Caracterização de células-tronco derivadas de lipoaspirado quanto ao potencial de diferenciação, marcadores imunofenotípicos e genes específicos

• Autor(a) principal: Radoski, Caroline Leal
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2561
• topic: Adipócito; Células-tronco mesenquimais; Terapia celular; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Stem cells have the ability to self-renew and differentiate into various cell lines. For this reason, several research groups have been studying the therapeutic potential of these cells in regeneration of bone and cartilage, breast reconstruction, autoimmune diseases, among other applications. Stem cells derived from mesenchymal tissues can differentiate into osteoblastic lineage, chondrogenic and adipogenic, being present in many body tissues, like bone marrow and adipose tissue. Adipose tissue provides a great source of stem cells that can be collected in large quantities with increased yield compared to bone marrow. So we sought in this study to isolate the cell population obtained from human lipoaspirate, as well as observing the behavior of these cells in culture, tests for phenotypic characterization, cellular differentiation and gene expression. The isolated cells had a fibroblastoid shape, and the immunophenotypic profile described in the literature for mesenchymal stem cells and also differentiated into three cell lines. We analyzed the gene expression of cells isolated at different cell passage, noting differences in gene expression pattern over time. As a result, some genes had their expression modulated in a passagedependent manner. In conclusion, it was possible to isolate and characterize this cell lines when different passages were analyzed.