Avaliação da atividade de diterpenos frente a bactérias multirresistentes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório do Centro Universitário Braz Cubas |
Texto Completo: | https://repositorio.cruzeirodosul.edu.br/handle/123456789/516 |
Resumo: | In the last years, microbial resistance to the multiple drugs, as well as the appearance of new types of infections is increasing rapidly and becoming a work-wide public health problem (Gaspar-Marques et al. 2006; Spellberg et al., 2004) because they are infectious diseases a major cause of morbidity and mortality in developed and developing countries (Ahmad & Beg, 2001). Before the appearance of infections with microorganisms resistant strains to conventional chemotherapy, the scientific community has been over the years focusing to find a new sources of substances to be use against multiresistant bacteria. One of the main sources of these substances are natural products, by isolation of essential metabolites for its maintenance, which are commonly applied in several studies of biological activity. Among these metabolites used, the diterpenes has shown promising results, individually or as enhancers of other substances present on the market. Natural products biosynthesized from mevalonic acid through the pyrophosphate 2E, 6E, 10E-geranilgeranila (GGPP) (Dewick, 2002). According to the number of rings and the cyclization pattern of their chemical structures, diterpenes are divided into acyclic, bicyclic, tricyclic, tetracyclic, and mixed macrocyclic (Garcia et al, 2007; Hanson, 2004). The dissertation in question sought to isolate through Viguiera arenaria strata, Copaifera langsdorffii and Mikania glomerata diterpenes ent-pimara-8 (14) ,15-dien-19-oic acid, ent8 (14)-3β ,15-pimaradien -ol, ent-cowrie-16 (17)-en-19-oic acid and ent-8 (17)-13Elabdadieno-15-oic acid, which after the isolation were subjected to analysis of 13C NMR (Nuclear Magnetic Resonance Nuclear Carbon 13) and 1H NMR (Nuclear Magnetic Resonance of Hydrogen) to determinate their chemical structure. After elucidated the diterpenes chemical structures mentioned above, its potential has been tested front of bacterias Staphylococcus aureus - IC WB81 USA 400, Staphylococcus aureus - 180 HCRP, Staphylococcus aureus - ATCC 29213, Staphylococcus aureus - IC W7749 USA 200, Staphylococcus capitis - ATCC 27840 , Staphylococcus capitis - 207 HCRP, Staphylococcus epidermis - ATCC 14990, Staphylococcus epidermis - 177 HCRP, Staphylococcus haemolyticus - 213 HCRP, Staphylococcus haemolyticus ATCC 29970, Streptococcus pneumoniae – 176 HCRP, Enterococcus faecalis - 179 HCRP, Enterococcus faecalis - NCTC 775, Enterococcus faecium - NCTC 717 in the Laboratory Research in Applied Microbiology (LAPEMA - Unifran), under the supervision of Professor. Dr. Carlos Henrique Gomes Martins. During the determination of minimum inhibitory concentration, we found promising results against a panel of multiresistant bacteria of relevant clinical importance based on the values proposed by Ríos & Recio. |
id |
CUB_fe9b0dd36754c8e37113346503a25e4e |
---|---|
oai_identifier_str |
oai:repositorio.cruzeirodosul.edu.br:123456789/516 |
network_acronym_str |
CUB |
network_name_str |
Repositório do Centro Universitário Braz Cubas |
repository_id_str |
|
spelling |
Avaliação da atividade de diterpenos frente a bactérias multirresistentesEvaluation of diterpene activity against multidrug-resistant bacteriaQuímica - Produtos naturais (Viguiera arenaria)Copaifera langsdorffii - Mikania glomerata, diterpenosÁcido ent-pimara-8(14), 15-dien-19-óicoEnt-8(14),15-pimaradien-3ß-olÁcido ent-caur-16(17)-en-19-óico; ácido ent-8(17)-13e-labdadieno-15-óicoAtividade biológica; bactéria multirresistentesCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAIn the last years, microbial resistance to the multiple drugs, as well as the appearance of new types of infections is increasing rapidly and becoming a work-wide public health problem (Gaspar-Marques et al. 2006; Spellberg et al., 2004) because they are infectious diseases a major cause of morbidity and mortality in developed and developing countries (Ahmad & Beg, 2001). Before the appearance of infections with microorganisms resistant strains to conventional chemotherapy, the scientific community