Utilização do extrato de descarte do nácar do mexilhão Perna perna para o desenvolvimento de biomateriais com potencial aplicação como enxerto ósseo

• Autor(a) principal: Silva, Rosiellen da
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2550
• topic: Biomateriais; Enxertos ósseos; Nácar; Perna perna; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Tissue engineering requires materials with characteristics matching those of human bone, to provide efficiency and low cost, the repair of bone defects. In an attempt to meet this need and reduce environmental impacts generated by products aimed to use the disposal of nacre extract of Perna perna mussels development of biomaterials with potential osteo promoter. To this end, the nacre of the mussel above was particulate, with the result of this process analyzed for light microscopy, fluorescence spectrometry X-ray energy dispersive; thermogravimetry and differential scanning calorimetry, assessment tests in dry heat and moist; extraction of the organic content, which was assessed by thin layer chromatography, and spectrophotometry. The results showed the feasibility of the nacre particles the shell, and the presence of protein as a constituent thereof being obtained by extracting protein solubility in acetic acid or EDTA. In vitro, the presence of the organic extract protein, in combination with chloride salts of calcium, sodium phosphate, and sodium bicarbonate, caused an increase in the mass of crystallization and crystallization points, suggesting a potential use for bone grafting. As for the scaffolds developed for bone repair, the organic extract and / or particulate from the nacre of the shell associated with alginate, glycerol and water, they also showed promise as a reparation for dental and bone reconstruction.