Análise quantitativa e qualitativa da microbiota oral de grandes felídeos de cativeiro e perfil de susceptibilidade antimicrobiana do hidrocloreto de polihexametileno guanidina

• Autor(a) principal: Barros, Renata Alves
• Data de Publicação: 2022
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/4141
• topic: Animais selvagens - Medicina; Microbiologia; Sistema estomatognático; Vida terrestre; Polímero sintético; Antisséptico; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA

The present study aimed to quantify and identify the oral microbiota of six large captive felids from Bosque Municipal Dr. Fábio Barreto (Ribeirão Preto, SP) and to evaluate the “in vitro” antimicrobial susceptibility profile of bacterial isolates against polyhexamethylene guanidine hydrochloride polymer, compared to chlorhexidine digluconate, intending its use as a mouthwash in dental procedures. For this, oral samples were collected from four specimens of Puma concolor, a Panthera onca and a Panthera leo, counting the colony forming units per milliliter (CFU/mL), followed by semiautomatic identification. As for the antimicrobial susceptibility profile, the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and bactericidal kinetics of the polymer were determined. The result were expressed in a descriptive way. The samples collected showed relevant amounts of bacterial growth, with a total identification of 16 bacterial isolates, six of which were Gram- positive and 10 Gram-negative. There was growth of eight bacterial isolates of P. concolor (four Gram-positive and four Gram-negative); two from P. onca (Gram-negative) and six from P. leo (two Gram-positive and four Gram-negative). The PHMGH showed MIC and CBM values ranging from 0.24 to 125.00 μg/mL, being lower than those of CLX against three isolates. Regarding bactericidal kinetics, PHMGH reduced the microbial load above 90% for all microorganisms within 30 minutes, differing from CLX, and only two Gram-positive isolates exposed to the polymer did not show complete elimination after 60 minutes. Of contact. The quantitative-qualitative data may help in the prevention and treatment of oral diseases. PHMGH showed promising potential, in low concentrations and, in a short period of time, compared to the commercial product chlorhexidine digluconate, which makes it a future option of active ingredient for mouthwash for veterinary use. Keywords: wildlife medicine, microbiology, stomatognathic system, terrestrial life, synthetic polymer, antiseptic.