Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45651 |
Resumo: | Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. |
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Paes, Rodrigo AlmeidaAlmeida, Marcos AbreuBaeza, Lilian CristianeMarmello, Leticia Andrade MendesTrugilho, Monique Ramos de OliveiraNosanchuk, Joshua DanielSoares, Celia Maria de AlmeidaValente, Richard HemmiOliveira, Maria Zancopé2021-01-12T13:17:24Z2021-01-12T13:17:24Z2020PAES, Rodrigo Almeida et al. Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine. Fungi, v. 6, n. 328, p. 1-18, Dec. 2020.2309-608Xhttps://www.arca.fiocruz.br/handle/icict/4565110.3390/jof6040328engMDPIL-dihidroxyphenylalaninaMelaninaProteômicaVirulênciaMetabolismoParacoccidioidesParacoccidioidesMelaninL-dihydroxyphenylalanineProteomicsMetabolismVirulenceProteômicaBeyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Oeste do Paraná. Centro de Ciências Médicas e Farmacêuticas. Cascavel, PR, Brasil / Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Department of Medicine. Division of Infectious Diseases and Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, NY. USA.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil;Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Species of the genus Paracoccidioides cause a systemic infection in human patients. Yeast cells of Paracoccidioides spp. produce melanin in the presence of L-dihydroxyphenylalanine and during infection, which may impact the pathogen’s survival in the host. To better understand the metabolic changes that occur in melanized Paracoccidioides spp. cells, a proteomic approach was performed to compare melanized and non-melanized Paracoccidioides brasiliensis and Paracoccidioides lutzii yeast cells. Melanization was induced using L-dihydroxyphenylalanine as a precursor, and quantitative proteomics were performed using reversed-phase nano-chromatography coupled to high-resolution mass spectrometry. Whencomparing melanized versus non-melanized cells, 1006 and 582 di erentially abundant/detected proteins were identified for P. brasiliensis and P. lutzii, respectively. Functional enrichment and comparative analysis revealed 30 important KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways in melanized P. brasiliensis and 18 in P. lutzii, while di erentially abundant proteins from non-melanized cells from these species were involved in 21 and 25 enriched pathways, respectively. Melanized cells presented an abundance of additional virulence-associated proteins, such as phospholipase, proteases, superoxide dis-mutases, heat-shock proteins, adhesins, and proteins related to vesicular transport. The results suggest that L-dihydroxyphenylalanine increases the virulence of Paracoccidioides spp. through complex mechanisms involving not only melanin but other virulence factors as well.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45651/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMoniqueTrugillo_RichardValente_etal_IOC_2020.pdfMoniqueTrugillo_RichardValente_etal_IOC_2020.pdfapplication/pdf4624681https://www.arca.fiocruz.br/bitstream/icict/45651/2/MoniqueTrugillo_RichardValente_etal_IOC_2020.pdfe56d6067e920d3b7c20df01755c86ad0MD52TEXTMoniqueTrugillo_RichardValente_etal_IOC_2020.pdf.txtMoniqueTrugillo_RichardValente_etal_IOC_2020.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title |
Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| spellingShingle |
Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine Paes, Rodrigo Almeida L-dihidroxyphenylalanina Melanina Proteômica Virulência Metabolismo Paracoccidioides Paracoccidioides Melanin L-dihydroxyphenylalanine Proteomics Metabolism Virulence Proteômica |
| title_short |
Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_full |
Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_fullStr |
Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_full_unstemmed |
Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_sort |
Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| author |
Paes, Rodrigo Almeida |
| author_facet |
Paes, Rodrigo Almeida Almeida, Marcos Abreu Baeza, Lilian Cristiane Marmello, Leticia Andrade Mendes Trugilho, Monique Ramos de Oliveira Nosanchuk, Joshua Daniel Soares, Celia Maria de Almeida Valente, Richard Hemmi Oliveira, Maria Zancopé |
| author_role |
author |
| author2 |
Almeida, Marcos Abreu Baeza, Lilian Cristiane Marmello, Leticia Andrade Mendes Trugilho, Monique Ramos de Oliveira Nosanchuk, Joshua Daniel Soares, Celia Maria de Almeida Valente, Richard Hemmi Oliveira, Maria Zancopé |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Paes, Rodrigo Almeida Almeida, Marcos Abreu Baeza, Lilian Cristiane Marmello, Leticia Andrade Mendes Trugilho, Monique Ramos de Oliveira Nosanchuk, Joshua Daniel Soares, Celia Maria de Almeida Valente, Richard Hemmi Oliveira, Maria Zancopé |
| dc.subject.other.pt_BR.fl_str_mv |
L-dihidroxyphenylalanina Melanina Proteômica Virulência Metabolismo Paracoccidioides |
| topic |
L-dihidroxyphenylalanina Melanina Proteômica Virulência Metabolismo Paracoccidioides Paracoccidioides Melanin L-dihydroxyphenylalanine Proteomics Metabolism Virulence Proteômica |
| dc.subject.en.pt_BR.fl_str_mv |
Paracoccidioides Melanin L-dihydroxyphenylalanine Proteomics Metabolism Virulence |
| dc.subject.decs.pt_BR.fl_str_mv |
Proteômica |
| description |
Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020 |
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2021-01-12T13:17:24Z |
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2021-01-12T13:17:24Z |
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PAES, Rodrigo Almeida et al. Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine. Fungi, v. 6, n. 328, p. 1-18, Dec. 2020. |
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https://www.arca.fiocruz.br/handle/icict/45651 |
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2309-608X |
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10.3390/jof6040328 |
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PAES, Rodrigo Almeida et al. Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine. Fungi, v. 6, n. 328, p. 1-18, Dec. 2020. 2309-608X 10.3390/jof6040328 |
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