Estudo da qualidade microbiológica da água do rio Passaúna, Curitiba, por meio do isolamento e análise de resistência da Escherichia coli a antibióticos

• Autor(a) principal: Silva, Trajano Felipe Barrabas Xavier da Silva
• Data de Publicação: 2007
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2323
• topic: Rio Passaúna; Água; Escherichia coli; Coliformes; Antibiótico; CNPQ::CIENCIAS BIOLOGICAS; CNPQ::ENGENHARIAS::ENGENHARIA SANITARIA

Supplying waters present a potencial risk of pollution either by wastewaters or by animal wastes of human or animal origin and, as a result, these waters may contain pathogenic microorganisms. For this, it is necessary to carry out microbiological analysis to determinate a security grade through the bacteriological view. Water analyses of Passaúna River – Curitiba/Paraná were carried out to determinate the contents of total and thermotolerant coliforms and also Escherichia coli, in order to obtain water quality indicatives. Samples were colleted monthly, from March 2006 to February 2007, and five different points of the river were analysed to obtain representative results for an extension of the river. In order to determinate the contents of total and thermotolerant coliforms the multiple tube method was performed (MPN/100mL), and the results obtained varied from 130 MPN/100mL to 1,6 x 106 MNP/100mL for total coliforms, where as for thermotoletant coliforms the variation was settled between 40 MPN/100mL and 5 x 105 MNP/100mL. The Escherichia coli strains isolated from the samples were tested with thirteen different antibiotics to determinate the antibiotic resistance. The results isolated strains showed that were constantly sensitive to seven of the thirteen analysed antibiotics, and also proved to be resistant to one of the other antibiotics. The results indicated that two factors can influence the increased contamination, which are sazonality and the domestic waste probable contamination. The determination of Antibiotic Resistance (AR) indices was carried out as a tool to provide information on the anthropogenic influence. Two analysed points were considered critical due to the anthropogenic influence, being responsible for lowering the soil permeability and for contaminating the river with organic compounds