Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais

Detalhes bibliográficos
Autor(a) principal: Vieira, Sandra Aparecida Luiz
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório do Centro Universitário Braz Cubas
Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/651
Resumo: This work aimed to compare the results obtained in the experimental research with low power laser, AlGa diode, 660nm wavelength, with different doses. 54 Wistar rats were used, divided into control groups, groups submitted to laser therapy at a dose of 4J / cm² and groups submitted to laser therapy at a dose of 8J / cm². Daily macroscopic evaluation of all animals was performed by pachymetry of skin wounds. For that, the animals were anesthetized via inhalation with halothane. The data obtained were analyzed by the Student's T test with 95% significance (p <0.05). All analyzes were performed using the GraphPad Prism 4.0 program. For histopathological examination, at 3, 7 and 14 days, the animals were euthanized on days 4, 8 and 15 after the experimental surgery. The lesion and surrounding areas were surgically removed, which were placed in 10% buffered formalin and identified. The material removed was processed, included in paraffin, cuts of 4 µm thick and stained with hematoxylin-eosin (HE). The samples were analyzed under light microscopy, observed and evaluated semi-quantitatively, inflammatory processes, degree of epithelialization, collagen fibers and edema. The number of new vessels was counted on a microscope grid in a standardized area of ​​0.25 mm2. The results of the macroscopic evaluation showed that 50% of the animals treated with laser therapy at a dose of 4J / cm² had total healing on the 12th day of treatment, and 83.3% of the animals had the same response on the 14th day. In the group of animals submitted to laser therapy at a dose of 8J / cm², only 16.6% of the animals had total healing on the 12th day of treatment, and 66.6% of the animals on the 14th day of treatment. Therefore, the lowest dose had the greatest ability to accelerate the healing process. The morphometric analysis of the cutaneous wound showed that, on the 7th day after the start of treatment, there was a statistically higher average number of new vessels (p <0.05) in animals treated with laser therapy at a dose of 4J / cm2, when compared to the control group. There were no significant differences between groups, control and 8J / cm2 in the same period. On the 14th day, no significant differences were observed between all groups evaluated. All animals, regardless of the group belonging, presented 100% of skin wounds re-epithelialization on the 14th day. This fact was probably due to the fact that the animal model used in this research had spontaneous healing in a relatively short period of time. The methodology used to evaluate edema, formation of collagen fibers and inflammatory infiltrate, was not able to detect significant differences between groups in the analyzed periods (3, 7 and 14 days). It is concluded that the radiation with low-power laser therapy, with a wavelength of 660nm, at doses of 4J / cm² and 8J / cm² did not present statistical differences between them. However, macroscopically there was a difference, in relation to total healing, between animals treated and those not treated with laser radiation. This work came to demonstrate the importance of using low power laser therapy in conditions that compromise the physical, psychological, social and economic integrity of a large number of human beings.
