Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19026 |
Resumo: | Oliveira, Ricardo Riccio “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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Pacheco, Flávia Thamiris FigueiredoSilva, Renata K N RMartins, Adson SantosOliveira, Ricardo RiccioNeves, Neuza Maria AlcântaraSilva, Moacir ParanhosSoares, Neci MatosTeixeira, Márcia Cristina Aquino2017-05-26T17:46:41Z2017-05-26T17:46:41Z2013PACHECO, F. T. F. et al. Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory. Journal of Parasitologists, v. 99, n. 6, p. 1002–1008, 2013.0022-3395https://www.arca.fiocruz.br/handle/icict/1902610.1645/12-33.1Oliveira, Ricardo Riccio “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/MCT), and Universidade Federal da Bahia (UFBA), Brazil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Laboratório Central de Saúde Pública Professor Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilDespite the availability of many parasitological methods for detection of Cryptosporidium and Isospora (Cystoisospora) belli in fecal samples, there are uncertainties about the accuracy of these techniques in laboratory practice. In this study, 27 formalin-fixed positive stool samples for Cryptosporidium and 15 for I. belli were analyzed by 2 concentration methods, sedimentation by centrifugation (SC) and formalin-ethyl acetate (FE), and by 3 tintorial techniques, modified Ziehl-Neelsen (ZN), safranin (SF), and auramine (AR). No significant differences were observed on Cryptosporidium identification between concentration methods, while a significantly higher number of I. belli oocysts (P < 0.0001) was detected in fecal smears concentrated by the SC than by the FE method. Fecal samples processed by FE produced a median oocyst loss to the fatty ring of 34.8% for Cryptosporidium and 45.4% for I. belli. However, FE concentration provided 63% of Cryptosporidium and 100% of I. belli slides classified as superior for microscopic examination. Regarding the efficiency of staining methods, a more significant detection of Cryptosporidium oocysts was observed in fecal smears stained by ZN (P < 0.01) or AR (P < 0.05) than by the SF method. Regular to high-quality slides for microscopic examination were mostly observed in fecal smears stained with AR or ZN for Cryptosporidium and with SF or ZN for I. belli. This study suggests a great variability in oocyst power detection by routine parasitological methods, and that the most frequent intestinal coccidians in humans have specific requirements for concentration and staining.engAmerican Society of ParasitologistsCryptosporidiumIsosporaDiarréiaColiformes fecaisLaboratório clinicoCryptosporidiumIsosporaDiarrheaFecal coliformsClinical laboratoryCryptosporidiumIsosporaDiarréiaDifferences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratoryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19026/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPacheco FT Differences in the 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| dc.title.pt_BR.fl_str_mv |
Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory |
| title |
Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory |
| spellingShingle |
Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory Pacheco, Flávia Thamiris Figueiredo Cryptosporidium Isospora Diarréia Coliformes fecais Laboratório clinico Cryptosporidium Isospora Diarrhea Fecal coliforms Clinical laboratory Cryptosporidium Isospora Diarréia |
| title_short |
Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory |
| title_full |
Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory |
| title_fullStr |
Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory |
| title_full_unstemmed |
Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory |
| title_sort |
Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory |
| author |
Pacheco, Flávia Thamiris Figueiredo |
| author_facet |
Pacheco, Flávia Thamiris Figueiredo Silva, Renata K N R Martins, Adson Santos Oliveira, Ricardo Riccio Neves, Neuza Maria Alcântara Silva, Moacir Paranhos Soares, Neci Matos Teixeira, Márcia Cristina Aquino |
| author_role |
author |
| author2 |
Silva, Renata K N R Martins, Adson Santos Oliveira, Ricardo Riccio Neves, Neuza Maria Alcântara Silva, Moacir Paranhos Soares, Neci Matos Teixeira, Márcia Cristina Aquino |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pacheco, Flávia Thamiris Figueiredo Silva, Renata K N R Martins, Adson Santos Oliveira, Ricardo Riccio Neves, Neuza Maria Alcântara Silva, Moacir Paranhos Soares, Neci Matos Teixeira, Márcia Cristina Aquino |
| dc.subject.other.pt_BR.fl_str_mv |
Cryptosporidium Isospora Diarréia Coliformes fecais Laboratório clinico |
| topic |
Cryptosporidium Isospora Diarréia Coliformes fecais Laboratório clinico Cryptosporidium Isospora Diarrhea Fecal coliforms Clinical laboratory Cryptosporidium Isospora Diarréia |
| dc.subject.en.pt_BR.fl_str_mv |
Cryptosporidium Isospora Diarrhea Fecal coliforms Clinical laboratory |
| dc.subject.decs.pt_BR.fl_str_mv |
Cryptosporidium Isospora Diarréia |
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Oliveira, Ricardo Riccio “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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2013 |
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2013 |
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2017-05-26T17:46:41Z |
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2017-05-26T17:46:41Z |
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PACHECO, F. T. F. et al. Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory. Journal of Parasitologists, v. 99, n. 6, p. 1002–1008, 2013. |
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https://www.arca.fiocruz.br/handle/icict/19026 |
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0022-3395 |
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10.1645/12-33.1 |
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PACHECO, F. T. F. et al. Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory. Journal of Parasitologists, v. 99, n. 6, p. 1002–1008, 2013. 0022-3395 10.1645/12-33.1 |
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American Society of Parasitologists |
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