Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery

Detalhes bibliográficos
Autor(a) principal: Abreu, Ednéa Oliveira de
Data de Publicação: 2018
Outros Autores: Santos, Danielle Silva dos, Lepletier, Ailin, Ramos, Tiago D. P., Reis, Rafaella Ferreira, Fontes, Larissa Vasconcelos, Ramos, Mariana T., Torres, Rafael C., Almeida, Vinícius Cotta de, Carvalho, Vinícius de Frias, Villa-Verde, Déa M. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29457
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
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spelling Abreu, Ednéa Oliveira deSantos, Danielle Silva dosLepletier, AilinRamos, Tiago D. P.Reis, Rafaella FerreiraFontes, Larissa VasconcelosRamos, Mariana T.Torres, Rafael C.Almeida, Vinícius Cotta deCarvalho, Vinícius de FriasVilla-Verde, Déa M. S.2018-10-09T13:26:40Z2018-10-09T13:26:40Z2018ABREU, Ednéa Oliveira de Abreu; et al. Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery. Frontiers in Endocrinology, v.9, Article 365, 13p, July 2018.1664-2392https://www.arca.fiocruz.br/handle/icict/2945710.3389/fendo.2018.00365engFrontiers MediaTimotimócitosproliferaçãoGlucocorticoidesMorte celularGalectina 3galectin-3thymusthymocytesproliferationcell deathglucocorticoidsteroidogenic machineryLack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machineryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Maintenance of thymus homeostasis is a delicate interplay involving hormones, neurotransmitters and local microenvironmental proteins, as well as saccharides, acting on both thymocytes and stromal cells. Disturbances in these interactions may lead to alterations on thymocyte development. We previously showed that galectin-3, a β-galactoside-binding protein, is constitutively expressed in the thymus, interacting with extracellular matrix glycoproteins and acting as a de-adhesion molecule, thus modulating thymocyte-stromal cell interactions. In this work, we aimed to investigate the participation of galectin-3 in the maintenance of thymus homeostasis, including hormonal-mediated circuits. For that, we used genetically engineered galectin-3-deficient mice. We observed that the thymus of galectin-3-deficient mice was reduced in mass and cellularity compared to wild-type controls; however, the proportions of different thymocyte subpopulations defined by CD4/CD8 expression were not changed. Considering the CD4-CD8- double-negative (DN) subpopulation, an accumulation of the most immature (DN1) stage was observed. Additionally, the proliferative capacity of thymocytes was decreased in all thymocyte subsets, whereas the percentage of apoptosis was increased, especially in the CD4+CD8+ double-positive thymocytes. As glucocorticoid hormones are known to be involved in thymus homeostasis, we evaluated serum and intrathymic corticosterone levels by radioimmunoassay, and the expression of steroidogenic machinery using real-time PCR. We detected a significant increase in corticosterone levels in both serum and thymus samples of galectin-3-deficient mice, as compared to age-matched controls. This was paralleled by an increase of gene transcription of the steroidogenic enzymes, steroidogenic acute regulatory protein (Star) and Cyp11b1 in thymus, 11β-Hydroxysteroid Dehydrogenase (Hsd11b1) in the adrenal, and Cyp11a1 in both glands. In conclusion, our findings show that the absence of galectin-3 subverts mouse thymus homeostasis by a mechanism likely associated to intrathymic and systemic stress-related endocrine circuitries, affecting thymocyte number, proliferation and apoptosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29457/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALedneaO_abreu_etal_IOC_2018.pdfedneaO_abreu_etal_IOC_2018.pdfapplication/pdf4005349https://www.arca.fiocruz.br/bitstream/icict/29457/2/edneaO_abreu_etal_IOC_2018.pdf6bab5839acd772e48032b95e4145e894MD52TEXTedneaO_abreu_etal_IOC_2018.pdf.txtedneaO_abreu_etal_IOC_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery
title Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery
spellingShingle Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery
Abreu, Ednéa Oliveira de
Timo
timócitos
proliferação
Glucocorticoides
Morte celular
Galectina 3
galectin-3
thymus
thymocytes
proliferation
cell death
glucocorticoid
steroidogenic machinery
title_short Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery
title_full Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery
title_fullStr Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery
title_full_unstemmed Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery
title_sort Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery
author Abreu, Ednéa Oliveira de
author_facet Abreu, Ednéa Oliveira de
Santos, Danielle Silva dos
Lepletier, Ailin
Ramos, Tiago D. P.
Reis, Rafaella Ferreira
Fontes, Larissa Vasconcelos
Ramos, Mariana T.
Torres, Rafael C.
Almeida, Vinícius Cotta de
Carvalho, Vinícius de Frias
Villa-Verde, Déa M. S.
author_role author
author2 Santos, Danielle Silva dos
Lepletier, Ailin
Ramos, Tiago D. P.
Reis, Rafaella Ferreira
Fontes, Larissa Vasconcelos
Ramos, Mariana T.
Torres, Rafael C.
Almeida, Vinícius Cotta de
Carvalho, Vinícius de Frias
Villa-Verde, Déa M. S.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Abreu, Ednéa Oliveira de
Santos, Danielle Silva dos
Lepletier, Ailin
Ramos, Tiago D. P.
Reis, Rafaella Ferreira
Fontes, Larissa Vasconcelos
Ramos, Mariana T.
Torres, Rafael C.
Almeida, Vinícius Cotta de
Carvalho, Vinícius de Frias
Villa-Verde, Déa M. S.
dc.subject.other.pt_BR.fl_str_mv Timo
timócitos
proliferação
Glucocorticoides
Morte celular
Galectina 3
topic Timo
timócitos
proliferação
Glucocorticoides
Morte celular
Galectina 3
galectin-3
thymus
thymocytes
proliferation
cell death
glucocorticoid
steroidogenic machinery
dc.subject.en.pt_BR.fl_str_mv galectin-3
thymus
thymocytes
proliferation
cell death
glucocorticoid
steroidogenic machinery
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-10-09T13:26:40Z
dc.date.available.fl_str_mv 2018-10-09T13:26:40Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ABREU, Ednéa Oliveira de Abreu; et al. Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery. Frontiers in Endocrinology, v.9, Article 365, 13p, July 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29457
dc.identifier.issn.pt_BR.fl_str_mv 1664-2392
dc.identifier.doi.none.fl_str_mv 10.3389/fendo.2018.00365
identifier_str_mv ABREU, Ednéa Oliveira de Abreu; et al. Lack of Galectin-3 Disrupts Thymus Homeostasis in Association to Increase of Local and Systemic Glucocorticoid Levels and Steroidogenic Machinery. Frontiers in Endocrinology, v.9, Article 365, 13p, July 2018.
1664-2392
10.3389/fendo.2018.00365
url https://www.arca.fiocruz.br/handle/icict/29457
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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