A aplicabilidade da colpocitologia oncótica para o rastreamento das alterações celulares causadas pelo papiloma vírus humano

• Autor(a) principal: Souza, Deise da Silva
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/367
• topic: Papillomaviridae; Neoplasias do Colo do Útero; HPV; Câncer de Colo Uterino; Ciências Biológicas

Cervical cancer is one of the biggest public health problems, being considered a major risk factor for women over 30 years, as chances of developing this disease caused by different factors, the most worrying being papilloma infection. Human virus (HPV) symptoms may be imperceptible in the early symptoms of the disease, but nowadays there are ways to prevent neoplasms with preventive tests, such as Oncotic Colpocytology. The specialized literature says that the morphological alteration of an HPV lesion, called low grade intraepithelial lesion (LSIL) is basically a koilocytotic halo, but a clinical practice that shows that koilocyte is not the only morphological pattern for if you detect an LSIL. Thus, this study aims to demonstrate the cellular changes of an LSIL that in clinical practice are also included in HSIL cells. Through the analysis of 150 fields from 32 slides of diagnosed oncotic colpocytology, a percentage of the 5 most frequently seen slide changes, respectively, were quantified as nuclear karyomegalies, koilocytotic halos, coarse plasma membranes, hyperchromatic nuclei and irregular nuclear membranes. Taking into account the amount of possible errors in the form, he suggests applying molecular biology tests such as in situ hybridization and PCR to reduce the false tests caused.