Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions

Detalhes bibliográficos
Autor(a) principal: Sarno, Euzenir Nunes
Data de Publicação: 1987
Outros Autores: Sampaio, Elizabeth Pereira, Moreira, André Luís, Alvarenga, Fátima de Barros Fonseca
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33274
Resumo: Células inflamatórias presentes em lesões Virchowianas humanas foram isoladas do tecido por um processo de digestão enzimática. Elas foram mantidas em cultura por cerca de 5 dias e suas características morfológicas citoquímicas efuncionaisforam descritas.
id CRUZ_fe45e8d15ea0d5116452b503337171bd
oai_identifier_str oai:www.arca.fiocruz.br:icict/33274
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Sarno, Euzenir NunesSampaio, Elizabeth PereiraMoreira, André LuísAlvarenga, Fátima de Barros Fonseca2019-05-30T10:35:19Z2019-05-30T10:35:19Z1987SARNO, Euzenir Nunes et al. Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions. Revista da Sociedade Brasileira de Medicina Tropical, v. 20, n. 4, p. 205-207, out./dez. 1987.0037-8682https://www.arca.fiocruz.br/handle/icict/3327410.1590/S0037-868219870004000041678-9849Células inflamatórias presentes em lesões Virchowianas humanas foram isoladas do tecido por um processo de digestão enzimática. Elas foram mantidas em cultura por cerca de 5 dias e suas características morfológicas citoquímicas efuncionaisforam descritas.In the present study, inflammatory cells from human lepromatous lesions were isolated by enzymatic dissociation of tissue. They were maintained in culture up to five days and their morphologic, cythochemicaland functional characteristics were described.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Patologia Geral. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Patologia Geral. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Patologia Geral. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Medicas. Departamento de Patologia Geral. Rio de Janeiro, RJ, Brasil.engSociedade Brasileira de Medicina TropicalMacrófagoDissociação enzimáticaHanseníaseCélulas inflamatóriasMacrophageEnzymatic dissociationLeprosyInflammatory cellsIsolation and functional characterization of mononuclear phagocytes from human lepromatous lesionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/33274/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEuzenirSarno_ElizabethSampaio_etal_IOC_1987.pdfEuzenirSarno_ElizabethSampaio_etal_IOC_1987.pdfapplication/pdf633993https://www.arca.fiocruz.br/bitstream/icict/33274/2/EuzenirSarno_ElizabethSampaio_etal_IOC_1987.pdfc608bd36a15636a1f24face2bd0f8f85MD52TEXTEuzenirSarno_ElizabethSampaio_etal_IOC_1987.pdf.txtEuzenirSarno_ElizabethSampaio_etal_IOC_1987.pdf.txtExtracted texttext/plain12419https://www.arca.fiocruz.br/bitstream/icict/33274/3/EuzenirSarno_ElizabethSampaio_etal_IOC_1987.pdf.txtc7892a7eaf5b2f3a348e5b74466e3e8dMD53icict/332742023-09-05 10:30:42.582oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:30:42Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions
title Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions
spellingShingle Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions
Sarno, Euzenir Nunes
Macrófago
Dissociação enzimática
Hanseníase
Células inflamatórias
Macrophage
Enzymatic dissociation
Leprosy
Inflammatory cells
title_short Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions
title_full Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions
title_fullStr Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions
title_full_unstemmed Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions
title_sort Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions
author Sarno, Euzenir Nunes
author_facet Sarno, Euzenir Nunes
Sampaio, Elizabeth Pereira
Moreira, André Luís
Alvarenga, Fátima de Barros Fonseca
author_role author
author2 Sampaio, Elizabeth Pereira
Moreira, André Luís
Alvarenga, Fátima de Barros Fonseca
author2_role author
author
author
dc.contributor.author.fl_str_mv Sarno, Euzenir Nunes
Sampaio, Elizabeth Pereira
Moreira, André Luís
Alvarenga, Fátima de Barros Fonseca
dc.subject.other.pt_BR.fl_str_mv Macrófago
Dissociação enzimática
Hanseníase
Células inflamatórias
topic Macrófago
Dissociação enzimática
Hanseníase
Células inflamatórias
Macrophage
Enzymatic dissociation
Leprosy
Inflammatory cells
dc.subject.en.pt_BR.fl_str_mv Macrophage
Enzymatic dissociation
Leprosy
Inflammatory cells
description Células inflamatórias presentes em lesões Virchowianas humanas foram isoladas do tecido por um processo de digestão enzimática. Elas foram mantidas em cultura por cerca de 5 dias e suas características morfológicas citoquímicas efuncionaisforam descritas.
publishDate 1987
dc.date.issued.fl_str_mv 1987
dc.date.accessioned.fl_str_mv 2019-05-30T10:35:19Z
dc.date.available.fl_str_mv 2019-05-30T10:35:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SARNO, Euzenir Nunes et al. Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions. Revista da Sociedade Brasileira de Medicina Tropical, v. 20, n. 4, p. 205-207, out./dez. 1987.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33274
dc.identifier.issn.pt_BR.fl_str_mv 0037-8682
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S0037-86821987000400004
dc.identifier.eissn.none.fl_str_mv 1678-9849
identifier_str_mv SARNO, Euzenir Nunes et al. Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions. Revista da Sociedade Brasileira de Medicina Tropical, v. 20, n. 4, p. 205-207, out./dez. 1987.
0037-8682
10.1590/S0037-86821987000400004
1678-9849
url https://www.arca.fiocruz.br/handle/icict/33274
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33274/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33274/2/EuzenirSarno_ElizabethSampaio_etal_IOC_1987.pdf
https://www.arca.fiocruz.br/bitstream/icict/33274/3/EuzenirSarno_ElizabethSampaio_etal_IOC_1987.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
c608bd36a15636a1f24face2bd0f8f85
c7892a7eaf5b2f3a348e5b74466e3e8d
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813008907247812608