Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line

Detalhes bibliográficos
Autor(a) principal: Echevarria-Lima, J.
Data de Publicação: 2005
Outros Autores: Kyle-Cezar, F., Leite, D. F. P., Capella, L., Capella, M. A., Rumjanek, V. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33611
Resumo: Juliana Echevarria-Lima. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
id CRUZ_ff9bdc3ce2a0bc99f40c35fe1580a1bb
oai_identifier_str oai:www.arca.fiocruz.br:icict/33611
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Echevarria-Lima, J.Kyle-Cezar, F.Leite, D. F. P.Capella, L.Capella, M. A.Rumjanek, V. M.2019-06-20T22:50:03Z2019-06-20T22:50:03Z2005ECHEVARIA-LIMA, Juliana et al. Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line. Immunology, v. 114, n. 4, p. 468-475, 2005.0019-2805https://www.arca.fiocruz.br/handle/icict/3361110.1111/j.1365-2567.2005.02116.x1365-2567Juliana Echevarria-Lima. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.2020-06-20Universidade Federal do Rio de Janeiro. Departamento de Bioquímica Médica. Laboratório de Imunologia Tumoral. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Bioquímica Médica. Laboratório de Imunologia Tumoral. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade do Rio de Janeiro. Departamento de Clínica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Bioquímica Médica. Laboratório de Imunologia Tumoral. Rio de Janeiro, RJ, Brasil.Multidrug resistance proteins [MRPs and P-glycoprotein (Pgp)] are members of the family of ATP-binding cassette (ABC) transport proteins, originally described as being involved in the resistance against anti-cancer agents in tumour cells. These proteins act as ATP-dependent efflux pumps and have now been described in normal cells where they exert physiological roles. The aim of this work was to investigate the expression and activity of MRP and Pgp in the thymoma cell line, EL4. It was observed that EL4 cells expressed mRNA for MRP1, but not for MRP2, MRP3 or Pgp. The activity of ABC transport proteins was evaluated by using the efflux of the fluorescent probes carboxy-20-70 -dichlorofluorescein diacetate (CFDA) and rhodamine 123 (Rho 123). EL4 cells did not retain CFDAintracellularly, and MRP inhibitors (probenecid, indomethacin and MK 571) decreased MRP1 activity in a concentration-dependent manner. As expected, EL4 cells accumulated Rho 123, and the presence of cyclosporin A and verapamil did not modify this accumulation. Most importantly, when EL4 cells were incubated in the presence of the MRP1 inhibitors indomethacin and MK 571 for 6 days, they started to express CD4 and CD8 molecules on their surface, producing double-positive cells and CD8 single-positive cells. Our results suggest that MRP activity is important for the maintenance of the undifferentiated state in this cell type. This finding might have implications in the physiological process of normal thymocyte maturation.engWileyExpression and activity of multidrug resistance protein 1 in a murine thymoma cell lineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleIndomethacinMK 571MRP inhibitorsMRPThymoma cell lineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33611/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Echevarria-Lima_Juliana_etal_INI_2005.pdfve_Echevarria-Lima_Juliana_etal_INI_2005.pdfapplication/pdf379474https://www.arca.fiocruz.br/bitstream/icict/33611/2/ve_Echevarria-Lima_Juliana_etal_INI_2005.pdf5a57895a1eede1e3c5f0fa3c3758c449MD52TEXTve_Echevarria-Lima_Juliana_etal_INI_2005.pdf.txtve_Echevarria-Lima_Juliana_etal_INI_2005.pdf.txtExtracted texttext/plain34589https://www.arca.fiocruz.br/bitstream/icict/33611/3/ve_Echevarria-Lima_Juliana_etal_INI_2005.pdf.txt8fa59e691b8ff80c18438dc2c6c577c4MD53icict/336112021-03-24 16:32:33.237oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
title Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
spellingShingle Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
Echevarria-Lima, J.
Indomethacin
MK 571
MRP inhibitors
MRP
Thymoma cell line
title_short Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
title_full Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
title_fullStr Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
title_full_unstemmed Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
title_sort Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
author Echevarria-Lima, J.
author_facet Echevarria-Lima, J.
Kyle-Cezar, F.
Leite, D. F. P.
Capella, L.
Capella, M. A.
Rumjanek, V. M.
author_role author
author2 Kyle-Cezar, F.
Leite, D. F. P.
Capella, L.
Capella, M. A.
Rumjanek, V. M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Echevarria-Lima, J.
Kyle-Cezar, F.
Leite, D. F. P.
Capella, L.
Capella, M. A.
Rumjanek, V. M.
dc.subject.en.pt_BR.fl_str_mv Indomethacin
MK 571
MRP inhibitors
MRP
Thymoma cell line
topic Indomethacin
MK 571
MRP inhibitors
MRP
Thymoma cell line
description Juliana Echevarria-Lima. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2019-06-20T22:50:03Z
dc.date.available.fl_str_mv 2019-06-20T22:50:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ECHEVARIA-LIMA, Juliana et al. Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line. Immunology, v. 114, n. 4, p. 468-475, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33611
dc.identifier.issn.pt_BR.fl_str_mv 0019-2805
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2567.2005.02116.x
dc.identifier.eissn.none.fl_str_mv 1365-2567
identifier_str_mv ECHEVARIA-LIMA, Juliana et al. Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line. Immunology, v. 114, n. 4, p. 468-475, 2005.
0019-2805
10.1111/j.1365-2567.2005.02116.x
1365-2567
url https://www.arca.fiocruz.br/handle/icict/33611
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33611/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33611/2/ve_Echevarria-Lima_Juliana_etal_INI_2005.pdf
https://www.arca.fiocruz.br/bitstream/icict/33611/3/ve_Echevarria-Lima_Juliana_etal_INI_2005.pdf.txt
bitstream.checksum.fl_str_mv 8a4605be74aa9ea9d79846c1fba20a33
5a57895a1eede1e3c5f0fa3c3758c449
8fa59e691b8ff80c18438dc2c6c577c4
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009092101275648

Registros relacionados