Estudo fitoquímico e avaliação das atividades esquistossomicida, antimicrobiana e acetilcolinesterásica de Roupala montana Aubl. (Proteaceae)

• Autor(a) principal: Souza, Herbert Cristian de
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/517
• topic: Proteaceae; Roupala montana; Estudo fitoquímico; Atividade esquistossomicida; Antimicrobiana; Acetilcolinesterásica; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA

Roupala montana is a species of the family Proteaceae with very few or almost no reports on the phytochemical profile and / or biological studies, which makes this original research. This work focuses on the preparation of a crude extract of aerial parts of Roupala montana, followed by fractionation with different solvents of increasing polarity, yielding hexane fractions (RP1), Ethyl acetate (RP2), n-butanol ( RP3) and hydromethanol (P4), and their respective biological assays (schistosomicidal, antimicrobial and acetilcolinesterásica). The fractionation, the fractions were monitored (RP1, RP2, RP3 and RP4) by chromatographic assays by High Performance Liquid Chromatography (HPLC), which was selected for chromatographic procedures RP1 fraction by GC-MS trace its objective phytochemical profile. Through the phytochemical study of RP1 fraction by GC-MS allowed the identification of two (2) diterpenes (Phytol and Neofitadieno), 3 (three) triterpenes (β-amyrin, α-amyrin and lupeol), two (2) carotenóis (γ-tocopherol and δtocopherol) and 1 (a) ester of fatty acid (palmitic acid ethyl ester), as major constituents. The potential schistosomicidal crude extract (RP) and the fractions from the liquid-liquid partition was evaluated in vitro against the adult worms of Schistosoma mansoni. The results indicate that the P2 fraction was the most active among the samples tested, this being promoted 100% death of the parasites on the dose of 50 mg / mL in the fifth day of treatment. Additionally, the results obtained against the antimicrobial activity of crude extract and fractions Roupala montana showed MIC values intermediate, and only a fraction RP2 weakly inhibited the growth of Streptococcus pneumoniae, with MIC of 200 mg / mL, which could suggest its use as an aid in the control and reduction of pathogenic microorganisms. In addition, the action acetilcolinesterasica of Roupala montana fractions revealed that RP1, RP2 and RP3 had a slight potential to inhibit this enzyme front, and had a white spot at the same retention factor (Rf).