Análise imunohistoquímica do P16INK4A, KI-67 e HLA-G em neoplasias intraepiteliais cervicais e no câncer de colo uterino

• Autor(a) principal: Fin, Fabio Roberto
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2610
• topic: Biotecnologia; Colo uterino - Câncer; Neoplasia intraepitelial cervical; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Persistent infection with human papillomavirus (HPV) is the main risk factor for cervical cancer and its precursor lesions. However only a small portion of women with HPV will develop dysplasia and high-grade cervical cancer. Based on this carcinogenesis, the identification of new tumor markers which allow to monitor events associated with tumor progression may improve the detection of lesions with greater risk to progress. The objective of this study is to analyze the expression of three markers, p16INK4a, Ki-67 and HLA-G in cervical lesions with low dysplasia and high-grade, in patients with carcinoma, and in women with cervical uninjured. A retrospective study of 70 women divided into four groups: I: 10 women without cervical lesions; II: 15 with low-grade dysplasia; III: 22 with high-grade dysplasia; IV: 23 with carcinoma. These biopsies were assessed by immunohistochemical studies by means of expression of p16INK4a protein, HLA-G and the Ki 67. Results: regarding the positivity of p16INK4a, 21/22 (95.5%) of patients with high dysplasia degree and 21/23 (91.3%) with cervical cancer had a positive p16INK4a 2+, and found only 4/15 (26.7%) of patients with low-grade dysplasia (p <0.001). For the Ki-67 marker positivity was statistically significant for the groups III and IV (13/22 and 15/23 respectively) compared with the group I and II (0/10 and 4/15 respectively). Regarding to HLA-G label there was no expression in all samples. Conclusions: The immunohistochemical markers p16INK4a and Ki-67 as present in cervical biopsies are associated with increased likelihood of developing.