Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório do Centro Universitário Braz Cubas |
Texto Completo: | https://repositorio.cruzeirodosul.edu.br/handle/123456789/2134 |
Resumo: | The objective of this study was to evaluate the association of genetic polymorphisms in RUNX2, BMP4, BMP2, TGFβ1, EGF, SMAD6, ESR1, ESR2 and PTH with permanent tooth size (TS) variations. This cross-sectional study was carried out with 110 individuals of both sexes. Dental casts were used to measure the maximum crown dimensions of all fully erupted permanent teeth (except third molars) in the mesiodistal (MD) and buccolingual (BL) width. The DNA was obtained from the epithelial cells of the oral mucosa and the genetic polymorphisms of RUNX2 (rs59983488 and rs1200425), BMP4 (rs17563), BMP2 (rs235768 and rs1005464), TGFβ1 (rs1800470), EGF (rs4444903), SMAD6 (rs2119261 and rs3934908), ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), and PTH (rs694, rs307247 and rs6256) were genotyped by real-time PCR. The data were submitted to statistical analysis with a significance level of 0.05. The genetic polymorphisms rs59983488, rs1200425, rs17563, rs235768, rs1005464, rs1800470, rs4444903, rs2234693, rs9340799, rs1256049 and rs4986938, rs694, rs307247 were associated with MD and BL TS in the maxillary and mandibular arch (p < 0.05). The polymorphism rs2119261 was associated with variation in TS only in the upper teeth (p < 0.05). In rs6256, there was an association only in the BL width of the upper left canine (p < 0.05). The rs3934908 was not associated with any TS (p > 0.05). Finally, this study showed that there is an association between variation in TS with genetic polymorphisms in RUNX2, EGF, TGFβ1, BMP2, BMP4, SMAD6, PTH, ESR1, and ESR2 indicating a possible role of these genes in tooth development. |
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Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentesOdontologia clínicaDenteDentição permanenteGenéticaOdontogênesePolimorfismo genéticoCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAThe objective of this study was to evaluate the association of genetic polymorphisms in RUNX2, BMP4, BMP2, TGFβ1, EGF, SMAD6, ESR1, ESR2 and PTH with permanent tooth size (TS) variations. This cross-sectional study was carried out with 110 individuals of both sexes. Dental casts were used to measure the maximum crown dimensions of all fully erupted permanent teeth (except third molars) in the mesiodistal (MD) and buccolingual (BL) width. The DNA was obtained from the epithelial cells of the oral mucosa and the genetic polymorphisms of RUNX2 (rs59983488 and rs1200425), BMP4 (rs17563), BMP2 (rs235768 and rs1005464), TGFβ1 (rs1800470), EGF (rs4444903), SMAD6 (rs2119261 and rs3934908), ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), and PTH (rs694, rs307247 and rs6256) were genotyped by real-time PCR. The data were submitted to statistical analysis with a significance level of 0.05. The genetic polymorphisms rs59983488, rs1200425, rs17563, rs235768, rs1005464, rs1800470, rs4444903, rs2234693, rs9340799, rs1256049 and rs4986938, rs694, rs307247 were associated with MD and BL TS in the maxillary and mandibular arch (p < 0.05). The polymorphism rs2119261 was associated with variation in TS only in the upper teeth (p < 0.05). In rs6256, there was an association only in the BL width of the upper left canine (p < 0.05). The rs3934908 was not associated with any TS (p > 0.05). Finally, this study showed that there is an association between variation in TS with genetic polymorphisms in RUNX2, EGF, TGFβ1, BMP2, BMP4, SMAD6, PTH, ESR1, and ESR2 indicating a possible role of these genes in tooth development.O objetivo desse trabalho foi avaliar a associação de polimorfismos genéticos do RUNX2, BMP4, BMP2, TGFβ1, EGF, SMAD6, ESR1, ESR2 e PTH com variações de tamanho de dentes permanentes. Este estudo transversal foi realizado com 110 indivíduos de ambos os sexos. Os modelos de gesso foram utilizados para medir as máximas dimensões da coroa de todos os dentes permanentes totalmente irrompidos (exceto terceiros molares) na direção mésio-distal (MD) e vestíbulo-lingual (VL). O DNA foi obtido a partir das células epiteliais da mucosa oral e os polimorfismos genéticos do RUNX2 (rs59983488 e rs1200425), BMP4 (rs17563), BMP2 (rs235768 e rs1005464), TGFβ1 (rs1800470), EGF (rs4444903), SMAD6 (rs2119261 e rs3934908), ESR1 (rs2234693 e rs9340799), ESR2 (rs1256049 e rs4986938) e PTH (rs694, rs307247 e rs6256), foram genotipados por PCR em tempo real. Os dados foram submetidos à análise estatística com nível de significância de 0,05. Os polimorfismos genéticos rs59983488, rs1200425, rs17563, rs235768, rs1005464, rs1800470, rs4444903, rs2234693, rs9340799, rs1256049 e rs4986938, rs694, rs307247 foram associados a medidas dentárias MD e VL, tanto na arcada superior quanto na inferior (p < 0,05). O polimorfismo rs2119261 foi associado a variação do tamanho dentário somente nos dentes superiores (p < 