has been over the years focusing to find a new sources of substances to be use against multiresistant bacteria. One of the main sources of these substances are natural products, by isolation of essential metabolites for its maintenance, which are commonly applied in several studies of biological activity. Among these metabolites used, the diterpenes has shown promising results, individually or as enhancers of other substances present on the market. Natural products biosynthesized from mevalonic acid through the pyrophosphate 2E, 6E, 10E-geranilgeranila (GGPP) (Dewick, 2002). According to the number of rings and the cyclization pattern of their chemical structures, diterpenes are divided into acyclic, bicyclic, tricyclic, tetracyclic, and mixed macrocyclic (Garcia et al, 2007; Hanson, 2004). The dissertation in question sought to isolate through Viguiera arenaria strata, Copaifera langsdorffii and Mikania glomerata diterpenes ent-pimara-8 (14) ,15-dien-19-oic acid, ent8 (14)-3β ,15-pimaradien -ol, ent-cowrie-16 (17)-en-19-oic acid and ent-8 (17)-13Elabdadieno-15-oic acid, which after the isolation were subjected to analysis of 13C NMR (Nuclear Magnetic Resonance Nuclear Carbon 13) and 1H NMR (Nuclear Magnetic Resonance of Hydrogen) to determinate their chemical structure. After elucidated the diterpenes chemical structures mentioned above, its potential has been tested front of bacterias Staphylococcus aureus - IC WB81 USA 400, Staphylococcus aureus - 180 HCRP, Staphylococcus aureus - ATCC 29213, Staphylococcus aureus - IC W7749 USA 200, Staphylococcus capitis - ATCC 27840 , Staphylococcus capitis - 207 HCRP, Staphylococcus epidermis - ATCC 14990, Staphylococcus epidermis - 177 HCRP, Staphylococcus haemolyticus - 213 HCRP, Staphylococcus haemolyticus ATCC 29970, Streptococcus pneumoniae – 176 HCRP, Enterococcus faecalis - 179 HCRP, Enterococcus faecalis - NCTC 775, Enterococcus faecium - NCTC 717 in the Laboratory Research in Applied Microbiology (LAPEMA - Unifran), under the supervision of Professor. Dr. Carlos Henrique Gomes Martins. During the determination of minimum inhibitory concentration, we found promising results against a panel of multiresistant bacteria of relevant clinical importance based on the values proposed by Ríos & Recio.Nos últimos anos, a resistência microbiana às múltiplas drogas, bem como o aparecimento de novos tipos de infecções, vem aumentando rapidamente e tornando-se um problema mundial de saúde pública (Gaspar-Marques et al., 2006; Spellberg et al., 2004), por serem as doenças infecciosas uma das principais causas de morbidade e mortalidade nos países desenvolvidos e em desenvolvimento (Ahmad & Beg, 2001). Diante do aparecimento de infecções com cepas de microrganismos resistentes aos quimioterápicos convencionais, a comunidade cientifica vem ao longo dos anos concentrando esforços na busca de novas fontes de substâncias para serem usadas contra bactérias multirresistentes. Uma das principais fontes de obtenção dessas substâncias são os produtos naturais, através do isolamento de metabólitos essenciais para sua manutenção, os quais são comumente aplicados em vários estudos de atividade biológica. Dentre esses metabólitos usados, os diterpenos têm-se mostrado promissores, tanto individualmente quanto potencializadores de outras substâncias já presentes no mercado. Os produtos naturais biosintetizados a partir do ácido mevalônico, através do pirofosfato de 2E, 6E, 10E-geranilgeranila (GGPP) (Dewick, 2002). De acordo com o número de anéis e o padrão de ciclização de suas estruturas químicas, os diterpenos são divididos em acíclicos, bicíclicos, tricíclicos, tetracíclicos, macrocíclicos e mistos (Garcia et al, 2007; Hanson, 2004). O trabalho em questão visou isolar, através de estratos de Viguiera arenaria, Copaifera langsdorffii e Mikania glomerata, os diterpenos ácido ent-pimara-8(14), 15-dien-19-óico, ent-8(14), 15-pimaradien-3β-ol, ácido ent-caur-16(17)-en-19-óico e ácido ent-8(17)-13Elabdadieno-15-óico que, após terminado o isolamento, os diterpenos citados foram submetidos à análises de RMN 13C (Ressonância Magnética Nuclear de Carbono 13) e RMN 1H (Ressonância Magnética Nuclear de Hidrogênio) para a determinação da sua estrutura química. Depois de elucidada a estrutura