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spelling Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentaisEffect of low-power laser on the healing of experimental skin woundsCicatrizaçãoFerida cutâneaLaserterapiaCNPQ::CIENCIAS DA SAUDE::MEDICINA::RADIOLOGIA MEDICAThis work aimed to compare the results obtained in the experimental research with low power laser, AlGa diode, 660nm wavelength, with different doses. 54 Wistar rats were used, divided into control groups, groups submitted to laser therapy at a dose of 4J / cm² and groups submitted to laser therapy at a dose of 8J / cm². Daily macroscopic evaluation of all animals was performed by pachymetry of skin wounds. For that, the animals were anesthetized via inhalation with halothane. The data obtained were analyzed by the Student's T test with 95% significance (p <0.05). All analyzes were performed using the GraphPad Prism 4.0 program. For histopathological examination, at 3, 7 and 14 days, the animals were euthanized on days 4, 8 and 15 after the experimental surgery. The lesion and surrounding areas were surgically removed, which were placed in 10% buffered formalin and identified. The material removed was processed, included in paraffin, cuts of 4 µm thick and stained with hematoxylin-eosin (HE). The samples were analyzed under light microscopy, observed and evaluated semi-quantitatively, inflammatory processes, degree of epithelialization, collagen fibers and edema. The number of new vessels was counted on a microscope grid in a standardized area of ​​0.25 mm2. The results of the macroscopic evaluation showed that 50% of the animals treated with laser therapy at a dose of 4J / cm² had total healing on the 12th day of treatment, and 83.3% of the animals had the same response on the 14th day. In the group of animals submitted to laser therapy at a dose of 8J / cm², only 16.6% of the animals had total healing on the 12th day of treatment, and 66.6% of the animals on the 14th day of treatment. Therefore, the lowest dose had the greatest ability to accelerate the healing process. The morphometric analysis of the cutaneous wound showed that, on the 7th day after the start of treatment, there was a statistically higher average number of new vessels (p <0.05) in animals treated with laser therapy at a dose of 4J / cm2, when compared to the control group. There were no significant differences between groups, control and 8J / cm2 in the same period. On the 14th day, no significant differences were observed between all groups evaluated. All animals, regardless of the group belonging, presented 100% of skin wounds re-epithelialization on the 14th day. This fact was probably due to the fact that the animal model used in this research had spontaneous healing in a relatively short period of time. The methodology used to evaluate edema, formation of collagen fibers and inflammatory infiltrate, was not able to detect significant differences between groups in the analyzed periods (3, 7 and 14 days). It is concluded that the radiation with low-power laser therapy, with a wavelength of 660nm, at doses of 4J / cm² and 8J / cm² did not present statistical differences between them. However, macroscopically there was a difference, in relation to total healing, between animals treated and those not treated with laser radiation. This work came to demonstrate the importance of using low power laser therapy in conditions that compromise the physical, psychological, social and economic integrity of a large number of human beings.Este trabalho teve como objetivo comparar os resultados obtidos na pesquisa experimental com laser de baixa potência, diodo AlGa, comprimento de onda de 660nm, com diferentes doses. Utilizaram-se 54 ratos Wistar, divididos em grupos controle, grupos submetidos a laserterapia na dose de 4J/cm² e grupos submetidos a laserterapia na dose de 8J/cm². Realizou-se avaliação macroscópica diária, de todos os animais, pela paquimetria das feridas cutâneas. Para isso, os animais foram anestesiados via inalatória com halotano. Os dados obtidos foram analisados pelo teste T de Student com 95% de significância (p<0,05). Todas as análises foram realizadas com programa GraphPad Prism 4.0. Para o exame histopatológico, nos períodos de 3, 7 e 14 dias, os animais foram eutanasiados nos dias 4, 8 e 15 após a cirurgia experimental. Retirou-se cirurgicamente as áreas da lesão e circunjacente, que foram colocadas em formalina tamponada a 10% e identificadas. O material retirado foi processado, incluído em parafina, realizado cortes de 4µm de espessura e corado com hematoxilina-eosina (HE). As amostras foram analisadas à microscopia óptica de