for infections in Manaus.Cryptococcus neoformansCryptococcus gattiiEnvironmental isolationGenotypingManausAmazonasBrazilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32565/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Alves_Gleica_etal_INI_2016.pdfve_Alves_Gleica_etal_INI_2016.pdfapplication/pdf193636https://www.arca.fiocruz.br/bitstream/icict/32565/2/ve_Alves_Gleica_etal_INI_2016.pdffa2a351dd1eca657476ccb1076bd9f72MD52TEXTve_Alves_Gleica_etal_INI_2016.pdf.txtve_Alves_Gleica_etal_INI_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
title Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
spellingShingle Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
Alves, Gleica Soyan Barbosa
Cryptococcus neoformans
Cryptococcus gattii
Environmental isolation
Genotyping
Manaus
Amazonas
Brazil
title_short Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
title_full Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
title_fullStr Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
title_full_unstemmed Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
title_sort Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil
author Alves, Gleica Soyan Barbosa
author_facet Alves, Gleica Soyan Barbosa
Freire, Ana Karla Lima
Bentes, Amaury dos Santos
Pinheiro, José Felipe de Souza
Souza, João Vicente Braga de
Wanke, Bodo
Matsuura, Takeshi
Jackisch-Matsuura, Ani Beatriz
author_role author
author2 Freire, Ana Karla Lima
Bentes, Amaury dos Santos
Pinheiro, José Felipe de Souza
Souza, João Vicente Braga de
Wanke, Bodo
Matsuura, Takeshi
Jackisch-Matsuura, Ani Beatriz
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Gleica Soyan Barbosa
Freire, Ana Karla Lima
Bentes, Amaury dos Santos
Pinheiro, José Felipe de Souza
Souza, João Vicente Braga de
Wanke, Bodo
Matsuura, Takeshi
Jackisch-Matsuura, Ani Beatriz
dc.subject.en.pt_BR.fl_str_mv Cryptococcus neoformans
Cryptococcus gattii
Environmental isolation
Genotyping
Manaus
Amazonas
Brazil
topic Cryptococcus neoformans
Cryptococcus gattii
Environmental isolation
Genotyping
Manaus
Amazonas
Brazil
description 2020-04-15
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-04-17T21:21:33Z
dc.date.available.fl_str_mv 2019-04-17T21:21:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVES, Gleica Soyan Barbosa et al. Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil. Mycoses, v. 59, p. 509-515, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32565
dc.identifier.issn.pt_BR.fl_str_mv 0933-7407
dc.identifier.doi.none.fl_str_mv 10.1111/myc.12499
dc.identifier.eissn.none.fl_str_mv 1439-0507
identifier_str_mv ALVES, Gleica Soyan Barbosa et al. Molecular typing of environmental Cryptococcus neoformans/C. gattii species complex isolates from Manaus, Amazonas, Brazil. Mycoses, v. 59, p. 509-515, 2016.
0933-7407
10.1111/myc.12499
1439-0507
url https://www.arca.fiocruz.br/handle/icict/32565
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/32565/2/ve_Alves_Gleica_etal_INI_2016.pdf
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