química dos diterpenos citados acima, foi testada seu potencial frente às bactérias Staphylococcus aureus – IC WB81 USA 400, Staphylococcus aureus – 180 HCRP, Staphylococcus aureus – ATCC 29213, Staphylococcus aureus – IC W7749 USA 200, Staphylococcus capitis – ATCC 27840, Staphylococcus capitis – 207 HCRP, Staphylococcus epidermides – ATCC 14990, Staphylococcus epidermides – 177 HCRP, Staphylococcus haemolyticus – 213 HCRP, Staphylococcus haemolyticus ATCC 29970, Streptococcus pneumoniae – 176 HCRP, Enterococcus faecalis – 179 HCRP, Enterococcus faecalis – NCTC 775, Enterococcus faecium – NCTC 717 no Laboratório de Pesquisa em Microbiologia Aplicada (LAPEMA – UNIFRAN), sob supervisão do Prof. Dr. Carlos Henrique Gomes Martins. Durante a determinação da concentração inibitória mínima, observou-se resultados promissores contra um painel de bactérias multirresistentes de relevante importância clinica baseado nos valores propostos por Rios & Récio.Universidade de FrancaBrasilPós-GraduaçãoPrograma de Mestrado em CiênciasUNIFRANAmbrósio, Sérgio Ricardo7942579499577578http://lattes.cnpq.br/7942579499577578Gregório, Luiz Elídio8267826459967218http://lattes.cnpq.br/8267826459967218Heleno, Vladimir Constantino Gomes8691255594471749http://lattes.cnpq.br/8691255594471749Silva, José Rodolfo Macedo Souza da2020-04-14T15:59:52Z2020-04-14T15:59:52Z2011-06-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfSILVA, José Rodolfo Macedo Souza da. Avaliação da atividade de diterpenos frente a bactérias multirresistentes. Franca, 2011. 70 f. Dissertação (Mestrado em Ciências) - Universidade de Franca. 2011.https://repositorio.cruzeirodosul.edu.br/handle/123456789/516porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2020-04-14T16:00:57Zoai:repositorio.cruzeirodosul.edu.br:123456789/516Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2020-04-14T16:00:57Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false |
dc.title.none.fl_str_mv |
Avaliação da atividade de diterpenos frente a bactérias multirresistentes Evaluation of diterpene activity against multidrug-resistant bacteria |
title |
Avaliação da atividade de diterpenos frente a bactérias multirresistentes |
spellingShingle |
Avaliação da atividade de diterpenos frente a bactérias multirresistentes Silva, José Rodolfo Macedo Souza da Química - Produtos naturais (Viguiera arenaria) Copaifera langsdorffii - Mikania glomerata, diterpenos Ácido ent-pimara-8(14), 15-dien-19-óico Ent-8(14),15-pimaradien-3ß-ol Ácido ent-caur-16(17)-en-19-óico; ácido ent-8(17)-13e-labdadieno-15-óico Atividade biológica; bactéria multirresistentes CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Avaliação da atividade de diterpenos frente a bactérias multirresistentes |
title_full |
Avaliação da atividade de diterpenos frente a bactérias multirresistentes |
title_fullStr |
Avaliação da atividade de diterpenos frente a bactérias multirresistentes |
title_full_unstemmed |
Avaliação da atividade de diterpenos frente a bactérias multirresistentes |
title_sort |
Avaliação da atividade de diterpenos frente a bactérias multirresistentes |
author |
Silva, José Rodolfo Macedo Souza da |
author_facet |
Silva, José Rodolfo Macedo Souza da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ambrósio, Sérgio Ricardo 7942579499577578 http://lattes.cnpq.br/7942579499577578 Gregório, Luiz Elídio 8267826459967218 http://lattes.cnpq.br/8267826459967218 Heleno, Vladimir Constantino Gomes 8691255594471749 http://lattes.cnpq.br/8691255594471749 |
dc.contributor.author.fl_str_mv |
Silva, José Rodolfo Macedo Souza da |
dc.subject.por.fl_str_mv |
Química - Produtos naturais (Viguiera arenaria) Copaifera langsdorffii - Mikania glomerata, diterpenos Ácido ent-pimara-8(14), 15-dien-19-óico Ent-8(14),15-pimaradien-3ß-ol Ácido ent-caur-16(17)-en-19-óico; ácido ent-8(17)-13e-labdadieno-15-óico Atividade biológica; bactéria multirresistentes CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
topic |
Química - Produtos naturais (Viguiera arenaria) Copaifera langsdorffii - Mikania glomerata, diterpenos Ácido ent-pimara-8(14), 15-dien-19-óico Ent-8(14),15-pimaradien-3ß-ol Ácido ent-caur-16(17)-en-19-óico; ácido ent-8(17)-13e-labdadieno-15-óico Atividade biológica; bactéria multirresistentes CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