luz, observadas e avaliadas semi-quantitativamente, os processos inflamatórios, grau de epitelização, fibras de colágeno e edema. O número de neovasos foi contado em gratícula para microscópio em área padronizada de 0,25 mm2. Os resultados, da avaliação macroscópica mostraram que, 50% dos animais tratados com laserterapia na dose de 4J/cm² tiveram cicatrização total no 12o dia de tratamento, e 83,3% dos animais apresentaram a mesma resposta no 14ºdia. No grupo de animais submetidos a laserterapia na dose de 8J/cm², apenas 16,6% dos animais apresentaram cicatrização total no 12º dia de tratamento, e 66,6% dos animais no 14° dia de tratamento. Portanto, a menor dose teve maior capacidade de acelerar o processo de cicatrização. A análise morfométria da ferida cutânea demonstrou que, no 7ºdia após início do tratamento, havia número médio estatisticamente maior de neovasos (p<0,05) nos animais tratados com laserterapia na dose de 4J/cm2, quando comparado ao grupo controle. Não foram observadas diferenças significativas entre os grupos, controle e 8J/cm2 neste mesmo período. No 14o dia não foram observadas diferenças significativas entre todos os grupos avaliados. Todos os animais, independente do grupo pertencente, apresentaram 100% de reepitelização das feridas cutâneas ao 14o dia. Esse fato se deu, provavelmente, em razão do modelo animal utilizado nessa pesquisa ter cicatrização espontânea em um período de tempo relativamente curto. A metodologia empregada para avaliação do edema, formação de fibras colágenas e infiltrado inflamatório, não foi capaz de detectar diferenças significativas entre os grupos nos períodos analisados (3, 7 e 14 dias). Conclui-se que a radiação com laserterapia de baixa potência, com comprimento de onda de 660nm, nas doses de 4J/cm² e 8J/cm² não apresentaram diferenças estatísticas entre si. Porém, macroscopicamente houve diferença, em relação à cicatrização total, entre os animais tratados e os não tratados com radiação laser. Esse trabalho veio demonstrar a importância do uso da laserterapia de baixa potência em afecções que comprometem as integridades, física, psíquica, social e econômica de grande número de seres humanos.Universidade de FrancaBrasilPós-GraduaçãoPrograma de Mestrado em Promoção de SaúdeUNIFRANCastro, Márcio Botelho de2728337803011388http://lattes.cnpq.br/2728337803011388Bolzan, Aline Adriana8526181254357290http://lattes.cnpq.br/8526181254357290Nascif Júnior, Iucif Abrão7244827989359940http://lattes.cnpq.br/7244827989359940Vieira, Sandra Aparecida Luiz2020-05-11T20:51:26Z2020-05-11T20:51:26Z2006-06-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfVIEIRA, Sandra Aparecida Luiz. Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais. Franca, 2006. 49 f. Dissertação (Mestrado em Promoção de Saúde) - Universidade de Franca. 2006.https://repositorio.cruzeirodosul.edu.br/handle/123456789/651porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2020-05-11T20:52:31Zoai:repositorio.cruzeirodosul.edu.br:123456789/651Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2020-05-11T20:52:31Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false
dc.title.none.fl_str_mv Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais
Effect of low-power laser on the healing of experimental skin wounds
title Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais
spellingShingle Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais
Vieira, Sandra Aparecida Luiz
Cicatrização
Ferida cutânea
Laserterapia
CNPQ::CIENCIAS DA SAUDE::MEDICINA::RADIOLOGIA MEDICA
title_short Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais
title_full Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais
title_fullStr Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais
title_full_unstemmed Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais
title_sort Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais
author Vieira, Sandra Aparecida Luiz
author_facet Vieira, Sandra Aparecida Luiz
author_role author
dc.contributor.none.fl_str_mv Castro, Márcio Botelho de
2728337803011388
http://lattes.cnpq.br/2728337803011388
Bolzan, Aline Adriana
8526181254357290
http://lattes.cnpq.br/8526181254357290
Nascif Júnior, Iucif Abrão
7244827989359940
http://lattes.cnpq.br/7244827989359940
dc.contributor.author.fl_str_mv Vieira, Sandra Aparecida Luiz
dc.subject.por.fl_str_mv Cicatrização
Ferida cutânea
Laserterapia
CNPQ::CIENCIAS DA SAUDE::MEDICINA::RADIOLOGIA MEDICA
topic Cicatrização
Ferida cutânea
Laserterapia
CNPQ::CIENCIAS DA SAUDE::MEDICINA::RADIOLOGIA MEDICA
