Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection

Detalhes bibliográficos
Autor(a) principal: Camandaroba, Edson Luiz Paes
Data de Publicação: 2006
Outros Autores: Reis, Eliana Almeida Gomes, Reis, Mitermayer Galvão dos, Andrade, Sonia Gumes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6197
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
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spelling Camandaroba, Edson Luiz PaesReis, Eliana Almeida GomesReis, Mitermayer Galvão dosAndrade, Sonia Gumes2013-01-15T17:45:01Z2013-01-15T17:45:01Z2006CAMANDAROBA, E. L. P. et al. Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection. Memórias do Instituto Oswaldo Cruz, v. 101, n. 6, p. 613-615, sept. 2006.0074-0276https://www.arca.fiocruz.br/handle/icict/6197engSchizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilColombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment lenght polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.Trypanosoma cruziColombian strain clonesSchizodemeDNA de Cinetoplasto/genéticaTrypanosoma cruzi/genéticaDoença AgudaDoença de Chagas/parasitologiaCélulas ClonaisColômbiaReação em Cadeia da PolimerasePolimorfismo de Fragmento de RestriçãoTrypanosoma cruzi/enzimologiaTrypanosoma cruzi/patogenicidadeVirulênciaAnimaisCamundongosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCamandaroba, Edson L. P. et al. Schizodeme analysis.....pdfCamandaroba, Edson L. P. et al. 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dc.title.pt_BR.fl_str_mv Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
spellingShingle Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
Camandaroba, Edson Luiz Paes
Trypanosoma cruzi
Colombian strain clones
Schizodeme
DNA de Cinetoplasto/genética
Trypanosoma cruzi/genética
Doença Aguda
Doença de Chagas/parasitologia
Células Clonais
Colômbia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Trypanosoma cruzi/enzimologia
Trypanosoma cruzi/patogenicidade
Virulência
Animais
Camundongos
title_short Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title_full Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title_fullStr Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title_full_unstemmed Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title_sort Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
author Camandaroba, Edson Luiz Paes
author_facet Camandaroba, Edson Luiz Paes
Reis, Eliana Almeida Gomes
Reis, Mitermayer Galvão dos
Andrade, Sonia Gumes
author_role author
author2 Reis, Eliana Almeida Gomes
Reis, Mitermayer Galvão dos
Andrade, Sonia Gumes
author2_role author
author
author
dc.contributor.author.fl_str_mv Camandaroba, Edson Luiz Paes
Reis, Eliana Almeida Gomes
Reis, Mitermayer Galvão dos
Andrade, Sonia Gumes
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Colombian strain clones
Schizodeme
topic Trypanosoma cruzi
Colombian strain clones
Schizodeme
DNA de Cinetoplasto/genética
Trypanosoma cruzi/genética
Doença Aguda
Doença de Chagas/parasitologia
Células Clonais
Colômbia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Trypanosoma cruzi/enzimologia
Trypanosoma cruzi/patogenicidade
Virulência
Animais
Camundongos
dc.subject.decs.pt_BR.fl_str_mv DNA de Cinetoplasto/genética
Trypanosoma cruzi/genética
Doença Aguda
Doença de Chagas/parasitologia
Células Clonais
Colômbia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Trypanosoma cruzi/enzimologia
Trypanosoma cruzi/patogenicidade
Virulência
Animais
Camundongos
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2013-01-15T17:45:01Z
dc.date.available.fl_str_mv 2013-01-15T17:45:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CAMANDAROBA, E. L. P. et al. Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection. Memórias do Instituto Oswaldo Cruz, v. 101, n. 6, p. 613-615, sept. 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6197
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv CAMANDAROBA, E. L. P. et al. Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection. Memórias do Instituto Oswaldo Cruz, v. 101, n. 6, p. 613-615, sept. 2006.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/6197
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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