Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11868 |
Resumo: | Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde (INCQS). Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Ministério da Defesa. Laboratório Químico Farmacêutico do Exército. Rio de Janeiro, RJ, Brasil. |
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Baio, Paulo Victor PereiraRamos, Juliana NunesSantos, Louisy Sanches dosSoriano, Morgana FonsecaLadeira, Elisa MartinsSouza, Mônica CristinaCamello, Thereza Cristina FerreiraRibeiro, Marcio GarciaHirata Junior, RaphaelVieira, Verônica VianaGuaraldi, Ana Luíza Mattos2015-10-02T12:45:05Z2015-10-02T12:45:05Z2013BAIO, Paulo Victor Pereira; et al. Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil. Plos One, v.7, n.12, e2573, 10p, Dec. 2013.https://www.arca.fiocruz.br/handle/icict/1186810.1371/journal.pntd.0002573engPlos OneBrasilNocardiaHuman NocardiosisBrazilNocardiaMolecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde (INCQS). Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Ministério da Defesa. Laboratório Químico Farmacêutico do Exército. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde (INCQS). Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde (INCQS). Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro – UERJ, Hospital Universita´rio Pedro Ernesto, Laborato´ rio de Bacteriologia, Rio de Janeiro, Rio de Janeiro, Brasil.Universidade Estadual Paulista – UNESP. Faculdade de Medicina Veterinária e Zootecnia. Botucatu, SP, Brasil.Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde (INCQS). Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil.Background: Nocardia sp. causes a variety of clinical presentations. The incidence of nocardiosis varies geographically according to several factors, such as the prevalence of HIV infections, transplants, neoplastic and rheumatic diseases, as well as climate, socio-economic conditions and laboratory procedures for Nocardia detection and identification. In Brazil the paucity of clinical reports of Nocardia infections suggests that this genus may be underestimated as a cause of human diseases and/or either neglected or misidentified in laboratory specimens. Accurate identification of Nocardia species has become increasingly important for clinical and epidemiological investigations. In this study, seven clinical Nocardia isolates were identified by multilocus sequence analysis (MLSA) and their antimicrobial susceptibility was also determined. Most Nocardia isolates were associated to pulmonary disease. Methodology/Principal Findings: The majority of Brazilian human isolates in cases reported in literature were identified as Nocardia sp. Molecular characterization was used for species identification of Nocardia nova, Nocardia cyriacigeorgica, Nocardia asiatica and Nocardia exalbida/gamkensis. Data indicated that molecular analysis provided a different Nocardia speciation than the initial biochemical identification for most Brazilian isolates. All Nocardia isolates showed susceptibility to trimethoprim-sulfamethoxazole, the antimicrobial of choice in the treatment nocardiosis. N. nova isolated from different clinical specimens from one patient showed identical antimicrobial susceptibility patterns and two distinct clones. Conclusions/Significance: Although Brazil is the world’s fifth-largest country in terms of land mass and population, pulmonary, extrapulmonary and systemic forms of nocardiosis were reported in only 6 of the 26 Brazilian states from 1970 to 2013. A least 33.8% of these 46 cases of nocardiosis proved fatal. Interestingly, coinfection by two clones may occur in patients presenting nocardiosis. Nocardia infection may be more common throughout the Brazilian territory and in other developing tropical countries than is currently recognized and MLSA should be used more extensively as an effective method for Nocardia identification.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11868/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALpaulo_baio_etal_IOC_2013.pdfapplication/pdf403771https://www.arca.fiocruz.br/bitstream/icict/11868/2/paulo_baio_etal_IOC_2013.pdf90674e7a633ac4d53cbe32e991010a4eMD52TEXTpaulo_baio_etal_IOC_2013.pdf.txtpaulo_baio_etal_IOC_2013.pdf.txtExtracted texttext/plain61856https://www.arca.fiocruz.br/bitstream/icict/11868/3/paulo_baio_etal_IOC_2013.pdf.txt63e3db2229895f996ab2bb1317fcf9b6MD53icict/118682022-06-24 12:17:36.842oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil |
| title |
Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil |
| spellingShingle |
Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil Baio, Paulo Victor Pereira Brasil Nocardia Human Nocardiosis Brazil Nocardia |
| title_short |
Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil |
| title_full |
Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil |
| title_fullStr |
Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil |
| title_full_unstemmed |
Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil |
| title_sort |
Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil |
| author |
Baio, Paulo Victor Pereira |
| author_facet |
Baio, Paulo Victor Pereira Ramos, Juliana Nunes Santos, Louisy Sanches dos Soriano, Morgana Fonseca Ladeira, Elisa Martins Souza, Mônica Cristina Camello, Thereza Cristina Ferreira Ribeiro, Marcio Garcia Hirata Junior, Raphael Vieira, Verônica Viana Guaraldi, Ana Luíza Mattos |
| author_role |
author |
| author2 |
Ramos, Juliana Nunes Santos, Louisy Sanches dos Soriano, Morgana Fonseca Ladeira, Elisa Martins Souza, Mônica Cristina Camello, Thereza Cristina Ferreira Ribeiro, Marcio Garcia Hirata Junior, Raphael Vieira, Verônica Viana Guaraldi, Ana Luíza Mattos |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Baio, Paulo Victor Pereira Ramos, Juliana Nunes Santos, Louisy Sanches dos Soriano, Morgana Fonseca Ladeira, Elisa Martins Souza, Mônica Cristina Camello, Thereza Cristina Ferreira Ribeiro, Marcio Garcia Hirata Junior, Raphael Vieira, Verônica Viana Guaraldi, Ana Luíza Mattos |
| dc.subject.other.pt_BR.fl_str_mv |
Brasil |
| topic |
Brasil Nocardia Human Nocardiosis Brazil Nocardia |
| dc.subject.en.pt_BR.fl_str_mv |
Nocardia Human Nocardiosis Brazil |
| dc.subject.decs.pt_BR.fl_str_mv |
Nocardia |
| description |
Universidade do Estado do Rio de Janeiro – UERJ. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Patologia. Laboratório de Difteria e Corinebactérias de Importância Clínica-LDCIC. Centro Colaborador para Difteria da CGLAB/SVS/MS. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde (INCQS). Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Ministério da Defesa. Laboratório Químico Farmacêutico do Exército. Rio de Janeiro, RJ, Brasil. |
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2013 |
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2013 |
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2015-10-02T12:45:05Z |
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2015-10-02T12:45:05Z |
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BAIO, Paulo Victor Pereira; et al. Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil. Plos One, v.7, n.12, e2573, 10p, Dec. 2013. |
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https://www.arca.fiocruz.br/handle/icict/11868 |
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10.1371/journal.pntd.0002573 |
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BAIO, Paulo Victor Pereira; et al. Molecular Identification of Nocardia Isolates from Clinical Samples and an Overview of Human Nocardiosis in Brazil. Plos One, v.7, n.12, e2573, 10p, Dec. 2013. 10.1371/journal.pntd.0002573 |
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