description This work aimed to compare the results obtained in the experimental research with low power laser, AlGa diode, 660nm wavelength, with different doses. 54 Wistar rats were used, divided into control groups, groups submitted to laser therapy at a dose of 4J / cm² and groups submitted to laser therapy at a dose of 8J / cm². Daily macroscopic evaluation of all animals was performed by pachymetry of skin wounds. For that, the animals were anesthetized via inhalation with halothane. The data obtained were analyzed by the Student's T test with 95% significance (p <0.05). All analyzes were performed using the GraphPad Prism 4.0 program. For histopathological examination, at 3, 7 and 14 days, the animals were euthanized on days 4, 8 and 15 after the experimental surgery. The lesion and surrounding areas were surgically removed, which were placed in 10% buffered formalin and identified. The material removed was processed, included in paraffin, cuts of 4 µm thick and stained with hematoxylin-eosin (HE). The samples were analyzed under light microscopy, observed and evaluated semi-quantitatively, inflammatory processes, degree of epithelialization, collagen fibers and edema. The number of new vessels was counted on a microscope grid in a standardized area of ​​0.25 mm2. The results of the macroscopic evaluation showed that 50% of the animals treated with laser therapy at a dose of 4J / cm² had total healing on the 12th day of treatment, and 83.3% of the animals had the same response on the 14th day. In the group of animals submitted to laser therapy at a dose of 8J / cm², only 16.6% of the animals had total healing on the 12th day of treatment, and 66.6% of the animals on the 14th day of treatment. Therefore, the lowest dose had the greatest ability to accelerate the healing process. The morphometric analysis of the cutaneous wound showed that, on the 7th day after the start of treatment, there was a statistically higher average number of new vessels (p <0.05) in animals treated with laser therapy at a dose of 4J / cm2, when compared to the control group. There were no significant differences between groups, control and 8J / cm2 in the same period. On the 14th day, no significant differences were observed between all groups evaluated. All animals, regardless of the group belonging, presented 100% of skin wounds re-epithelialization on the 14th day. This fact was probably due to the fact that the animal model used in this research had spontaneous healing in a relatively short period of time. The methodology used to evaluate edema, formation of collagen fibers and inflammatory infiltrate, was not able to detect significant differences between groups in the analyzed periods (3, 7 and 14 days). It is concluded that the radiation with low-power laser therapy, with a wavelength of 660nm, at doses of 4J / cm² and 8J / cm² did not present statistical differences between them. However, macroscopically there was a difference, in relation to total healing, between animals treated and those not treated with laser radiation. This work came to demonstrate the importance of using low power laser therapy in conditions that compromise the physical, psychological, social and economic integrity of a large number of human beings.
publishDate 2006
dc.date.none.fl_str_mv 2006-06-24
2020-05-11T20:51:26Z
2020-05-11T20:51:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv VIEIRA, Sandra Aparecida Luiz. Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais. Franca, 2006. 49 f. Dissertação (Mestrado em Promoção de Saúde) - Universidade de Franca. 2006.
https://repositorio.cruzeirodosul.edu.br/handle/123456789/651
identifier_str_mv VIEIRA, Sandra Aparecida Luiz. Efeito do laser de baixa potência na cicatrização de feridas cutâneas experimentais. Franca, 2006. 49 f. Dissertação (Mestrado em Promoção de Saúde) - Universidade de Franca. 2006.
url https://repositorio.cruzeirodosul.edu.br/handle/123456789/651
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de Franca
Brasil
Pós-Graduação
Programa de Mestrado em Promoção de Saúde
UNIFRAN
publisher.none.fl_str_mv Universidade de Franca
Brasil
Pós-Graduação
Programa de Mestrado em Promoção de Saúde
UNIFRAN
dc.source.none.fl_str_mv reponame:Repositório do Centro Universitário Braz Cubas
instname:Centro Universitário Braz Cubas (CUB)
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instname_str Centro Universitário Braz Cubas (CUB)
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institution CUB
reponame_str Repositório do Centro Universitário Braz Cubas
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repository.name.fl_str_mv Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)
repository.mail.fl_str_mv bibli@brazcubas.edu.br
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