Avaliação das diferenças funcionais e estruturais do couro cabeludo

• Autor(a) principal: França, Karinna Siqueira Machado de
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2609
• topic: Biotecnologia; Couro cabeludo; Identificação biométrica; Eficácia; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

The increasing demand for cosmetic products that offer benefits has been increasing over time, and this growth can be seen in the huge range of products launched annually by the hair care market. For the registration of cosmetic products in Brazil, studies are required to ensure the consumer's safety in the use of the product as well as the proof of each attribute offered in the packaging, and ANVISA demands the results of the safety and efficacy tests, respectively. Since the evaluations of efficacy of extreme importance in this process, techniques, combined with the analysis of images and histological evaluations through biopsies, are alternatives for proving attributes of hair cosmetic products. The objective was to verify the performance of different methodologies for evaluation of parameters related to the scalp, and observing if the selected techniques can contribute to the discrimination the results in study groups. The evaluation of structural and functional parameters was considered in his region in a study of 75 female volunteers who were selected and divided into age, sebaceous (oily and non-greasy scalp) and chemical exposure (n = 15 volunteers per assessed group). After preparation of the scalp with demarcation in the vertex of the head, biometric parameters evaluations such as hydration, skin barrier, oiliness, pH and temperature were carried out by instrumental techniques. Skin roughness was also assessed by imaging techniques, and histological evaluations were performed on biopsied material, which was subjected to quantification of filaggrin and labeling of the ki67 and β-catenin proteins by immunofluorescence. The results indicated that there was no significant difference for the parameters hydration, pH, roughness, thermography and filaggrin evaluation among the evaluated groups. A statistical difference was observed for the evaluation of the barrier function, both in terms of age and exposure to chemical treatments. The oil evaluation reported a difference between the groups regarding sebaceous production. It was also observed a statistical difference in temperature evaluation, both between ages and in relation to exposure to chemical treatments. The results of the biopsies reported a higher labeling of β-catenin and ki67 in the group exposed to chemical treatments. Based on the results obtained, it is concluded that the methodologies evaluated in this study are important tools that offer the opportunity to explore the physiology and structural properties of the scalp.