Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate

Detalhes bibliográficos
Autor(a) principal: Silva Filho, Fernando Costa e
Data de Publicação: 1987
Outros Autores: Elias, Cezar Antonio, Souza, Wanderley de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42884
Resumo: O processo de adesão de três cepas de Trichomonas vaginalis a um substrato de poliestireno foi estudado. Verificou-se que este processo depende do tempo de incubação e do pH da solução salina em que os parasitos se encontram. A maior taxa de adesão foi observada após 60 minutos de incubação a pH 6,6. A adesão é mior se Ca++ ou Mg++ for adicionado ao meio. Tratamento das células em citocalasina B, tripsina ou neuraminidase reduz a adesão enquanto tratamento com poli-L-lisina facilita esta adesão. Incubação dos parasitos ou esferas de poliestireno na presença de poli-L-lisina provoca alterações importantes na carga de superfície.
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spelling Silva Filho, Fernando Costa eElias, Cezar AntonioSouza, Wanderley de2020-08-22T17:45:12Z2020-08-22T17:45:12Z1987SILVA FILHO, Fernando Costa e; ELIAS, Cezar Antonio; SOUZA, Wanderley de. Role of divalent cations, pH, cytoskeleton componentes and surface charge on the adhesion of Trichomonas vaginalis to a polystyrene substrate. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 82, n. 3, p. 379-384, jul./set. 1987.0074-0276https://www.arca.fiocruz.br/handle/icict/4288410.1590/S0074-027619870003000091678-8060O processo de adesão de três cepas de Trichomonas vaginalis a um substrato de poliestireno foi estudado. Verificou-se que este processo depende do tempo de incubação e do pH da solução salina em que os parasitos se encontram. A maior taxa de adesão foi observada após 60 minutos de incubação a pH 6,6. A adesão é mior se Ca++ ou Mg++ for adicionado ao meio. Tratamento das células em citocalasina B, tripsina ou neuraminidase reduz a adesão enquanto tratamento com poli-L-lisina facilita esta adesão. Incubação dos parasitos ou esferas de poliestireno na presença de poli-L-lisina provoca alterações importantes na carga de superfície.he process of adhesion of three different strains of Trichomonas vaginalis to a polystyrene substrate was analysed. The process of adhesion was dependent on the time of incubation and the pH of the phosphate-buffered solution (PBS) in which the parasites were suspended. The highest indices of adhesion were observed after an incubation time of 60 min at pH 6.6. The adhesion index increased when the parasites were incubated in the presence of culture media or when Ca++ or Mg++ was added to the PBS solution, whereas cytochalasin B, trypsin or neuraminidase reduced adhesion. Incubation of the parasites in the presence of poly-L-lysine facilitated the process of adhesion. Incubation of the parasites or polystyrene beads in the presence of poly-L-lysine led to important changes in their surface charge.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trichomonas vaginalisAdesão celularComponentes do citoesqueletoCarga de superfícieTrichomonas vaginalisCell adhesioncytoskeletal componentsSurface chargeRole of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrateinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
title Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
spellingShingle Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
Silva Filho, Fernando Costa e
Trichomonas vaginalis
Adesão celular
Componentes do citoesqueleto
Carga de superfície
Trichomonas vaginalis
Cell adhesion
cytoskeletal components
Surface charge
title_short Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
title_full Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
title_fullStr Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
title_full_unstemmed Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
title_sort Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
author Silva Filho, Fernando Costa e
author_facet Silva Filho, Fernando Costa e
Elias, Cezar Antonio
Souza, Wanderley de
author_role author
author2 Elias, Cezar Antonio
Souza, Wanderley de
author2_role author
author
dc.contributor.author.fl_str_mv Silva Filho, Fernando Costa e
Elias, Cezar Antonio
Souza, Wanderley de
dc.subject.other.pt_BR.fl_str_mv Trichomonas vaginalis
Adesão celular
Componentes do citoesqueleto
Carga de superfície
topic Trichomonas vaginalis
Adesão celular
Componentes do citoesqueleto
Carga de superfície
Trichomonas vaginalis
Cell adhesion
cytoskeletal components
Surface charge
dc.subject.en.pt_BR.fl_str_mv Trichomonas vaginalis
Cell adhesion
cytoskeletal components
Surface charge
description O processo de adesão de três cepas de Trichomonas vaginalis a um substrato de poliestireno foi estudado. Verificou-se que este processo depende do tempo de incubação e do pH da solução salina em que os parasitos se encontram. A maior taxa de adesão foi observada após 60 minutos de incubação a pH 6,6. A adesão é mior se Ca++ ou Mg++ for adicionado ao meio. Tratamento das células em citocalasina B, tripsina ou neuraminidase reduz a adesão enquanto tratamento com poli-L-lisina facilita esta adesão. Incubação dos parasitos ou esferas de poliestireno na presença de poli-L-lisina provoca alterações importantes na carga de superfície.
publishDate 1987
dc.date.issued.fl_str_mv 1987
dc.date.accessioned.fl_str_mv 2020-08-22T17:45:12Z
dc.date.available.fl_str_mv 2020-08-22T17:45:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA FILHO, Fernando Costa e; ELIAS, Cezar Antonio; SOUZA, Wanderley de. Role of divalent cations, pH, cytoskeleton componentes and surface charge on the adhesion of Trichomonas vaginalis to a polystyrene substrate. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 82, n. 3, p. 379-384, jul./set. 1987.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42884
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761987000300009
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SILVA FILHO, Fernando Costa e; ELIAS, Cezar Antonio; SOUZA, Wanderley de. Role of divalent cations, pH, cytoskeleton componentes and surface charge on the adhesion of Trichomonas vaginalis to a polystyrene substrate. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 82, n. 3, p. 379-384, jul./set. 1987.
0074-0276
10.1590/S0074-02761987000300009
1678-8060
url https://www.arca.fiocruz.br/handle/icict/42884
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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