Efeitos da proporção de anticoagulante, tempo e temperatura de estocagem sobre a estabilidade hematológica de bovinos, equinos, ovinos e suínos

• Autor(a) principal: Silva, Maria Fernanda da
• Data de Publicação: 2021
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2708
• topic: Análise laboratorial; Animais de produção; Erros pré-analíticos; Estabilidade; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA

Field veterinarians are constantly challenged to minimize pre-analytical errors in hematological examinations of farm animals. Considering the variables that commonly affect laboratory analyzes in this category, the objective was to assess the stability of the complete blood count with differential leukocyte counts in cattle, horses, sheep and swine. For this, 6 animals of each species were selected, collected with a total capacity and with average capacity of the tube to evaluate the stability in different proportions of EDTAK3 / blood, storage temperature (environment (25°C) and refrigeration 4 a 8°C)) and at different times (0h, 12h, 24h and 48h). The complete blood count was performed using an automatic counter and the differential leukocyte count manually, and the data were compared by ANOVA. The hematocrit value increased in pigs in pigs with total capacity and mean of blood kept at room temperature for 48 hours. Hematimetric indices also suffered external oscillations in sheep, pigs and horses from 24 hours of room temperature and refrigeration. Platelets and total leukocytes in medium capacity decreased without time 48 hours at room temperature and 24 hours for refrigerated sheep. It is concluded that the parameters anticoagulant composition / blood, temperature and time can interfere in the analyses, highlighting the need to know the interspecies changes in order to avoid untrue results. KEYWORDS: laboratory analysis, pre-analytical errors, production animals, stability