0,05). No rs6256, houve associação na medida VL do canino superior esquerdo (p < 0,05). O marcador rs3934908 não foi associado a nenhuma medida dentária (p > 0,05). Por fim, este estudo mostrou que existe uma associação entre a variação no tamanho dentário com polimorfismos genéticos no RUNX2, EGF, TGFβ1, BMP2, BMP4, SMAD6, PTH, ESR1 e ESR2 indicando um possível papel desses genes no desenvolvimento dentário.Universidade PositivoBrasilPós-GraduaçãoPrograma de Pós-Graduação em Odontologia ClínicaUPScariot, Rafaelahttp://lattes.cnpq.br/8726711027143249Küchler, Erika Calvanohttp://lattes.cnpq.br/1395527593517316Gerber, Jennifer Tsi2021-05-11T21:09:21Z20202021-05-11T21:09:21Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://repositorio.cruzeirodosul.edu.br/handle/123456789/2134porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2021-06-17T18:27:49Zoai:repositorio.cruzeirodosul.edu.br:123456789/2134Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2021-06-17T18:27:49Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false |
dc.title.none.fl_str_mv |
Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes |
title |
Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes |
spellingShingle |
Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes Gerber, Jennifer Tsi Odontologia clínica Dente Dentição permanente Genética Odontogênese Polimorfismo genético CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes |
title_full |
Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes |
title_fullStr |
Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes |
title_full_unstemmed |
Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes |
title_sort |
Avaliação de genes candidatos a variações do tamanho mésio-distal e vestíbulo-lingual de dentes permanentes |
author |
Gerber, Jennifer Tsi |
author_facet |
Gerber, Jennifer Tsi |
author_role |
author |
dc.contributor.none.fl_str_mv |
Scariot, Rafaela http://lattes.cnpq.br/8726711027143249 Küchler, Erika Calvano http://lattes.cnpq.br/1395527593517316 |
dc.contributor.author.fl_str_mv |
Gerber, Jennifer Tsi |
dc.subject.por.fl_str_mv |
Odontologia clínica Dente Dentição permanente Genética Odontogênese Polimorfismo genético CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
topic |
Odontologia clínica Dente Dentição permanente Genética Odontogênese Polimorfismo genético CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The objective of this study was to evaluate the association of genetic polymorphisms in RUNX2, BMP4, BMP2, TGFβ1, EGF, SMAD6, ESR1, ESR2 and PTH with permanent tooth size (TS) variations. This cross-sectional study was carried out with 110 individuals of both sexes. Dental casts were used to measure the maximum crown dimensions of all fully erupted permanent teeth (except third molars) in the mesiodistal (MD) and buccolingual (BL) width. The DNA was obtained from the epithelial cells of the oral mucosa and the genetic polymorphisms of RUNX2 (rs59983488 and rs1200425), BMP4 (rs17563), BMP2 (rs235768 and rs1005464), TGFβ1 (rs1800470), EGF (rs4444903), SMAD6 (rs2119261 and rs3934908), ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), and PTH (rs694, rs307247 and rs6256) were genotyped by real-time PCR. The data were submitted to statistical analysis with a significance level of 0.05. The genetic polymorphisms rs59983488, rs1200425, rs17563, rs235768, rs1005464, rs1800470, rs4444903, rs2234693, rs9340799, rs1256049 and rs4986938, rs694, rs307247 were associated with MD and BL TS in the maxillary and mandibular arch (p < 0.05). The polymorphism rs2119261 was associated with variation in TS only in the upper teeth (p < 0.05). In rs6256, there was an association only in the BL width of the upper left canine (p < 0.05). The rs3934908 was not associated with any TS (p > 0.05). Finally, this study showed that there is an association between variation in TS with genetic polymorphisms in RUNX2, EGF, TGFβ1, BMP2, BMP4, SMAD6, PTH, ESR1, and ESR2 indicating a possible role of these genes in tooth development. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020 2021-05-11T21:09:21Z 2021-05-11T21:09:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2134 |
url |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2134 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Odontologia Clínica UP |
publisher.none.fl_str_mv |
Universidade Positivo Brasil Pós-Graduação Programa de Pós-Graduação em Odontologia Clínica UP |
dc.source.none.fl_str_mv |
reponame:Repositório do Centro Universitário Braz Cubas instname:Centro Universitário Braz Cubas (CUB) instacron:CUB |
instname_str |
Centro Universitário Braz Cubas (CUB) |
instacron_str |
CUB |
institution |
CUB |
reponame_str |
Repositório do Centro Universitário Braz Cubas |
collection |
Repositório do Centro Universitário Braz Cubas |
repository.name.fl_str_mv |
Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB) |
repository.mail.fl_str_mv |
bibli@brazcubas.edu.br |
_version_ |
1798311337825140736 |