Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Dental Press Journal of Orthodontics |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512016000500039 |
Resumo: | ABSTRACT Objective: To evaluate and compare in vitro cytotoxicity and corrosion resistance of mini-implants from three different commercial brands used for orthodontic anchorage. Methods: Six mini-implants (Conexão(tm), Neodent(tm) and SIN(tm)) were separately immersed in artificial saliva (pH 6.76) for 30 and 60 days. The cytotoxicity of the corrosion extracts was assessed in L929 cell cultures using the violet crystal and MTT assays, as well as cell morphology under light microscopy. Metal surface characteristics before and after immersion in artificial saliva were assessed by means of scanning electron microscopy (SEM). The samples underwent atomic absorption spectrophotometry to determine the concentrations of aluminum and vanadium ions, constituent elements of the alloy that present potential toxicity. For statistical analysis, one-way ANOVA/Bonferroni tests were used for comparisons among groups with p < 0.05 considered significant. Statistical analysis was carried out with Graph Pad PRISM software Version 4.0. Results: No changes in cell viability or morphology were observed. Mini-implants SEM images revealed smooth surfaces with no obvious traces of corrosion. The extracts assessed by means of atomic absorption spectrophotometry presented concentrations of aluminum and vanadium ions below 1.0 µg/mL and 0.5 µg/mL, respectively. Conclusion: Orthodontic mini-implants manufactured by Conexão(tm), Neodent(tm) and SIN(tm) present high corrosion resistance and are not cytotoxic. |
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Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implantsMini-implantsCorrosionCytotoxicityABSTRACT Objective: To evaluate and compare in vitro cytotoxicity and corrosion resistance of mini-implants from three different commercial brands used for orthodontic anchorage. Methods: Six mini-implants (Conexão(tm), Neodent(tm) and SIN(tm)) were separately immersed in artificial saliva (pH 6.76) for 30 and 60 days. The cytotoxicity of the corrosion extracts was assessed in L929 cell cultures using the violet crystal and MTT assays, as well as cell morphology under light microscopy. Metal surface characteristics before and after immersion in artificial saliva were assessed by means of scanning electron microscopy (SEM). The samples underwent atomic absorption spectrophotometry to determine the concentrations of aluminum and vanadium ions, constituent elements of the alloy that present potential toxicity. For statistical analysis, one-way ANOVA/Bonferroni tests were used for comparisons among groups with p < 0.05 considered significant. Statistical analysis was carried out with Graph Pad PRISM software Version 4.0. Results: No changes in cell viability or morphology were observed. Mini-implants SEM images revealed smooth surfaces with no obvious traces of corrosion. The extracts assessed by means of atomic absorption spectrophotometry presented concentrations of aluminum and vanadium ions below 1.0 µg/mL and 0.5 µg/mL, respectively. Conclusion: Orthodontic mini-implants manufactured by Conexão(tm), Neodent(tm) and SIN(tm) present high corrosion resistance and are not cytotoxic.Dental Press International2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512016000500039Dental Press Journal of Orthodontics v.21 n.5 2016reponame:Dental Press Journal of Orthodonticsinstname:Dental Press International (DPI)instacron:DPI10.1590/2177-6709.21.5.039-046.oarinfo:eu-repo/semantics/openAccessAlves,Celha Borges CostaSegurado,Márcio NunesDorta,Miriam Cristina LeandroDias,Fátima RibeiroLenza,Maurício GuilhermeLenza,Marcos Augustoeng2017-01-30T00:00:00Zoai:scielo:S2176-94512016000500039Revistahttp://www.scielo.br/dpjoONGhttps://old.scielo.br/oai/scielo-oai.phpartigos@dentalpress.com.br||davidnormando@hotmail.com2177-67092176-9451opendoar:2017-01-30T00:00Dental Press Journal of Orthodontics - Dental Press International (DPI)false |
dc.title.none.fl_str_mv |
Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants |
title |
Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants |
spellingShingle |
Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants Alves,Celha Borges Costa Mini-implants Corrosion Cytotoxicity |
title_short |
Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants |
title_full |
Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants |
title_fullStr |
Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants |
title_full_unstemmed |
Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants |
title_sort |
Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants |
author |
Alves,Celha Borges Costa |
author_facet |
Alves,Celha Borges Costa Segurado,Márcio Nunes Dorta,Miriam Cristina Leandro Dias,Fátima Ribeiro Lenza,Maurício Guilherme Lenza,Marcos Augusto |
author_role |
author |
author2 |
Segurado,Márcio Nunes Dorta,Miriam Cristina Leandro Dias,Fátima Ribeiro Lenza,Maurício Guilherme Lenza,Marcos Augusto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Alves,Celha Borges Costa Segurado,Márcio Nunes Dorta,Miriam Cristina Leandro Dias,Fátima Ribeiro Lenza,Maurício Guilherme Lenza,Marcos Augusto |
dc.subject.por.fl_str_mv |
Mini-implants Corrosion Cytotoxicity |
topic |
Mini-implants Corrosion Cytotoxicity |
description |
ABSTRACT Objective: To evaluate and compare in vitro cytotoxicity and corrosion resistance of mini-implants from three different commercial brands used for orthodontic anchorage. Methods: Six mini-implants (Conexão(tm), Neodent(tm) and SIN(tm)) were separately immersed in artificial saliva (pH 6.76) for 30 and 60 days. The cytotoxicity of the corrosion extracts was assessed in L929 cell cultures using the violet crystal and MTT assays, as well as cell morphology under light microscopy. Metal surface characteristics before and after immersion in artificial saliva were assessed by means of scanning electron microscopy (SEM). The samples underwent atomic absorption spectrophotometry to determine the concentrations of aluminum and vanadium ions, constituent elements of the alloy that present potential toxicity. For statistical analysis, one-way ANOVA/Bonferroni tests were used for comparisons among groups with p < 0.05 considered significant. Statistical analysis was carried out with Graph Pad PRISM software Version 4.0. Results: No changes in cell viability or morphology were observed. Mini-implants SEM images revealed smooth surfaces with no obvious traces of corrosion. The extracts assessed by means of atomic absorption spectrophotometry presented concentrations of aluminum and vanadium ions below 1.0 µg/mL and 0.5 µg/mL, respectively. Conclusion: Orthodontic mini-implants manufactured by Conexão(tm), Neodent(tm) and SIN(tm) present high corrosion resistance and are not cytotoxic. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512016000500039 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512016000500039 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2177-6709.21.5.039-046.oar |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Dental Press International |
publisher.none.fl_str_mv |
Dental Press International |
dc.source.none.fl_str_mv |
Dental Press Journal of Orthodontics v.21 n.5 2016 reponame:Dental Press Journal of Orthodontics instname:Dental Press International (DPI) instacron:DPI |
instname_str |
Dental Press International (DPI) |
instacron_str |
DPI |
institution |
DPI |
reponame_str |
Dental Press Journal of Orthodontics |
collection |
Dental Press Journal of Orthodontics |
repository.name.fl_str_mv |
Dental Press Journal of Orthodontics - Dental Press International (DPI) |
repository.mail.fl_str_mv |
artigos@dentalpress.com.br||davidnormando@hotmail.com |
_version_ |
1754122397668081664 |