Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio

Detalhes bibliográficos
Autor(a) principal: Sloboda, Djeneffer Isabelle Ferreira
Data de Publicação: 2020
Outros Autores: Santos, Jaqueline Rosa dos
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório do Centro Universitário Braz Cubas
Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/3281
Resumo: Due to negative temperature, the freezing process of semen has adverse effects for the sperm cell such as loss of motility, vigor and increased morphological changes. With the addition of thinners and cryoprotectors this impact is decreased. Equex STM® is a surfactant added to thinners with the intention of decreasing osmotic shock and improving semen quality. The active ingredient of this product is sodium sulfate (DSS) detergent. Therefore, this study aims to analyze the effect of sodium sulfate dodecil (SSD) on sheep semen in glycine, yolk and milk thinner medium. Semen of 5 sheep was collected in four different moments, with an interval of one week. A semen pool divided into a control group composed of glycine thinner, yolk, milk and a treatment group was created with the addition of SSD to the same thinner. Factors such as whirlwind, motility, vigor and semen concentration were evaluated, in both groups, before freezing and after thawing to categorize the effect of detergent. After the experiment and statistical analysis it was concluded that the sodium sulfate dodecil added in glycine, yolk, milk showed no improvement in the quality of thawed sheep semen.
id CUB_2b5b495424ef33248f7664c5428a05cc
oai_identifier_str oai:repositorio.cruzeirodosul.edu.br:123456789/3281
network_acronym_str CUB
network_name_str Repositório do Centro Universitário Braz Cubas
repository_id_str
spelling Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódioCrioprotetorDiluidorDetergenteCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIADue to negative temperature, the freezing process of semen has adverse effects for the sperm cell such as loss of motility, vigor and increased morphological changes. With the addition of thinners and cryoprotectors this impact is decreased. Equex STM® is a surfactant added to thinners with the intention of decreasing osmotic shock and improving semen quality. The active ingredient of this product is sodium sulfate (DSS) detergent. Therefore, this study aims to analyze the effect of sodium sulfate dodecil (SSD) on sheep semen in glycine, yolk and milk thinner medium. Semen of 5 sheep was collected in four different moments, with an interval of one week. A semen pool divided into a control group composed of glycine thinner, yolk, milk and a treatment group was created with the addition of SSD to the same thinner. Factors such as whirlwind, motility, vigor and semen concentration were evaluated, in both groups, before freezing and after thawing to categorize the effect of detergent. After the experiment and statistical analysis it was concluded that the sodium sulfate dodecil added in glycine, yolk, milk showed no improvement in the quality of thawed sheep semen.-Devido a temperatura negativa, o processo de congelação do sêmen, possui efeitos adversos para a célula espermática como a perda de motilidade, vigor e aumento das alterações morfológicas. Com a adição de diluidores e crioprotetores esse impacto é diminuído. O Equex STM® é um surfactante adicionado a diluidores com a intenção de diminuir o choque osmótico e melhorar a qualidade do sêmen. O princípio ativo desse produto é o detergente dodecil sulfato de sódio(DSS). Por isso, esse estudo tem como objetivo principal analisar o efeito do dodecil sulfato de sódio(DSS) no sêmen ovino em meio diluidor de glicina, gema e leite. Foi coletado sêmen de 5 carneiros, em quatro momentos distintos, com intervalo de uma semana. Foi criado um pool de sêmen dividido em grupo controle composto por diluidor de glicina, gema, leite e um grupo tratamento com a adição do DSS ao mesmo diluidor. Fatores como turbilhonamento, motilidade, vigor e concentração do sêmen foram avaliados, em ambos os grupos, antes da congelação e após descongelamento para categorizar o efeito do detergente. Após o experimento e análise estatística conclui-se que o dodecil sulfato de sódio adicionado em meio de glicina, gema, leite não apresentou melhoria na qualidade do sêmen ovino descongelado.Universidade PositivoBrasilMedicina VeterináriaUPBergstein-Galan, Tacia G.http://lattes.cnpq.br/1201007653448641Sloboda, Djeneffer Isabelle FerreiraSantos, Jaqueline Rosa dos2022-03-23T18:33:11Z20222022-03-23T18:33:11Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/3281porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2022-03-23T18:35:31Zoai:repositorio.cruzeirodosul.edu.br:123456789/3281Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2022-03-23T18:35:31Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false
dc.title.none.fl_str_mv Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio
title Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio
spellingShingle Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio
Sloboda, Djeneffer Isabelle Ferreira
Crioprotetor
Diluidor
Detergente
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio
title_full Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio
title_fullStr Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio
title_full_unstemmed Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio
title_sort Criopreservação do sêmen ovino em diluente GGL adicionado dodecil sulfato de sódio
author Sloboda, Djeneffer Isabelle Ferreira
author_facet Sloboda, Djeneffer Isabelle Ferreira
Santos, Jaqueline Rosa dos
author_role author
author2 Santos, Jaqueline Rosa dos
author2_role author
dc.contributor.none.fl_str_mv Bergstein-Galan, Tacia G.
http://lattes.cnpq.br/1201007653448641
dc.contributor.author.fl_str_mv Sloboda, Djeneffer Isabelle Ferreira
Santos, Jaqueline Rosa dos
dc.subject.por.fl_str_mv Crioprotetor
Diluidor
Detergente
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
topic Crioprotetor
Diluidor
Detergente
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description Due to negative temperature, the freezing process of semen has adverse effects for the sperm cell such as loss of motility, vigor and increased morphological changes. With the addition of thinners and cryoprotectors this impact is decreased. Equex STM® is a surfactant added to thinners with the intention of decreasing osmotic shock and improving semen quality. The active ingredient of this product is sodium sulfate (DSS) detergent. Therefore, this study aims to analyze the effect of sodium sulfate dodecil (SSD) on sheep semen in glycine, yolk and milk thinner medium. Semen of 5 sheep was collected in four different moments, with an interval of one week. A semen pool divided into a control group composed of glycine thinner, yolk, milk and a treatment group was created with the addition of SSD to the same thinner. Factors such as whirlwind, motility, vigor and semen concentration were evaluated, in both groups, before freezing and after thawing to categorize the effect of detergent. After the experiment and statistical analysis it was concluded that the sodium sulfate dodecil added in glycine, yolk, milk showed no improvement in the quality of thawed sheep semen.
publishDate 2020
dc.date.none.fl_str_mv 2020
2022-03-23T18:33:11Z
2022
2022-03-23T18:33:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.cruzeirodosul.edu.br/handle/123456789/3281
url https://repositorio.cruzeirodosul.edu.br/handle/123456789/3281
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Positivo
Brasil
Medicina Veterinária
UP
publisher.none.fl_str_mv Universidade Positivo
Brasil
Medicina Veterinária
UP
dc.source.none.fl_str_mv reponame:Repositório do Centro Universitário Braz Cubas
instname:Centro Universitário Braz Cubas (CUB)
instacron:CUB
instname_str Centro Universitário Braz Cubas (CUB)
instacron_str CUB
institution CUB
reponame_str Repositório do Centro Universitário Braz Cubas
collection Repositório do Centro Universitário Braz Cubas
repository.name.fl_str_mv Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)
repository.mail.fl_str_mv bibli@brazcubas.edu.br
_version_ 1798311343121498112

Registros relacionados