The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel

Detalhes bibliográficos
Autor(a) principal: Carvalho, Alex F.
Data de Publicação: 2021
Outros Autores: Rocha, Raissa P., Gonçalves, Andreza Parreiras, Silva, Thaís Barbara de Sousa, Sato, Hugo I., Vuitika, Larissa, Bagno, Flavia Fonseca, Sérgio, Sarah A. R., Figueiredo, Maria M., Martins, Ronaldo B., Souza, Juliano P., Arruda, Eurico, Fernandes, Ana Paula Salles Moura, Alves, Pedro Augusto, Teixeira, Santuza Maria Ribeiro, Fonseca, Flavio Guimarães da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51145
Resumo: Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brazil.
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spelling Carvalho, Alex F.Rocha, Raissa P.Gonçalves, Andreza ParreirasSilva, Thaís Barbara de SousaSato, Hugo I.Vuitika, LarissaBagno, Flavia FonsecaSérgio, Sarah A. R.Figueiredo, Maria M.Martins, Ronaldo B.Souza, Juliano P.Arruda, EuricoFernandes, Ana Paula Salles MouraAlves, Pedro AugustoTeixeira, Santuza Maria RibeiroFonseca, Flavio Guimarães da2022-02-10T18:53:32Z2022-02-10T18:53:32Z2021CARVALHO, Alex F. et al. The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel. Brazilian Journal of Microbiology, v. 52, n. 2, p. 531-539, 2021.1517-8382https://www.arca.fiocruz.br/handle/icict/5114510.1007/s42770-021-00469-4porSpringer International PublishingThe use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Virology Research Center. Department of Cell and Molecular Biology and Pathogenic Bioagents. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.Virology Research Center. Department of Cell and Molecular Biology and Pathogenic Bioagents. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.Virology Research Center. Department of Cell and Molecular Biology and Pathogenic Bioagents. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Accurate testing to detect SARS-CoV-2 RNA is key to counteract the virus spread. Nonetheless, the number of diagnostic laboratories able to perform qPCR tests is limited, particularly in developing countries. We describe the use of a virus-inactivating, denaturing solution (DS) to decrease virus infectivity in clinical specimens without affecting RNA integrity. Swab samples were collected from infected patients and from laboratory personnel using a commercially available viral transport solution and the in-house DS. Samples were tested by RT-qPCR, and exposure to infective viruses was also accessed by ELISA. The DS used did not interfere with viral genome detection and was able to maintain RNA integrity for up to 16 days at room temperature. Furthermore, virus loaded onto DS were inactivated, as attested by attempts to grow SARS-CoV-2 in cell monolayers after DS desalt filtration to remove toxic residues. The DS described here provides a strategy to maintain diagnostic accuracy and protects diagnostic laboratory personnel from accidental infection, as it has helped to protect our lab crew.COVID-19Laboratory personnelRNA integritySARS-CoV-2Sampling solutioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51145/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALCarvalho_Alex_etal_IRR_COVID-19_2021_ARTIGO_FINAL.pdfCarvalho_Alex_etal_IRR_COVID-19_2021_ARTIGO_FINAL.pdfapplication/pdf531745https://www.arca.fiocruz.br/bitstream/icict/51145/2/Carvalho_Alex_etal_IRR_COVID-19_2021_ARTIGO_FINAL.pdf612719b5658b171077cd46999eda6640MD52icict/511452022-11-13 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dc.title.pt_BR.fl_str_mv The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
title The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
spellingShingle The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
Carvalho, Alex F.
COVID-19
Laboratory personnel
RNA integrity
SARS-CoV-2
Sampling solution
title_short The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
title_full The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
title_fullStr The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
title_full_unstemmed The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
title_sort The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
author Carvalho, Alex F.
author_facet Carvalho, Alex F.
Rocha, Raissa P.
Gonçalves, Andreza Parreiras
Silva, Thaís Barbara de Sousa
Sato, Hugo I.
Vuitika, Larissa
Bagno, Flavia Fonseca
Sérgio, Sarah A. R.
Figueiredo, Maria M.
Martins, Ronaldo B.
Souza, Juliano P.
Arruda, Eurico
Fernandes, Ana Paula Salles Moura
Alves, Pedro Augusto
Teixeira, Santuza Maria Ribeiro
Fonseca, Flavio Guimarães da
author_role author
author2 Rocha, Raissa P.
Gonçalves, Andreza Parreiras
Silva, Thaís Barbara de Sousa
Sato, Hugo I.
Vuitika, Larissa
Bagno, Flavia Fonseca
Sérgio, Sarah A. R.
Figueiredo, Maria M.
Martins, Ronaldo B.
Souza, Juliano P.
Arruda, Eurico
Fernandes, Ana Paula Salles Moura
Alves, Pedro Augusto
Teixeira, Santuza Maria Ribeiro
Fonseca, Flavio Guimarães da
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Alex F.
Rocha, Raissa P.
Gonçalves, Andreza Parreiras
Silva, Thaís Barbara de Sousa
Sato, Hugo I.
Vuitika, Larissa
Bagno, Flavia Fonseca
Sérgio, Sarah A. R.
Figueiredo, Maria M.
Martins, Ronaldo B.
Souza, Juliano P.
Arruda, Eurico
Fernandes, Ana Paula Salles Moura
Alves, Pedro Augusto
Teixeira, Santuza Maria Ribeiro
Fonseca, Flavio Guimarães da
dc.subject.en.pt_BR.fl_str_mv COVID-19
Laboratory personnel
RNA integrity
SARS-CoV-2
Sampling solution
topic COVID-19
Laboratory personnel
RNA integrity
SARS-CoV-2
Sampling solution
description Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brazil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-02-10T18:53:32Z
dc.date.available.fl_str_mv 2022-02-10T18:53:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Alex F. et al. The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel. Brazilian Journal of Microbiology, v. 52, n. 2, p. 531-539, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51145
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.doi.pt_BR.fl_str_mv 10.1007/s42770-021-00469-4
identifier_str_mv CARVALHO, Alex F. et al. The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel. Brazilian Journal of Microbiology, v. 52, n. 2, p. 531-539, 2021.
1517-8382
10.1007/s42770-021-00469-4
url https://www.arca.fiocruz.br/handle/icict/51145
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Springer International Publishing
publisher.none.fl_str_mv Springer International Publishing
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bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51145/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51145/2/Carvalho_Alex_etal_IRR_COVID-19_2021_ARTIGO_FINAL.pdf
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