Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1128/EC.00148-10 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26166 |
| Resumo: | Este trabalho foi parcialmente financiado por subsídios fornecidos pelas agências de fomento brasileiras FACEPE ( 006/2003-CNPq / PPP ) e CNPq ( 476255 / 2004-0 ). O TDDCL, o DMNM e o CRSR receberam bolsas de estudo da CAPES e FACEPE. O JRCV recebeu uma bolsa PQ (“pesquisador visitante”) da FIOCRUZ. O trabalho no laboratório de M. Carrington é financiado pelo Wellcome Trust, e LE recebeu uma bolsa de estudos financiada pelo BBSRC. |
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Lima, Tamara D. da CostaMoura, Danielle M. N.Reis, Christian R. S.Vasconcelos, J. Ronnie C.Ellis, LouiseCarrington, MarkFigueiredo, Regina C. B. Q.Melo Neto, Osvaldo P. de2018-04-27T12:06:31Z2018-04-27T12:06:31Z2010DA COSTA LIMA, T. D. et al. Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners. Eukaryotic Cell, v. 9, n. 10, p. 1484–1494, out. 2010.1535-9786https://www.arca.fiocruz.br/handle/icict/2616610.1128/EC.00148-10Este trabalho foi parcialmente financiado por subsídios fornecidos pelas agências de fomento brasileiras FACEPE ( 006/2003-CNPq / PPP ) e CNPq ( 476255 / 2004-0 ). O TDDCL, o DMNM e o CRSR receberam bolsas de estudo da CAPES e FACEPE. O JRCV recebeu uma bolsa PQ (“pesquisador visitante”) da FIOCRUZ. O trabalho no laboratório de M. Carrington é financiado pelo Wellcome Trust, e LE recebeu uma bolsa de estudos financiada pelo BBSRC.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.University of Cambridge. Department of Biochemistry. Cambridge, United Kingdom.University of Cambridge. Department of Biochemistry. Cambridge, United Kingdom.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Trypanosomatid protozoans are reliant on posttranscriptional processes to control gene expression. Regulation occurs at the levels of mRNA processing, stability, and translation, events that may require the participation of the poly(A) binding protein (PABP). Here, we have undertaken a functional study of the three distinct Leishmania major PABP (LmPABP) homologues: the previously described LmPABP1; LmPABP2, orthologous to the PABP described from Trypanosoma species; and LmPABP3, unique to Leishmania. Sequence identity between the three PABPs is no greater than 40%. In assays measuring binding to A-rich sequences, LmPABP1 binding was poly(A) sensitive but heparin insensitive; LmPABP2 binding was heparin sensitive and less sensitive to poly(A), compatible with unique substitutions observed in residues implicated in poly(A) binding; and LmPABP3 displayed intermediate properties. All three homologues are simultaneously expressed as abundant cytoplasmic proteins in L. major promastigotes, but only LmPABP1 is present as multiple isoforms. Upon transcription inhibition, LmPABP2 and -3 migrated to the nucleus, while LmPABP1 remained predominantly cytoplasmic. Immunoprecipitation assays showed an association between LmPABP2 and -3. Although the three proteins bound to a Leishmania homologue of the translation initiation factor eukaryotic initiation factor 4G (eIF4G) (LmEIF4G3) in vitro, LmPABP1 was the only one to copurify with native LmEIF4G3 from cytoplasmic extracts. Functionality was tested using RNA interference (RNAi) in Trypanosoma brucei, where both orthologues to LmPABP1 and -2 are required for cellular viability. Our results indicate that these homologues have evolved divergent functions, some of which may be unique to the trypanosomatids, and reinforces a role for LmPABP1 in translation through its interaction with the eIF4G homologue.engLeishmaniaRNATrypanosoma bruceieIF4GLeishmaniaRNATrypanosoma bruceieIF4GSequência de AminoácidosAnimaisEncadernaçãoSobrevivência CelularFator de iniciação eucariótica-4G / metabolismoLeishmania major / genéticaLeishmania major / crescimento e desenvolvimentoLeishmania major / metabolismoDados de Sequencia MolecularPoly A / metabolismoProteínas de Ligação Poli (A) / químicaProteínas de Ligação Poli (A) / genéticaProteínas de Ligação Poli (A) / metabolismoLigação proteicaBiossíntese de ProteínasInterferência de RNARNA mensageiro / metabolismoHomologia de Sequência, AminoácidosTrypanosoma brucei brucei / genéticaTrypanosoma brucei brucei / metabolismoFunctional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partnersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFunctional Characterization of Three Leishmania Poly(A) Binding.pdfFunctional Characterization of Three Leishmania Poly(A) Binding.pdfapplication/pdf2122017https://www.arca.fiocruz.br/bitstream/icict/26166/2/Functional%20Characterization%20of%20Three%20Leishmania%20Poly%28A%29%20Binding.pdf3730cebc37c9949fa6814a700abd986cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26166/3/license.txt5a560609d32a3863062d77ff32785d58MD53TEXTFunctional Characterization of Three Leishmania Poly(A) Binding.pdf.txtFunctional Characterization of Three Leishmania Poly(A) Binding.pdf.txtExtracted texttext/plain65074https://www.arca.fiocruz.br/bitstream/icict/26166/4/Functional%20Characterization%20of%20Three%20Leishmania%20Poly%28A%29%20Binding.pdf.txt4af608dbddb0238d51dc4685e7074069MD54icict/261662018-08-15 03:05:53.713oai:www.arca.fiocruz.br:icict/26166Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-15T06:05:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners |
