Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris

Detalhes bibliográficos
Autor(a) principal: Rocha, Isadora Filipaki Munhoz da
Data de Publicação: 2021
Outros Autores: Toledo, Martins, Sharon de, Amatuzzi, Rafaela Ferreira, Zamith-Miranda, Daniel, Nosanchuk, Joshua D., Rodrigues, Marcio Lourenço, Alves, Lysangela Ronalte
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50833
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
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spelling Rocha, Isadora Filipaki Munhoz daToledo, Martins, Sharon deAmatuzzi, Rafaela FerreiraZamith-Miranda, DanielNosanchuk, Joshua D.Rodrigues, Marcio LourençoAlves, Lysangela Ronalte2022-01-20T14:34:27Z2022-01-20T14:34:27Z2021ROCHA, Isadora Filipaki Munhoz da et al. Cellular and extracellular vesicle RNA analysis in the global threat fungus Candida auris. Veterinary World, p. 2735–2738, 2021.2165-0497https://www.arca.fiocruz.br/handle/icict/5083310.1128/Spectrum.01538-21porAmerican Society for MicrobiologyCellular and Extracellular vesicle RNA analysis in the global threat fungus Candida aurisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA. / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, New York, USA.Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA. / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, New York, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Emerging and reemerging pathogens are a worldwide concern, and it is predicted that these microbes will cause severe outbreaks. Candida auris affects people with weakened immune systems, particularly those who are hospitalized or are in health care facilities. Extracellular vesicles (EVs) are lipid bilayer structures released by organisms from all domains of life. EVs can deliver functional molecules to target cells, including proteins and nucleic acids, especially RNA molecules. EVs from several pathogenic fungi species play diverse biological roles related to cell-cell communication and pathogenhost interaction. In this study, we describe a data set which we produced by sequencing the RNA content of EVs from C. auris under normal growth conditions and in the presence of the antifungal caspofungin, a first-line drug to treat this fungus. To generate a more complete data set for future comparative studies, we also sequenced the RNA cellular content of EVs under the same conditions. This data set addresses a previously unexplored area of fungal biology regarding cellular small RNA and EV RNA. Our data will provide a molecular basis for the study of the aspects associated with antifungal treatment, gene expression response, and EV composition in C. auris. These data will also allow the exploration of small RNA content in the fungal kingdom and might serve as an informative basis for studies on the mechanisms by which molecules are directed to fungal EVs. Candida auris, a relevant emerging human-pathogenic yeast, is the first fungus to be called a global public health threat by the WHO. This is because of its rapid spread on all inhabited continents, together with its extremely high frequency of drug and multidrug resistance. In our study, we generated a large data set for 3 distinct strains of C. auris and obtained cellular small RNA fraction as well as extracellular vesicle RNA (EV-RNA) during normal growth conditions and after treatment with caspofungin, the first-line drug used to treat C. auris infection.Extracellular VesiclesVesículas ExtracelularesMicroARNsVésicules extracellulairesmicroARNCandida aurisRNA-SeqVesículas ExtracelularesMicroRNAsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50833/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALSpectrum.01538-21Ok.pdfSpectrum.01538-21Ok.pdfapplication/pdf2402434https://www.arca.fiocruz.br/bitstream/icict/50833/2/Spectrum.01538-21Ok.pdff2c0319ecd41c00b3476854cc2ff672cMD52icict/508332022-01-20 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dc.title.pt_BR.fl_str_mv Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris
title Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris
spellingShingle Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris
Rocha, Isadora Filipaki Munhoz da
Extracellular Vesicles
Vesículas Extracelulares
MicroARNs
Vésicules extracellulaires
microARN
Candida auris
RNA-Seq
Vesículas Extracelulares
MicroRNAs
title_short Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris
title_full Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris
title_fullStr Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris
title_full_unstemmed Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris
title_sort Cellular and Extracellular vesicle RNA analysis in the global threat fungus Candida auris
author Rocha, Isadora Filipaki Munhoz da
author_facet Rocha, Isadora Filipaki Munhoz da
Toledo, Martins, Sharon de
Amatuzzi, Rafaela Ferreira
Zamith-Miranda, Daniel
Nosanchuk, Joshua D.
Rodrigues, Marcio Lourenço
Alves, Lysangela Ronalte
author_role author
author2 Toledo, Martins, Sharon de
Amatuzzi, Rafaela Ferreira
Zamith-Miranda, Daniel
Nosanchuk, Joshua D.
Rodrigues, Marcio Lourenço
Alves, Lysangela Ronalte
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rocha, Isadora Filipaki Munhoz da
Toledo, Martins, Sharon de
Amatuzzi, Rafaela Ferreira
Zamith-Miranda, Daniel
Nosanchuk, Joshua D.
Rodrigues, Marcio Lourenço
Alves, Lysangela Ronalte
dc.subject.en.pt_BR.fl_str_mv Extracellular Vesicles
topic Extracellular Vesicles
Vesículas Extracelulares
MicroARNs
Vésicules extracellulaires
microARN
Candida auris
RNA-Seq
Vesículas Extracelulares
MicroRNAs
dc.subject.es.pt_BR.fl_str_mv Vesículas Extracelulares
MicroARNs
dc.subject.fr.pt_BR.fl_str_mv Vésicules extracellulaires
microARN
dc.subject.decs.pt_BR.fl_str_mv Candida auris
RNA-Seq
Vesículas Extracelulares
MicroRNAs
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-20T14:34:27Z
dc.date.available.fl_str_mv 2022-01-20T14:34:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROCHA, Isadora Filipaki Munhoz da et al. Cellular and extracellular vesicle RNA analysis in the global threat fungus Candida auris. Veterinary World, p. 2735–2738, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50833
dc.identifier.issn.pt_BR.fl_str_mv 2165-0497
dc.identifier.doi.none.fl_str_mv 10.1128/Spectrum.01538-21
identifier_str_mv ROCHA, Isadora Filipaki Munhoz da et al. Cellular and extracellular vesicle RNA analysis in the global threat fungus Candida auris. Veterinary World, p. 2735–2738, 2021.
2165-0497
10.1128/Spectrum.01538-21
url https://www.arca.fiocruz.br/handle/icict/50833
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50833/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50833/2/Spectrum.01538-21Ok.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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