Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia).
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Conjunto de dados |
Título da fonte: | Repositório de Dados de Pesquisa da EMBRAPA (Redape) |
Texto Completo: | https://doi.org/10.48432/39ZANH |
Resumo: | Biotechnology-Based Assets – Saline stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia), ready to be cloned into a binary vector and later validated in a model plant or target plant to verify the ability to confer salinity tolerance by overexpression. The transcriptomics databases (RNASeq) of Portulaca oleracea (Beldroega) and Gliricidia sepium (Gliricidia), delivered to the Embrapa Agroenergia Pre-Technology Showcase in 2019 and 2020, underwent a selection process to identify saline stress-responsive genes. The databases were within the scope of the SI/CI "Establish a database of phenomics, genomics, transcriptomics and metabolomics of Palm Oil, focusing on the generation of information related to the response of this culture to abiotic and biotic stresses." The selection process led to 298 genes in purslane and 85 in gliricidia. This set of 383 saline stress-responsive genes constitutes a collection of Biotechnology-Based Assets to be validated for their ability to confer tolerance to this abiotic stress. Initially, we developed new versions of the Reference Transcripts (TR) of these two species using their respective datasets deposited in the SRA (Sequence Read Archive) database of the NCBI (National Center for Biotechnology Information). For more information about these two data sets, see: a) https://doi.org/10.1002/tpg2.20182 b) https://doi.org/10.1007/s43657-022-00061-2 We applied the bioinformatics platform OmicsBox v1.3 (BioBam, 2019) to perform the transcriptomics analysis. The two TRs and the respective sets of fastq files used for their production were subjected to differential expression analysis using edgeR v3.28.0 software (Robinson et al., 2010) in the context of OmicsBox v1.3 (BioBam, 2019). |
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https://doi.org/10.48432/39ZANHSouza Junior, Manoel TeixeiraLeão, André PereiraSousa, Carlos Antônio Ferreira deSalt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia).Genes responsivos ao estresse salino dos genomas de Portulaca oleracea (Purslane) e Gliricidia sepium (Gliricidia).RedapeBiotechnology-Based Assets – Saline stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia), ready to be cloned into a binary vector and later validated in a model plant or target plant to verify the ability to confer salinity tolerance by overexpression. The transcriptomics databases (RNASeq) of Portulaca oleracea (Beldroega) and Gliricidia sepium (Gliricidia), delivered to the Embrapa Agroenergia Pre-Technology Showcase in 2019 and 2020, underwent a selection process to identify saline stress-responsive genes. The databases were within the scope of the SI/CI "Establish a database of phenomics, genomics, transcriptomics and metabolomics of Palm Oil, focusing on the generation of information related to the response of this culture to abiotic and biotic stresses." The selection process led to 298 genes in purslane and 85 in gliricidia. This set of 383 saline stress-responsive genes constitutes a collection of Biotechnology-Based Assets to be validated for their ability to confer tolerance to this abiotic stress. Initially, we developed new versions of the Reference Transcripts (TR) of these two species using their respective datasets deposited in the SRA (Sequence Read Archive) database of the NCBI (National Center for Biotechnology Information). For more information about these two data sets, see: a) https://doi.org/10.1002/tpg2.20182 b) https://doi.org/10.1007/s43657-022-00061-2 We applied the bioinformatics platform OmicsBox v1.3 (BioBam, 2019) to perform the transcriptomics analysis. The two TRs and the respective sets of fastq files used for their production were subjected to differential expression analysis using edgeR v3.28.0 software (Robinson et al., 2010) in the context of OmicsBox v1.3 (BioBam, 2019).Ativos de Base Biotecnológica – Genes responsivos a estresse salino, oriundos dos genomas de Portulaca oleracea (Beldroega) e Gliricidia sepium (Gliricidia), prontos para serem clonados em vetor binário e posteriormente validados em planta modelo e/ou planta alvo para averiguar capacidade de conferir tolerância a salinidade mediante superexpressão. Foi realizado um processo de seleção de genes responsivos ao estresse salino nos Bancos de Dados de transcritômica (RNASeq) de Portulaca oleracea (Beldroega) e Gliricidia sepium (Gliricidia) entregues à Vitrine de Pré-Tecnologias da Embrapa Agroenergia em 2019 e 2020, respectivamente - resultados entregues no âmbito da SI/CI "Estabelecer banco de dados de fenômica, genômica, transcritômica e metabolômica de Palma de Óleo, com foco na geração de informações relacionadas à resposta desta cultura a estresses abióticos e bióticos". Este processo levou à seleção de 298 genes em beldroega e 85 em gliricidia. Este conjunto de 383 genes responsivos ao estresse salino constitui um acervo de Ativos de Base Biotecnológica a serem validados quanto à capacidade de conferir tolerância a este importante estresse abiótico. Inicialmente, foram geradas novas versões do Transcritomas de Referência (TR) destas duas espécies, utilizando seus respectivos conjuntos de dados depositados no banco de dados SRA (Sequence Read Archive) do NCBI (National Center for Biotechnology Information). Para maiores informações acerca destes dois conjuntos de