The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51145 |
Resumo: | Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brazil. |
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Carvalho, Alex F.Rocha, Raissa P.Gonçalves, Andreza ParreirasSilva, Thaís Barbara de SousaSato, Hugo I.Vuitika, LarissaBagno, Flavia FonsecaSérgio, Sarah A. R.Figueiredo, Maria M.Martins, Ronaldo B.Souza, Juliano P.Arruda, EuricoFernandes, Ana Paula Salles MouraAlves, Pedro AugustoTeixeira, Santuza Maria RibeiroFonseca, Flavio Guimarães da2022-02-10T18:53:32Z2022-02-10T18:53:32Z2021CARVALHO, Alex F. et al. The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel. Brazilian Journal of Microbiology, v. 52, n. 2, p. 531-539, 2021.1517-8382https://www.arca.fiocruz.br/handle/icict/5114510.1007/s42770-021-00469-4porSpringer International PublishingThe use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Virology Research Center. Department of Cell and Molecular Biology and Pathogenic Bioagents. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.Virology Research Center. Department of Cell and Molecular Biology and Pathogenic Bioagents. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.Virology Research Center. Department of Cell and Molecular Biology and Pathogenic Bioagents. Ribeirão Preto Medical School. University of São Paulo. Ribeirão Preto, SP, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brasil.Accurate testing to detect SARS-CoV-2 RNA is key to counteract the virus spread. Nonetheless, the number of diagnostic laboratories able to perform qPCR tests is limited, particularly in developing countries. We describe the use of a virus-inactivating, denaturing solution (DS) to decrease virus infectivity in clinical specimens without affecting RNA integrity. Swab samples were collected from infected patients and from laboratory personnel using a commercially available viral transport solution and the in-house DS. Samples were tested by RT-qPCR, and exposure to infective viruses was also accessed by ELISA. The DS used did not interfere with viral genome detection and was able to maintain RNA integrity for up to 16 days at room temperature. Furthermore, virus loaded onto DS were inactivated, as attested by attempts to grow SARS-CoV-2 in cell monolayers after DS desalt filtration to remove toxic residues. The DS described here provides a strategy to maintain diagnostic accuracy and protects diagnostic laboratory personnel from accidental infection, as it has helped to protect our lab crew.COVID-19Laboratory personnelRNA integritySARS-CoV-2Sampling solutioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51145/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALCarvalho_Alex_etal_IRR_COVID-19_2021_ARTIGO_FINAL.pdfCarvalho_Alex_etal_IRR_COVID-19_2021_ARTIGO_FINAL.pdfapplication/pdf531745https://www.arca.fiocruz.br/bitstream/icict/51145/2/Carvalho_Alex_etal_IRR_COVID-19_2021_ARTIGO_FINAL.pdf612719b5658b171077cd46999eda6640MD52icict/511452022-11-13 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| dc.title.pt_BR.fl_str_mv |
The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel |
| title |
The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel |
| spellingShingle |
The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel Carvalho, Alex F. COVID-19 Laboratory personnel RNA integrity SARS-CoV-2 Sampling solution |
| title_short |
The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel |
| title_full |
The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel |
| title_fullStr |
The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel |
| title_full_unstemmed |
The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel |
| title_sort |
The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel |
| author |
Carvalho, Alex F. |
| author_facet |
Carvalho, Alex F. Rocha, Raissa P. Gonçalves, Andreza Parreiras Silva, Thaís Barbara de Sousa Sato, Hugo I. Vuitika, Larissa Bagno, Flavia Fonseca Sérgio, Sarah A. R. Figueiredo, Maria M. Martins, Ronaldo B. Souza, Juliano P. Arruda, Eurico Fernandes, Ana Paula Salles Moura Alves, Pedro Augusto Teixeira, Santuza Maria Ribeiro Fonseca, Flavio Guimarães da |
| author_role |
author |
| author2 |
Rocha, Raissa P. Gonçalves, Andreza Parreiras Silva, Thaís Barbara de Sousa Sato, Hugo I. Vuitika, Larissa Bagno, Flavia Fonseca Sérgio, Sarah A. R. Figueiredo, Maria M. Martins, Ronaldo B. Souza, Juliano P. Arruda, Eurico Fernandes, Ana Paula Salles Moura Alves, Pedro Augusto Teixeira, Santuza Maria Ribeiro Fonseca, Flavio Guimarães da |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Carvalho, Alex F. Rocha, Raissa P. Gonçalves, Andreza Parreiras Silva, Thaís Barbara de Sousa Sato, Hugo I. Vuitika, Larissa Bagno, Flavia Fonseca Sérgio, Sarah A. R. Figueiredo, Maria M. Martins, Ronaldo B. Souza, Juliano P. Arruda, Eurico Fernandes, Ana Paula Salles Moura Alves, Pedro Augusto Teixeira, Santuza Maria Ribeiro Fonseca, Flavio Guimarães da |
| dc.subject.en.pt_BR.fl_str_mv |
COVID-19 Laboratory personnel RNA integrity SARS-CoV-2 Sampling solution |
| topic |
COVID-19 Laboratory personnel RNA integrity SARS-CoV-2 Sampling solution |
| description |
Universidade Federal de Minas Gerais. Centro de Tecnologia em Vacinas. Belo Horizonte, MG, Brazil. |
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2021 |
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2021 |
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2022-02-10T18:53:32Z |
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2022-02-10T18:53:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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CARVALHO, Alex F. et al. The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel. Brazilian Journal of Microbiology, v. 52, n. 2, p. 531-539, 2021. |
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https://www.arca.fiocruz.br/handle/icict/51145 |
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1517-8382 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1007/s42770-021-00469-4 |
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CARVALHO, Alex F. et al. The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel. Brazilian Journal of Microbiology, v. 52, n. 2, p. 531-539, 2021. 1517-8382 10.1007/s42770-021-00469-4 |
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Springer International Publishing |
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Springer International Publishing |
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