Efeitos da leptina e SAA na função de neutrófilos humanos

Detalhes bibliográficos
Autor(a) principal: Silva, Bianca Palmeira Santos da
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório do Centro Universitário Braz Cubas
Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2666
Resumo: Although there is a consensus that the infections incidence is higher in obese, the effect of adipokines on neutrophil function is not completely known. Herein, we hypothesized that leptin and serum amyloid A (SAA) can activate/inactivate neutrophils and interfere with the innate immune response. So, we studied the combined effects of leptin and SAA on the function of human neutrophils. Neutrophils (2.5x106 cells / mL) were isolated and cultured in RPMI medium in the presence and absence of leptin (25 and 50ng/mL), SAA (5ug/mL) and LPS (1ug/mL). In vitro, we analyzed, through flow cytometry technique, the effect of leptin and SAA on parameters related to neutrophil viability; by ELISA we evaluated the release of cytokines (TNF-α, IL-8) and by the chemiluminescence technique amplified by lucigenin we observed the production of ROS. Under the conditions studied, neutrophil treatments with SAA, leptin and the combination SAA and leptin were not toxic to the cells. We observed an increase in ROS production of 60% (*p= 0.01) and 79% (**p= 0.02) by neutrophils, when treated with the combination of SAA and leptin at concentrations of 2.5ng/mL and 25ng/mL respectively. The treatment of neutrophils with leptin at concentrations of 25ng/mL, 50ng/mL and SAA increased by 38% (**p= 0.02) and 92% (***p= 0.003) and 159% (****p= 0.0002) the concentration of TNF-α and these cells, when treated, with leptin at concentrations of 25ng/mL and 50ng/mL with SAA showed an increase of 62% (°p= 0.0044), and 106% (°°°p= 0.0002), respectively. The treatment of neutrophils with leptin at concentrations 25ng / mL and 50ng/mL and SAA increased by 39% (**p= 0.01), 38% (***p= 0.01) and 62% (****p= 0.001) the concentration of IL-8, respectively, and these neutrophils when treated with leptin at a concentration of 50ng / mL with SAA showed an increase of 32% (°p= 0.0001). There was an increase in the production of TNF-α and IL-8 at different concentrations of leptin and in treatments with combined leptin and SAA. Our data indicate that adipose tissue can modulate neutrophil function through adipokine secretion and interfere with the innate immune response.
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spelling Efeitos da leptina e SAA na função de neutrófilos humanosEffects of leptin and SAA on function of human neutrophilsLeucócitosObesidadeInflamação.CNPQ::CIENCIAS DA SAUDEAlthough there is a consensus that the infections incidence is higher in obese, the effect of adipokines on neutrophil function is not completely known. Herein, we hypothesized that leptin and serum amyloid A (SAA) can activate/inactivate neutrophils and interfere with the innate immune response. So, we studied the combined effects of leptin and SAA on the function of human neutrophils. Neutrophils (2.5x106 cells / mL) were isolated and cultured in RPMI medium in the presence and absence of leptin (25 and 50ng/mL), SAA (5ug/mL) and LPS (1ug/mL). In vitro, we analyzed, through flow cytometry technique, the effect of leptin and SAA on parameters related to neutrophil viability; by ELISA we evaluated the release of cytokines (TNF-α, IL-8) and by the chemiluminescence technique amplified by lucigenin we observed the production of ROS. Under the conditions studied, neutrophil treatments with SAA, leptin and the combination SAA and leptin were not toxic to the cells. We observed an increase in ROS production of 60% (*p= 0.01) and 79% (**p= 0.02) by neutrophils, when treated with the combination of SAA and leptin at concentrations of 2.5ng/mL and 25ng/mL respectively. The treatment of neutrophils with leptin at concentrations of 25ng/mL, 50ng/mL and SAA increased by 38% (**p= 0.02) and 92% (***p= 0.003) and 159% (****p= 0.0002) the concentration of TNF-α and these cells, when treated, with leptin at concentrations