Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35318 |
Resumo: | Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil. |
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Camaforte, Nathalia A. P. de PaulaSaldanha, Luiz LeonardoVareda, Priscilla Maria PonceRezende Neto, João M.Senger, Mario R.Delgado, Aislan Q.Morgan, Henrique J. N.Violato, Natalia MorettiPieroni, Laís GoyosDokkedal, Anne LígiaSilva Júnior, Floriano P.Bosqueiro, José Roberto2019-09-03T16:32:27Z2019-09-03T16:32:27Z2019CAMAFORTE, Nathalia A. P. de Paula et al. Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation. Pharmaceutical Biology, v. 57, n. 1, p. 269-279, 2019.1388-0209https://www.arca.fiocruz.br/handle/icict/3531810.1080/13880209.2019.15999621744-5116engTaylor & Francis OpenAntidiabéticoFlavonóides-O-glicosídeosFígadoLipídios plasmáticosFabaceaeAntidiabeticFabaceaeFlavonoid-O-glycosidesLiverPlasma lipidsHypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil.Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil.Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Drogas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Drogas. Rio de Janeiro, RJ, Brasil.Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil.Universidade do Estado de São Paulo. Departamento de Ciências Biológicas. Bauru, SP, Brasil.Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil.Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil.Universidade do Estado de São Paulo. Departamento de Ciências Biológicas. Bauru, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Drogas. Rio de Janeiro, RJ, Brasil.Universidade do Estado de São Paulo. Departamento de Educação Física. Bauru, SP, Brasil.Context: Bauhinia L. species, including Bauhinia holophylla (Bong.) Steud. (Fabaceae), have traditionally been used to treat diabetes. Bauhinia is a complex botanical genus, and the indiscriminate use of the diverse Bauhinia species is reflected in the experimental divergence of their medicinal potential. Objective: The hypoglycaemic and hypolipidaemic effects, molecular mechanism of action and phytochemical properties of an authentic extract of B. holophylla leaves were evaluated. Materials and methods: A phytochemical study of a 70% EtOH extract was performed using FIA-ESI-ITMS/MSn and HPLC-PAD-ESI-IT-MS. The extract (200 or 400 mg/kg b.w.) was administered for 14 days to streptozotocin-induced diabetic Swiss mice. Glucose tolerance and insulin sensitivity, blood parameters, gene and protein expression, and the in vivo and in vitro inhibition of intestinal glucosidases were assessed. Results: HPLC-PAD-ESI-IT-MS analysis identified flavonoid derivatives of quercetin, myricetin, luteolin and kaempferol. Treatment with 400 mg/kg of the extract reduced blood glucose (269.0 ± 32.4 mg/dL vs. 468.0 ± 32.2 mg/dL for diabetic animals), improved glucose tolerance, decreased cholesterol and triglyceride levels, and increased the mRNA expression of proteins involved in glucogenesis in the liver and muscle, such as PI3-K/Akt, GS, GSK3-b (ser-9), AMPK and Glut4. The activity of intestinal maltase was inhibited in vitro (IC50: 43.0 mg/mL for the extract compared to 516.4 mg/mL for acarbose) and in vivo. Discussion and conclusions: Treatment with B. holophylla was associated with a marked hypoglycaemic effect through the stimulation of glycogenesis and inhibition of gluconeogenesis and intestinal glucose absorption, without increasing basal insulinaemia.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35318/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarioSenger_JoaoRezendeNeto_etal_IOC_2019.pdfMarioSenger_JoaoRezendeNeto_etal_IOC_2019.pdfapplication/pdf1242476https://www.arca.fiocruz.br/bitstream/icict/35318/2/MarioSenger_JoaoRezendeNeto_etal_IOC_2019.pdfcf9cd058a9d337a23a23d6a7a7e02d4cMD52TEXTMarioSenger_JoaoRezendeNeto_etal_IOC_2019.pdf.txtMarioSenger_JoaoRezendeNeto_etal_IOC_2019.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation |
| title |
Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation |
| spellingShingle |
Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation Camaforte, Nathalia A. P. de Paula Antidiabético Flavonóides-O-glicosídeos Fígado Lipídios plasmáticos Fabaceae Antidiabetic Fabaceae Flavonoid-O-glycosides Liver Plasma lipids |
| title_short |
Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation |
| title_full |
Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation |
| title_fullStr |
Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation |
| title_full_unstemmed |
Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation |
| title_sort |
Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation |
| author |
Camaforte, Nathalia A. P. de Paula |
| author_facet |
Camaforte, Nathalia A. P. de Paula Saldanha, Luiz Leonardo Vareda, Priscilla Maria Ponce Rezende Neto, João M. Senger, Mario R. Delgado, Aislan Q. Morgan, Henrique J. N. Violato, Natalia Moretti Pieroni, Laís Goyos Dokkedal, Anne Lígia Silva Júnior, Floriano P. Bosqueiro, José Roberto |
| author_role |
author |
| author2 |
Saldanha, Luiz Leonardo Vareda, Priscilla Maria Ponce Rezende Neto, João M. Senger, Mario R. Delgado, Aislan Q. Morgan, Henrique J. N. Violato, Natalia Moretti Pieroni, Laís Goyos Dokkedal, Anne Lígia Silva Júnior, Floriano P. Bosqueiro, José Roberto |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Camaforte, Nathalia A. P. de Paula Saldanha, Luiz Leonardo Vareda, Priscilla Maria Ponce Rezende Neto, João M. Senger, Mario R. Delgado, Aislan Q. Morgan, Henrique J. N. Violato, Natalia Moretti Pieroni, Laís Goyos Dokkedal, Anne Lígia Silva Júnior, Floriano P. Bosqueiro, José Roberto |
| dc.subject.other.pt_BR.fl_str_mv |
Antidiabético Flavonóides-O-glicosídeos Fígado Lipídios plasmáticos Fabaceae |
| topic |
Antidiabético Flavonóides-O-glicosídeos Fígado Lipídios plasmáticos Fabaceae Antidiabetic Fabaceae Flavonoid-O-glycosides Liver Plasma lipids |
| dc.subject.en.pt_BR.fl_str_mv |
Antidiabetic Fabaceae Flavonoid-O-glycosides Liver Plasma lipids |
| description |
Universidade do Estado de São Paulo. Instituto de Biociências. Botucatu, SP, Brasil. |
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2019 |
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2019-09-03T16:32:27Z |
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2019-09-03T16:32:27Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CAMAFORTE, Nathalia A. P. de Paula et al. Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation. Pharmaceutical Biology, v. 57, n. 1, p. 269-279, 2019. |
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https://www.arca.fiocruz.br/handle/icict/35318 |
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1388-0209 |
| dc.identifier.doi.none.fl_str_mv |
10.1080/13880209.2019.1599962 |
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1744-5116 |
| identifier_str_mv |
CAMAFORTE, Nathalia A. P. de Paula et al. Hypoglycaemic activity of Bauhinia holophylla through GSK3-b inhibition and glycogenesis activation. Pharmaceutical Biology, v. 57, n. 1, p. 269-279, 2019. 1388-0209 10.1080/13880209.2019.1599962 1744-5116 |
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Taylor & Francis Open |
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Taylor & Francis Open |
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