dados, vide: a) https://doi.org/10.1002/tpg2.20182 b) https://doi.org/10.1007/s43657-022-00061-2 Toda a análise de transcritômica foi realizada na plataforma de bioinformática OmicsBox v1.3 (BioBam, 2019). Os dois TRs e os respectivos conjuntos de fastq files utilizados para a produção dos mesmos foram submetidos a análise de expressão diferencial utilizando o software edgeR v3.28.0 (Robinson et al., 2010) no contexto da OmicsBox v1.3 (BioBam, 2019).2022-11-03info:eu-repo/semantics/openAccesshttps://www.redape.dados.embrapa.br/licenses/embrapa-by-nc-4.0.xhtmlAgricultural Sciencesstress abióticosalinidadetolerância ao salPortulaca oleraceaGliricidia sepiumRNA sequencetranscriptomeinfo:eu-repo/semantics/datasetinfo:eu-repo/semantics/datasetinfo:eu-repo/semantics/publishedVersionDatasetreponame:Repositório de Dados de Pesquisa da EMBRAPA (Redape)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPARepositório de Dados de PesquisaPUBhttps://www.redape.dados.embrapa.br/oaiopendoar:2024-03-20T09:48:52.158483Repositório de Dados de Pesquisa da EMBRAPA (Redape) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)falsedoi:10.48432/39ZANH |
dc.title.none.fl_str_mv |
Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia). Genes responsivos ao estresse salino dos genomas de Portulaca oleracea (Purslane) e Gliricidia sepium (Gliricidia). |
title |
Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia). |
spellingShingle |
Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia). Souza Junior, Manoel Teixeira Agricultural Sciences stress abiótico salinidade tolerância ao sal Portulaca oleracea Gliricidia sepium RNA sequence transcriptome |
title_short |
Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia). |
title_full |
Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia). |
title_fullStr |
Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia). |
title_full_unstemmed |
Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia). |
title_sort |
Salt stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia). |
author |
Souza Junior, Manoel Teixeira |
author_facet |
Souza Junior, Manoel Teixeira Leão, André Pereira Sousa, Carlos Antônio Ferreira de |
author_role |
author |
author2 |
Leão, André Pereira Sousa, Carlos Antônio Ferreira de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Souza Junior, Manoel Teixeira Leão, André Pereira Sousa, Carlos Antônio Ferreira de |
dc.subject.none.fl_str_mv |
Agricultural Sciences stress abiótico salinidade tolerância ao sal Portulaca oleracea Gliricidia sepium RNA sequence transcriptome |
topic |
Agricultural Sciences stress abiótico salinidade tolerância ao sal Portulaca oleracea Gliricidia sepium RNA sequence transcriptome |
description |
Biotechnology-Based Assets – Saline stress-responsive genes from the genomes of Portulaca oleracea (Purslane) and Gliricidia sepium (Gliricidia), ready to be cloned into a binary vector and later validated in a model plant or target plant to verify the ability to confer salinity tolerance by overexpression. The transcriptomics databases (RNASeq) of Portulaca oleracea (Beldroega) and Gliricidia sepium (Gliricidia), delivered to the Embrapa Agroenergia Pre-Technology Showcase in 2019 and 2020, underwent a selection process to identify saline stress-responsive genes. The databases were within the scope of the SI/CI "Establish a database of phenomics, genomics, transcriptomics and metabolomics of Palm Oil, focusing on the generation of information related to the response of this culture to abiotic and biotic stresses." The selection process led to 298 genes in purslane and 85 in gliricidia. This set of 383 saline stress-responsive genes constitutes a collection of Biotechnology-Based Assets to be validated for their ability to confer tolerance to this abiotic stress. Initially, we developed new versions of the Reference Transcripts (TR) of these two species using their respective datasets deposited in the SRA (Sequence Read Archive) database of the NCBI (National Center for Biotechnology Information). For more information about these two data sets, see: a) https://doi.org/10.1002/tpg2.20182 b) https://doi.org/10.1007/s43657-022-00061-2 We applied the bioinformatics platform OmicsBox v1.3 (BioBam, 2019) to perform the transcriptomics analysis. The two TRs and the respective sets of fastq files used for their production were subjected to differential expression analysis using edgeR v3.28.0 software (Robinson et al., 2010) in the context of OmicsBox v1.3 (BioBam, 2019). |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-11-03 |
dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/dataset |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/dataset |
format |
dataset |
status_str |
publishedVersion |
dc.identifier.url.fl_str_mv |
https://doi.org/10.48432/39ZANH |
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https://doi.org/10.48432/39ZANH |
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info:eu-repo/semantics/openAccess https://www.redape.dados.embrapa.br/licenses/embrapa-by-nc-4.0.xhtml |
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openAccess |
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https://www.redape.dados.embrapa.br/licenses/embrapa-by-nc-4.0.xhtml |
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Dataset |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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Repositório de Dados de Pesquisa da EMBRAPA (Redape) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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