of 25ng/mL and 50ng/mL with SAA showed an increase of 62% (°p= 0.0044), and 106% (°°°p= 0.0002), respectively. The treatment of neutrophils with leptin at concentrations 25ng / mL and 50ng/mL and SAA increased by 39% (**p= 0.01), 38% (***p= 0.01) and 62% (****p= 0.001) the concentration of IL-8, respectively, and these neutrophils when treated with leptin at a concentration of 50ng / mL with SAA showed an increase of 32% (°p= 0.0001). There was an increase in the production of TNF-α and IL-8 at different concentrations of leptin and in treatments with combined leptin and SAA. Our data indicate that adipose tissue can modulate neutrophil function through adipokine secretion and interfere with the innate immune response.Embora seja consenso que a incidência e o agravamento de infeções são maiores em obesos, o efeito de adipocinas, mediadores secretados pelo tecido adiposo, como a amilóide sérica A (SAA) e a leptina, na função de neutrófilos, primeira linha de defesa do organismo, não é completamente conhecido. Acreditando na hipótese de que a leptina e a SAA possam ativar/inativar neutrófilos e interferir na resposta imune inata, nesse trabalho estudamos os efeitos da leptina e de leptina e SAA na função de neutrófilos humanos. Neutrófilos (2,5x106 células/mL) foram isolados do sangue periférico de doadores saudáveis, e posteriormente, cultivados em meio RPMI na presença de leptina (25 e 50ng/mL), SAA (5ug/mL) e leptina nas diferentes concentrações em conjunto com a SAA. LPS (1ug/mL) foi utilizado como controle positivo. In vitro analisamos, através da técnica de citometria de fluxo o efeito da leptina e de SAA sobre parâmetros relacionados à viabilidade de neutrófilos; por ELISA avaliamos a liberação de TNF-α e IL-8 e pela técnica de quimiluminescência amplificada por lucigenina observamos a produção de ROS. Nas condições estudadas, os tratamentos de neutrófilos com SAA, leptina e a combinação de ambas não foram tóxicos para as células. Observamos aumento na produção de ROS de 60% (*p=0.01) e 79% (**p=0.02) por neutrófilos, quando tratados com a combinação de SAA e leptina nas concentrações de 2.5ng/mL e 25ng/mL respectivamente. O tratamento de neutrófilos com leptina nas concentrações de 25ng/mL, 50ng/mL e SAA isoladamente aumentou 38% (**p=0.02), 92% (***p=0.003) e 159% (****p=0.0002) a concentração de TNF-α, respectivamente e essas células, quando tratadas com leptina nas concentrações de 25ng/mL e 50ng/mL em conjunto com a SAA apresentaram aumento de 62% (°p=0.0044), e 106% (°°°p=0.0002), respectivamente. A concentração de IL-8 foi aumentada em neutrófilos tradados com leptina nas concentrações 25ng/mL e 50ng/mL e SAA isoladamente, 39% (**p=0.01), 38% (***p= 0.01) e 62% (****p=0.001), respectivamente e essas células quando tratados com leptina na concentração 50ng/mL em conjunto com a SAA apresentaram aumento de 32% (°p=0.0001). Os resultados indicam que a combinação de leptina e SAA induz a produção de ROS, TNF-α e IL-8 por neutrófilos. Os nossos dados apontam que o tecido adiposo pode através da secreção de adipocinas modular a função de neutrófilos e interferir na resposta imune inata.Universidade Cruzeiro do SulBrasilPrograma de Pós Graduação em Ciências da SaúdeCruzeiro do SulDermargos, Elaine Hatanaka18316791838http://lattes.cnpq.br/0256403741529064Dermargos, Elaine Hatanaka18316791838http://lattes.cnpq.br/0256403741529064Gorjão , Renatahttp://lattes.cnpq.br/6211339830408691Sandri, Silvanahttp://lattes.cnpq.br/3978430910797029Silva, Bianca Palmeira Santos da2021-08-20T16:58:30Z2020-05-072021-08-20T16:58:30Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfPALMEIRA B. Efeitos da leptina e da SAA na função de neutrófilos humano. 58 f. 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dc.title.none.fl_str_mv Efeitos da leptina e SAA na função de neutrófilos humanos
Effects of leptin and SAA on function of human neutrophils
title Efeitos da leptina e SAA na função de neutrófilos humanos
spellingShingle Efeitos da leptina e SAA na função de neutrófilos humanos
Silva, Bianca Palmeira Santos da
Leucócitos
Obesidade
Inflamação.
