Efeito da idade e do alendronato de sódio no processo de reparo ósseo de fraturas: estudo bioquímico e mecânico em fêmur de ratos

• Autor(a) principal: Bonetto, Luana Mordask
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2076
• topic: Odontologia; Periodonto - Regeneração; Fraturas; Ossos; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

The objective of this study was to evaluate the effect of age and sodium alendronate on the bone repair of femoral fractures of rats, by means of serum levels of calcium and alkaline phosphatase levels and the evaluation of crosssection and flexural strength of the femurs.Wistar rats (n = 40) were allocated into four groups: group O (control middle-age rats), group Y (control young rats), group OA (middle-age rats who received 1 mg/kg alendronate) and group YA (young rats who received 1 mg/kg of alendronate). The rats underwent linear fracture surgery followed by stable internal fixation with a 2.0 mm bolt and plates system. The animals in groups OA and YA received alendronate sodium applications three times a week, while the control group received 0.9% saline solution. Drug administration was performed until euthanasia at 90 days. 1 ml of blood was collected from the animals at 3 times: before surgery, 45 days and 90 days after the procedure for biochemical analysis of the serum levels of calcium and alkaline phosphatase. After euthanasia, the femurs fractured and not fractured were removed and the three-point bending tests were performed. The data were submitted to statistical and descriptive analysis with significance level of 0.05. According to the biochemical analysis of blood, there was no difference in serum calcium and alkaline phosphatase levels evaluated at the same time between the groups and evaluated over time in the same group (p > 0.05). Regarding the cross section of femurs on the fractured and non-fractured side, the young animals presented a lower cross section than middle aged animals (p < 0.05). Only on the fractured side, young animals presented greater flexural strength than middle aged animals (p < 0.05). There was no difference between the animals that used or not alendronate in relation to the cross-section and flexural strength (p > 0.05). When compared to the fractured and non-fractured femurs of the same animal, it is possible to observe a greater cross section of femurs on the fractured side (p < 0.05) when compared to the non-fractured side. The flexural strength presented higher values in femurs on the nonfractured side (p < 0.05). There was a strong positive correlation between weight and cross section (R = + 0.91) and moderate to strong negative correlation between weight and flexural strength of the fractured and nonfractured side, respectively (R = -0.97 and R = -0.71). In conclusion, there was no difference in the biochemical analysis of the serum concentration of calcium and alkaline phosphatase during the bone repair process. Regarding mechanical analysis, age was shown to influence the cross section and the flexural strength of the animals, unlike alendronate that showed no association with these factors.