| title |
Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners |
| spellingShingle |
Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners Lima, Tamara D. da Costa Leishmania RNA Trypanosoma brucei eIF4G Leishmania RNA Trypanosoma brucei eIF4G Sequência de Aminoácidos Animais Encadernação Sobrevivência Celular Fator de iniciação eucariótica-4G / metabolismo Leishmania major / genética Leishmania major / crescimento e desenvolvimento Leishmania major / metabolismo Dados de Sequencia Molecular Poly A / metabolismo Proteínas de Ligação Poli (A) / química Proteínas de Ligação Poli (A) / genética Proteínas de Ligação Poli (A) / metabolismo Ligação proteica Biossíntese de Proteínas Interferência de RNA RNA mensageiro / metabolismo Homologia de Sequência, Aminoácidos Trypanosoma brucei brucei / genética Trypanosoma brucei brucei / metabolismo |
| title_short |
Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners |
| title_full |
Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners |
| title_fullStr |
Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners |
| title_full_unstemmed |
Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners |
| title_sort |
Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners |
| author |
Lima, Tamara D. da Costa |
| author_facet |
Lima, Tamara D. da Costa Moura, Danielle M. N. Reis, Christian R. S. Vasconcelos, J. Ronnie C. Ellis, Louise Carrington, Mark Figueiredo, Regina C. B. Q. Melo Neto, Osvaldo P. de |
| author_role |
author |
| author2 |
Moura, Danielle M. N. Reis, Christian R. S. Vasconcelos, J. Ronnie C. Ellis, Louise Carrington, Mark Figueiredo, Regina C. B. Q. Melo Neto, Osvaldo P. de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Tamara D. da Costa Moura, Danielle M. N. Reis, Christian R. S. Vasconcelos, J. Ronnie C. Ellis, Louise Carrington, Mark Figueiredo, Regina C. B. Q. Melo Neto, Osvaldo P. de |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania RNA Trypanosoma brucei eIF4G |
| topic |
Leishmania RNA Trypanosoma brucei eIF4G Leishmania RNA Trypanosoma brucei eIF4G Sequência de Aminoácidos Animais Encadernação Sobrevivência Celular Fator de iniciação eucariótica-4G / metabolismo Leishmania major / genética Leishmania major / crescimento e desenvolvimento Leishmania major / metabolismo Dados de Sequencia Molecular Poly A / metabolismo Proteínas de Ligação Poli (A) / química Proteínas de Ligação Poli (A) / genética Proteínas de Ligação Poli (A) / metabolismo Ligação proteica Biossíntese de Proteínas Interferência de RNA RNA mensageiro / metabolismo Homologia de Sequência, Aminoácidos Trypanosoma brucei brucei / genética Trypanosoma brucei brucei / metabolismo |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania RNA Trypanosoma brucei eIF4G |
| dc.subject.decs.pt_BR.fl_str_mv |
Sequência de Aminoácidos Animais Encadernação Sobrevivência Celular Fator de iniciação eucariótica-4G / metabolismo Leishmania major / genética Leishmania major / crescimento e desenvolvimento Leishmania major / metabolismo Dados de Sequencia Molecular Poly A / metabolismo Proteínas de Ligação Poli (A) / química Proteínas de Ligação Poli (A) / genética Proteínas de Ligação Poli (A) / metabolismo Ligação proteica Biossíntese de Proteínas Interferência de RNA RNA mensageiro / metabolismo Homologia de Sequência, Aminoácidos Trypanosoma brucei brucei / genética Trypanosoma brucei brucei / metabolismo |
| description |
Este trabalho foi parcialmente financiado por subsídios fornecidos pelas agências de fomento brasileiras FACEPE ( 006/2003-CNPq / PPP ) e CNPq ( 476255 / 2004-0 ). O TDDCL, o DMNM e o CRSR receberam bolsas de estudo da CAPES e FACEPE. O JRCV recebeu uma bolsa PQ (“pesquisador visitante”) da FIOCRUZ. O trabalho no laboratório de M. Carrington é financiado pelo Wellcome Trust, e LE recebeu uma bolsa de estudos financiada pelo BBSRC. |
| publishDate |
2010 |
| dc.date.issued.fl_str_mv |
2010 |
| dc.date.accessioned.fl_str_mv |
2018-04-27T12:06:31Z |
| dc.date.available.fl_str_mv |
2018-04-27T12:06:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
DA COSTA LIMA, T. D. et al. Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners. Eukaryotic Cell, v. 9, n. 10, p. 1484–1494, out. 2010. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/26166 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1535-9786 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/EC.00148-10 |
| identifier_str_mv |
DA COSTA LIMA, T. D. et al. Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners. Eukaryotic Cell, v. 9, n. 10, p. 1484–1494, out. 2010. 1535-9786 10.1128/EC.00148-10 |
| url |
https://www.arca.fiocruz.br/handle/icict/26166 |
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eng |
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eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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