CNPQ::CIENCIAS DA SAUDE
title_short Efeitos da leptina e SAA na função de neutrófilos humanos
title_full Efeitos da leptina e SAA na função de neutrófilos humanos
title_fullStr Efeitos da leptina e SAA na função de neutrófilos humanos
title_full_unstemmed Efeitos da leptina e SAA na função de neutrófilos humanos
title_sort Efeitos da leptina e SAA na função de neutrófilos humanos
author Silva, Bianca Palmeira Santos da
author_facet Silva, Bianca Palmeira Santos da
author_role author
dc.contributor.none.fl_str_mv Dermargos, Elaine Hatanaka
18316791838
http://lattes.cnpq.br/0256403741529064
Dermargos, Elaine Hatanaka
18316791838
http://lattes.cnpq.br/0256403741529064
Gorjão , Renata
http://lattes.cnpq.br/6211339830408691
Sandri, Silvana
http://lattes.cnpq.br/3978430910797029
dc.contributor.author.fl_str_mv Silva, Bianca Palmeira Santos da
dc.subject.por.fl_str_mv Leucócitos
Obesidade
Inflamação.
CNPQ::CIENCIAS DA SAUDE
topic Leucócitos
Obesidade
Inflamação.
CNPQ::CIENCIAS DA SAUDE
description Although there is a consensus that the infections incidence is higher in obese, the effect of adipokines on neutrophil function is not completely known. Herein, we hypothesized that leptin and serum amyloid A (SAA) can activate/inactivate neutrophils and interfere with the innate immune response. So, we studied the combined effects of leptin and SAA on the function of human neutrophils. Neutrophils (2.5x106 cells / mL) were isolated and cultured in RPMI medium in the presence and absence of leptin (25 and 50ng/mL), SAA (5ug/mL) and LPS (1ug/mL). In vitro, we analyzed, through flow cytometry technique, the effect of leptin and SAA on parameters related to neutrophil viability; by ELISA we evaluated the release of cytokines (TNF-α, IL-8) and by the chemiluminescence technique amplified by lucigenin we observed the production of ROS. Under the conditions studied, neutrophil treatments with SAA, leptin and the combination SAA and leptin were not toxic to the cells. We observed an increase in ROS production of 60% (*p= 0.01) and 79% (**p= 0.02) by neutrophils, when treated with the combination of SAA and leptin at concentrations of 2.5ng/mL and 25ng/mL respectively. The treatment of neutrophils with leptin at concentrations of 25ng/mL, 50ng/mL and SAA increased by 38% (**p= 0.02) and 92% (***p= 0.003) and 159% (****p= 0.0002) the concentration of TNF-α and these cells, when treated, with leptin at concentrations of 25ng/mL and 50ng/mL with SAA showed an increase of 62% (°p= 0.0044), and 106% (°°°p= 0.0002), respectively. The treatment of neutrophils with leptin at concentrations 25ng / mL and 50ng/mL and SAA increased by 39% (**p= 0.01), 38% (***p= 0.01) and 62% (****p= 0.001) the concentration of IL-8, respectively, and these neutrophils when treated with leptin at a concentration of 50ng / mL with SAA showed an increase of 32% (°p= 0.0001). There was an increase in the production of TNF-α and IL-8 at different concentrations of leptin and in treatments with combined leptin and SAA. Our data indicate that adipose tissue can modulate neutrophil function through adipokine secretion and interfere with the innate immune response.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-07
2020
2021-08-20T16:58:30Z
2021-08-20T16:58:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv PALMEIRA B. Efeitos da leptina e da SAA na função de neutrófilos humano. 58 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Cruzeiro do Sul, São Paulo, 2020.
https://repositorio.cruzeirodosul.edu.br/handle/123456789/2666
identifier_str_mv PALMEIRA B. Efeitos da leptina e da SAA na função de neutrófilos humano. 58 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Cruzeiro do Sul, São Paulo, 2020.
url https://repositorio.cruzeirodosul.edu.br/handle/123456789/2666
dc.language.iso.fl_str_mv por
language por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Cruzeiro do Sul
Brasil
Programa de Pós Graduação em Ciências da Saúde
Cruzeiro do Sul
publisher.none.fl_str_mv Universidade Cruzeiro do Sul
Brasil
Programa de Pós Graduação em Ciências da Saúde
Cruzeiro do Sul
dc.source.none.fl_str_mv reponame:Repositório do Centro Universitário Braz Cubas
instname:Centro Universitário Braz Cubas (CUB)
instacron:CUB
instname_str Centro Universitário Braz Cubas (CUB)
instacron_str CUB
institution CUB
reponame_str Repositório do Centro Universitário Braz Cubas
collection Repositório do Centro Universitário Braz Cubas
repository.name.fl_str_mv Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)
repository.mail.fl_str_mv bibli@brazcubas.edu.br
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