Estudo comparativo da expressão do hormônio GLP1 entre os procedimentos de gastroplastia com desvio intestinal em Y de Roux e gastrectomia vertical em ratos Wistar

• Autor(a) principal: Ribeiro, André Richter
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2586
• topic: Cirurgia bariátrica; Obesidade; Técnicas operatórias; Biotecnologia industrial; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Obesity is a disease that affects millions of people throughout the world. Defined as overweight if above 20% the ideal weight, or Body Mass Index (BMI) above 30kg/m². In Brazil, this disease is expanding and affected, in 2008 and 2009, around 10% of the population in all regions of the country. From surgeries done to cure obesity and its comorbidities, concepts have been created that make us visualize the surgery today for the treatment of type-2 diabetes. The hormone action mechanisms of the obesity surgeries for the control of type-2 diabetes are not completely clear yet, for the improvement of the general condition of the patient the GLP-1 is involved, the hormone responsible for the remission of the condition. The goal of this study was to compare the expression of the GLP-1 after two bariatric surgery procedures. The GLP-1 is secreted by the L-cells which are produced in the intestines’ segments of the Wistar rats, similar to what happens in the human intestines. The procedures more commonly used in bariatric surgery on human patients are: the gastroplasty derivation with Roux-en-Y (GDYR), and the vertical gastrectomy, or sleeve, both adopted for this study. Eighteen Wistar rats have been operated, divided in 3 groups, being the first group submitted to a laparotomy, transection and intestine anastomosis; the second group has been undergone gastroplasty with derivation in Roux-en-Y (GDYR), and the third group was submitted to gastric sleeve (GV). The animals have been submitted to weigh and blood sugar measurements a day before the surgery, as well as in the third and seventh days after the surgery. On the seventh day after the surgery, the rats have been euthanized. An analytic and immune-histochemical study has been carried out on parts removed from the intestines, being 18 intestine segments from the ileum distal portion. The histological cuts have been destined for analysis with optic microscopy. Results: effects on weight reduction and blood sugar. The number of L-cells, determined by the GLP-1 expression through immune-histochemical analysis, was similar among the groups, therefore rejecting the hypothesis that the increased number of L-cells could be responsible for the increase of GLP-1 hormone levels after the GDYR and GV surgeries, and, consequently, to the reduction of the blood sugar.