Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia

Detalhes bibliográficos
Autor(a) principal: Ramos, Hugo Macedo
Data de Publicação: 2016
Outros Autores: Ruiz-Mendoza, Susana, Mariante, Rafael M., Guimarães, Erick V., Souza, Lucas C. Quadros de, Paiva, Mauricio M., Ferreira, Eliane de O, Pinto, Tatiana C. A., Teixeira, Lucia M., Allodi, Silvana, Cruz, Wagner Baetas da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18218
Resumo: Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Centro de Cirurgia Experimental. Laboratório Translacional em Fisiologia Molecular.Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Program de Pós-Graduação em Ciências Biológicas (Fisiologia). Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho..Laboratório de Neurobiologia Comparativa e do Desenvolvimento. Rio de Janeiro, RJ., Brasil.
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spelling Ramos, Hugo MacedoRuiz-Mendoza, SusanaMariante, Rafael M.Guimarães, Erick V.Souza, Lucas C. Quadros dePaiva, Mauricio M.Ferreira, Eliane de OPinto, Tatiana C. A.Teixeira, Lucia M.Allodi, SilvanaCruz, Wagner Baetas da2017-03-30T15:55:42Z2017-03-30T15:55:42Z2016RAMOS, Hugo Macedo; et al. Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia. Scientific Reports, v.6:36813, 11p, Nov. 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/1821810.1038/srep36813engNature Publishing GroupStreptococcus pneumoniaemicrogliaEspaço IntracelularCélulas olfatóriasStreptococcus pneumoniaemicrogliaolfactory ensheathing cellsintracellular killingStreptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microgliainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Faculdade de Medicina. Centro de Cirurgia Experimental. Laboratório Translacional em Fisiologia Molecular.Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Program de Pós-Graduação em Ciências Biológicas (Fisiologia). Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho..Laboratório de Neurobiologia Comparativa e do Desenvolvimento. Rio de Janeiro, RJ., Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Centro de Cirurgia Experimental. Laboratório Translacional em Fisiologia Molecular.Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Programa de Pós-Graduação em Ciências Biológicas (Fisiologia). Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurogênese. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Centro de Cirurgia Experimental. Laboratório Translacional em Fisiologia Molecular.Rio de Janeiro, RJ, Brasil.Instituto Nacional de Tecnologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro - Polo Xerém. Duque de Caxias, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Programa de Pós-Graduação em Ciências Biológicas (Fisiologia). Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho..Laboratório de Neurobiologia Comparativa e do Desenvolvimento. Rio de Janeiro, RJ., Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Centro de Cirurgia Experimental. Laboratório Translacional em Fisiologia Molecular.Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Programa de Pós-Graduação em Ciências Biológicas (Fisiologia). Rio de Janeiro, RJ, Brasil.Olfactory ensheathing cells (OECs) are a type of specialized glial cell currently considered as having a double function in the nervous system: one regenerative, and another immune. Streptococcus pneumoniae is a major agent of severe infections in humans, including meningitis. It is commonly found in the nasopharynx of asymptomatic carriers, and, under certain still unknown conditions, can invade the brain. We evaluated whether pneumococcal cells recovered from lysed OECs and microglia are able to survive by manipulating the host cell activation. An intracellular-survival assay of S. pneumoniae in OECs showed a significant number of bacterial CFU recovered after 3 h of infection. In contrast, microglia assays resulted in a reduced number of CFU. Electron-microscopy analysis revealed a large number of pneumococci with apparently intact morphology. However, microglia cells showed endocytic vesicles containing only bacterial cell debris. Infection of OEC cultures resulted in continuous NF-κB activation. The IFN-γ-induced increase of iNOS expression was reversed in infected OECs. OECs are susceptible to S. pneumoniae infection, which can suppress their cytotoxic mechanisms in order to survive. We suggest that, in contrast to microglia, OECs might serve as safe targets for pneumococci, providing a more stable environment for evasion of the immune system.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18218/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALerick_guimaraes_etal_IOC_2016.pdferick_guimaraes_etal_IOC_2016.pdfapplication/pdf1840608https://www.arca.fiocruz.br/bitstream/icict/18218/2/erick_guimaraes_etal_IOC_2016.pdf68d196aacadd428af5c3a982825f8a4dMD52TEXTerick_guimaraes_etal_IOC_2016.pdf.txterick_guimaraes_etal_IOC_2016.pdf.txtExtracted texttext/plain45034https://www.arca.fiocruz.br/bitstream/icict/18218/3/erick_guimaraes_etal_IOC_2016.pdf.txt3c65308326e47beca7b1c46f8c4b5368MD53icict/182182018-08-15 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dc.title.pt_BR.fl_str_mv Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia
title Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia
spellingShingle Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia
Ramos, Hugo Macedo
Streptococcus pneumoniae
microglia
Espaço Intracelular
Células olfatórias
Streptococcus pneumoniae
microglia
olfactory ensheathing cells
intracellular killing
title_short Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia
title_full Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia
title_fullStr Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia
title_full_unstemmed Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia
title_sort Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia
author Ramos, Hugo Macedo
author_facet Ramos, Hugo Macedo
Ruiz-Mendoza, Susana
Mariante, Rafael M.
Guimarães, Erick V.
Souza, Lucas C. Quadros de
Paiva, Mauricio M.
Ferreira, Eliane de O
Pinto, Tatiana C. A.
Teixeira, Lucia M.
Allodi, Silvana
Cruz, Wagner Baetas da
author_role author
author2 Ruiz-Mendoza, Susana
Mariante, Rafael M.
Guimarães, Erick V.
Souza, Lucas C. Quadros de
Paiva, Mauricio M.
Ferreira, Eliane de O
Pinto, Tatiana C. A.
Teixeira, Lucia M.
Allodi, Silvana
Cruz, Wagner Baetas da
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ramos, Hugo Macedo
Ruiz-Mendoza, Susana
Mariante, Rafael M.
Guimarães, Erick V.
Souza, Lucas C. Quadros de
Paiva, Mauricio M.
Ferreira, Eliane de O
Pinto, Tatiana C. A.
Teixeira, Lucia M.
Allodi, Silvana
Cruz, Wagner Baetas da
dc.subject.other.pt_BR.fl_str_mv Streptococcus pneumoniae
microglia
Espaço Intracelular
Células olfatórias
topic Streptococcus pneumoniae
microglia
Espaço Intracelular
Células olfatórias
Streptococcus pneumoniae
microglia
olfactory ensheathing cells
intracellular killing
dc.subject.en.pt_BR.fl_str_mv Streptococcus pneumoniae
microglia
olfactory ensheathing cells
intracellular killing
description Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Centro de Cirurgia Experimental. Laboratório Translacional em Fisiologia Molecular.Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Program de Pós-Graduação em Ciências Biológicas (Fisiologia). Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho..Laboratório de Neurobiologia Comparativa e do Desenvolvimento. Rio de Janeiro, RJ., Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-30T15:55:42Z
dc.date.available.fl_str_mv 2017-03-30T15:55:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMOS, Hugo Macedo; et al. Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia. Scientific Reports, v.6:36813, 11p, Nov. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18218
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/srep36813
identifier_str_mv RAMOS, Hugo Macedo; et al. Streptococcus pneumoniae resists intracellular killing by olfactory ensheathing cells but not by microglia. Scientific Reports, v.6:36813, 11p, Nov. 2016.
2045-2322
10.1038/srep36813
url https://www.arca.fiocruz.br/handle/icict/18218
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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