In the last years, microbial resistance to the multiple drugs, as well as the appearance of new types of infections is increasing rapidly and becoming a work-wide public health problem (Gaspar-Marques et al. 2006; Spellberg et al., 2004) because they are infectious diseases a major cause of morbidity and mortality in developed and developing countries (Ahmad & Beg, 2001). Before the appearance of infections with microorganisms resistant strains to conventional chemotherapy, the scientific community has been over the years focusing to find a new sources of substances to be use against multiresistant bacteria. One of the main sources of these substances are natural products, by isolation of essential metabolites for its maintenance, which are commonly applied in several studies of biological activity. Among these metabolites used, the diterpenes has shown promising results, individually or as enhancers of other substances present on the market. Natural products biosynthesized from mevalonic acid through the pyrophosphate 2E, 6E, 10E-geranilgeranila (GGPP) (Dewick, 2002). According to the number of rings and the cyclization pattern of their chemical structures, diterpenes are divided into acyclic, bicyclic, tricyclic, tetracyclic, and mixed macrocyclic (Garcia et al, 2007; Hanson, 2004). The dissertation in question sought to isolate through Viguiera arenaria strata, Copaifera langsdorffii and Mikania glomerata diterpenes ent-pimara-8 (14) ,15-dien-19-oic acid, ent8 (14)-3β ,15-pimaradien -ol, ent-cowrie-16 (17)-en-19-oic acid and ent-8 (17)-13Elabdadieno-15-oic acid, which after the isolation were subjected to analysis of 13C NMR (Nuclear Magnetic Resonance Nuclear Carbon 13) and 1H NMR (Nuclear Magnetic Resonance of Hydrogen) to determinate their chemical structure. After elucidated the diterpenes chemical structures mentioned above, its potential has been tested front of bacterias Staphylococcus aureus - IC WB81 USA 400, Staphylococcus aureus - 180 HCRP, Staphylococcus aureus - ATCC 29213, Staphylococcus aureus - IC W7749 USA 200, Staphylococcus capitis - ATCC 27840 , Staphylococcus capitis - 207 HCRP, Staphylococcus epidermis - ATCC 14990, Staphylococcus epidermis - 177 HCRP, Staphylococcus haemolyticus - 213 HCRP, Staphylococcus haemolyticus ATCC 29970, Streptococcus pneumoniae – 176 HCRP, Enterococcus faecalis - 179 HCRP, Enterococcus faecalis - NCTC 775, Enterococcus faecium - NCTC 717 in the Laboratory Research in Applied Microbiology (LAPEMA - Unifran), under the supervision of Professor. Dr. Carlos Henrique Gomes Martins. During the determination of minimum inhibitory concentration, we found promising results against a panel of multiresistant bacteria of relevant clinical importance based on the values proposed by Ríos & Recio. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-16 2020-04-14T15:59:52Z 2020-04-14T15:59:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, José Rodolfo Macedo Souza da. Avaliação da atividade de diterpenos frente a bactérias multirresistentes. Franca, 2011. 70 f. Dissertação (Mestrado em Ciências) - Universidade de Franca. 2011. https://repositorio.cruzeirodosul.edu.br/handle/123456789/516 |
identifier_str_mv |
SILVA, José Rodolfo Macedo Souza da. Avaliação da atividade de diterpenos frente a bactérias multirresistentes. Franca, 2011. 70 f. Dissertação (Mestrado em Ciências) - Universidade de Franca. 2011. |
url |
https://repositorio.cruzeirodosul.edu.br/handle/123456789/516 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de Franca Brasil Pós-Graduação Programa de Mestrado em Ciências UNIFRAN |
publisher.none.fl_str_mv |
Universidade de Franca Brasil Pós-Graduação Programa de Mestrado em Ciências UNIFRAN |
dc.source.none.fl_str_mv |
reponame:Repositório do Centro Universitário Braz Cubas instname:Centro Universitário Braz Cubas (CUB) instacron:CUB |
instname_str |
Centro Universitário Braz Cubas (CUB) |
instacron_str |
CUB |
institution |
CUB |
reponame_str |
Repositório do Centro Universitário Braz Cubas |
collection |
Repositório do Centro Universitário Braz Cubas |
repository.name.fl_str_mv |
Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB) |
repository.mail.fl_str_mv |
bibli@brazcubas.edu.br |
_version_ |
1798311359749816320 |